Výsledky vyhledávání - "Chie Komiyama"

FRET analysis of protein conformational change through position-specific incorporation of fluorescent amino acids

Autor: Ryoji Abe, Masahiko Sisido, Chie Komiyama, Takahiro Hohsaka, Issei Iijima, Daisuke Kajihara

Publikováno v: Nature Methods. 3:923-929

We designed and synthesized new, fluorescent, non-natural amino acids that emit fluorescence of wavelengths longer than 500 nm and are accepted by an Escherichia coli cell-free translation system. We synthesized p-aminophenylalanine derivatives linke

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24f8bbb74fb3f0d11b37826e8255fa11
https://doi.org/10.1038/nmeth945

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Position-specific incorporation of dansylated non-natural amino acids into streptavidin by using a four-base codon

Autor: Masahiko Sisido, Hiroshi Murakami, Chie Komiyama, Ryoji Abe, Kinue Matsui, Satomi Takaura, Norihito Muranaka, Takahiro Hohsaka

Publikováno v: FEBS letters. 560(1-3)

Novel non-natural amino acids carrying a dansyl fluorescent group were designed, synthesized, and incorporated into various positions of streptavidin by using a CGGG four-base codon in an Escherichia coli in vitro translation system. 2,6-Dansyl-amino

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1302f0dd4193ea3e2f1f218dc3e5236d
https://pubmed.ncbi.nlm.nih.gov/14988018

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