Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Chiemi Takenaka"'
Autor:
Chiemi Takenaka, Hiroshi Miyajima, Yusuke Yoda, Hideo Imazato, Takako Yamamoto, Shinichi Gomi, Yasuhiro Ohshima, Kenichi Kagawa, Tetsuji Sasaki, Shin Kawamata
Publikováno v:
PLoS ONE, Vol 10, Iss 6, p e0129855 (2015)
Here, we introduce a new serum-free defined medium (SPM) that supports the cultivation of human pluripotent stem cells (hPSCs) on recombinant human vitronectin-N (rhVNT-N)-coated dishes after seeding with either cell clumps or single cells. With this
Externí odkaz:
https://doaj.org/article/c9a044f646d743a5adb6bc761c6fdbe5
Autor:
Keitaro Imaizumi, Naoki Nishishita, Marie Muramatsu, Takako Yamamoto, Chiemi Takenaka, Shin Kawamata, Kenichiro Kobayashi, Shin-Ichi Nishikawa, Teruo Akuta
Publikováno v:
PLoS ONE, Vol 9, Iss 2, p e88696 (2014)
Vitrification and slow-freezing methods have been used for the cryopreservation of human pluripotent stem cells (hPSCs). Vitrification requires considerable skill and post-thaw recovery is low. Furthermore, it is not suitable for cryopreservation of
Externí odkaz:
https://doaj.org/article/270e3345f4de4cd790ae12d154781d9f
Autor:
Naoki Nishishita, Masayuki Shikamura, Chiemi Takenaka, Nozomi Takada, Noemi Fusaki, Shin Kawamata
Publikováno v:
PLoS ONE, Vol 7, Iss 6, p e38389 (2012)
CD34+ cord blood cells can be reprogrammed effectively on dishes coated with a synthetic RGD motif polymer (PronectinF®) using a temperature sensitive Sendai virus vector (SeV TS7) carrying reprogramming factors OCT3/4, SOX2, KLF4 and c-MYC. Dish-sh
Externí odkaz:
https://doaj.org/article/fa2b918d537844da8d3b8962895a3054
Autor:
Naoki Nishishita, Masayuki Shikamura, Chiemi Takenaka, Nozomi Takada, Noemi Fusaki, Shin Kawamata
Publikováno v:
PLoS ONE, Vol 7, Iss 9 (2012)
Externí odkaz:
https://doaj.org/article/9e806819e8c0458494be1298e1732ba2
Autor:
Takako Yamamoto, Yoji Sato, Satoshi Yasuda, Masayuki Shikamura, Takashi Tamura, Chiemi Takenaka, Naoko Takasu, Masaki Nomura, Hiromi Dohi, Masayo Takahashi, Michiko Mandai, Yonehiro Kanemura, Masaya Nakamura, Hideyuki Okano, Shin Kawamata
Publikováno v:
Stem cells translational medicine. 11(5)
Cell therapy using induced pluripotent stem cell (iPSC) derivatives may result in abnormal tissue generation because the cells undergo numerous cycles of mitosis before clinical application, potentially increasing the accumulation of genetic abnormal
Autor:
Shin Kawamata, Oshima Yasuhiro, Tetsuji Sasaki, Takako Yamamoto, Yusuke Yoda, Hiroshi Miyajima, Chiemi Takenaka, Kenichi Kagawa
Publikováno v:
Scientific Reports
Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
Embryonic Stem Cells (ESC) possesses two distinct features; self-renewal and the potential to differentiate. Here we show the differentiation potential and growth rate of ESC correlates positively with the expression level of the gene encoding chromo
Autor:
Hajime Mori, Kenichiro Kobayashi, Naoki Nishishita, Shin Kawamata, Eiji Kotani, Chiemi Takenaka, Hiroshi Ijiri, Tohru Itoh, Kohei Goto
Publikováno v:
Biomaterials. 32:3555-3563
Human leukemia inhibitory factor (LIF) was immobilized into insect virus-derived microcrystals (polyhedra) to generate LIF polyhedra (LIF-PH) that can slowly release LIF into embryonic stem (ES) cell culture media and thus maintain ES cells in an und
Autor:
Keiko Ikeda, Shin Kawamata, Yoshito Ikada, Naoki Nishishita, Takashi Suyama, Hajime Mori, Chiemi Takenaka, Kentaro Matsushima, Lars Martin Jakt, Yoshiki Sawa
Publikováno v:
Tissue Engineering Part A. 16:3329-3341
Expression of the Wnt modulator secreted frizzled related protein 4 (Sfrp4) is upregulated after heart ischemic injury. We show that intramuscular administration of recombinant Sfrp4 to rat heart ischemic injury and recanalization models prevents fur
Publikováno v:
Experimental Cell Research. 314:1147-1154
Mesenchymal stem cells (MSCs) derived from human bone marrow have capability to differentiate into cells of mesenchymal lineage. The cells have already been applied in various clinical situations because of their expansion and differentiation capabil
Autor:
Hiroshi Miyajima, Shin Kawamata, Takako Yamamoto, Chiemi Takenaka, Hideo Imazato, Tetsuji Sasaki, Shinichi Gomi, Yusuke Yoda, Kenichi Kagawa, Yasuhiro Ohshima
Publikováno v:
PLoS ONE
PLoS ONE, Vol 10, Iss 6, p e0129855 (2015)
PLoS ONE, Vol 10, Iss 6, p e0129855 (2015)
Here, we introduce a new serum-free defined medium (SPM) that supports the cultivation of human pluripotent stem cells (hPSCs) on recombinant human vitronectin-N (rhVNT-N)-coated dishes after seeding with either cell clumps or single cells. With this