Výsledky vyhledávání - "Chihana Higashi"

The proper ratio of GrpE to DnaK is important for protein quality control by the DnaK–DnaJ–GrpE chaperone system and for cell division

Autor: Shinya Sugimoto, Chihana Higashi, Kozue Saruwatari, Kenji Sonomoto

Publikováno v: Microbiology. 154:1876-1885

A balance of the intracellular concentrations of molecular chaperones in response to environmental conditions is of considerable importance for cellular homeostasis. Here, the physiological consequences of overexpression of GrpE in wild-type Escheric

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1d92f4a99d978405f5581e8f469fa08
https://doi.org/10.1099/mic.0.2008/017376-0

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Bioavailability of vitamin C from mashed potatoes and potato chips after oral administration in healthy Japanese men

Autor: Mizuki Iwama, Yoshitaka Kondo, Chihana Higashi, Katsuyuki Ishihara, Setsuko Handa, Akihito Ishigami, Naoki Maruyama, Hidenori Koga, Hiroyuki Mugita

Publikováno v: The British journal of nutrition. 107(6)

Potato (Solanum tuberosum) tubers contain vitamin C (VC) and commercial potato chips have more VC content per wet weight by dehydration during frying. However, intestinal absorption of VC from orally ingested potatoes and its transfer to the blood re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e50bafa974a2cf30e1d46a46c5befff
https://pubmed.ncbi.nlm.nih.gov/21917196

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Improvement of Multiple-Stress Tolerance and Lactic Acid Production in Lactococcus lactis NZ9000 under Conditions of Thermal Stress by Heterologous Expression of Escherichia coli dnaK▿

Autor: Chihana Higashi, Abdullah-Al-Mahin, Shinya Sugimoto, Shunsuke Matsumoto, Kenji Sonomoto

The effects of nisin-induced dnaK expression in Lactococcus lactis were examined, and this expression was shown to improve stress tolerance and lactic acid fermentation efficiency. Using a nisin-inducible expression system, DnaK proteins from L. lact

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e52e499ac061115ad1af5e24fc406c09
https://europepmc.org/articles/PMC2897460/

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Construction of Escherichia coli dnaK-deletion mutant infected by lambdaDE3 for overexpression and purification of recombinant GrpE proteins

Autor: Shinya Sugimoto, Kenji Sonomoto, Chihana Higashi, Hiroyuki Yoshida

Publikováno v: Protein expression and purification. 60(1)

Escherichia coli is widely employed to produce recombinant proteins because this microorganism is simple to manipulate, inexpensive to culture, and of short duration to produce a recombinant protein. However, contamination of molecular chaperone DnaK

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35a38714a211ac2618138a763cd63114
https://pubmed.ncbi.nlm.nih.gov/18434193

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A gram-negative characteristic segment in Escherichia coli DnaK is essential for the ATP-dependent cooperative function with the co-chaperones DnaJ and GrpE

Autor: Chihana Higashi, Jiro Nakayama, Kenji Sonomoto, Kozue Saruwatari, Shinya Sugimoto

Publikováno v: FEBS letters. 581(16)

We describe importance of the characteristic segment in ATPase domain of DnaK chaperone which is present in all gram-negative bacteria but is absent in all gram-positive bacteria. In vitro studies, ATPase activity, luciferase-refolding activity, and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ab12a298f7acf97d066127feba5d655
https://pubmed.ncbi.nlm.nih.gov/17544398

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