Zobrazeno 1 - 10
of 46
pro vyhledávání: '"Chidambaram Ramachandran"'
Autor:
Mei-Chu Lo, Bobbie Thomasson, Jason W. O'Neill, Chidambaram Ramachandran, Cesar Meleza, Klaus Michelsen
Publikováno v:
Analytical biochemistry. 511
Prostaglandin D2 synthase (PGDS) catalyzes the isomerization of prostaglandin H2 (PGH2) to prostaglandin D2 (PGD2). PGD2 produced by hematopoietic prostaglandin D2 synthase (H-PGDS) in mast cells and Th2 cells is proposed to be a mediator of allergic
Autor:
Joel Robichaud, Chidambaram Ramachandran, Jean-Pierre Falgueyret, Robert Zamboni, Pierre-Olivier Hétu, Denis Riendeau, Marc Ouellet
Publikováno v:
Archives of Biochemistry and Biophysics. 477:155-162
We have characterized the structures of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E2 synthase-1 (mPGES-1) in intact cells using bifunctional and photo-activatable crosslinking agents. A dimeric complex was detected for COX-2 by both cross
Autor:
Michel Therien, Cheuk K. Lau, Robert Zamboni, Tammy LeRiche, Chun Sing Li, Chidambaram Ramachandran, Deena Waddleton, Vouy Linh Truong, Kathryn Skorey
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 14:2319-2322
The synthesis of a novel radioactive peptidic photoaffinity probe for the PTP-1B enzyme as well as some SAR leading to the choice of this compound as a photoaffinity probe are presented.
Autor:
Sylvie Desmarais, Chidambaram Ramachandran, Yves Boie, Chi-Chung Chan, Kathryn Skorey, Claude Dufresne, Deena Waddleton, Wanda Cromlish, Ernest Asante-Appiah, Robert Gordon, Lijing Xu, Patrick Roy, Farnaz Forghani, Qingping Wang, Yves Leblanc, Zhaoyin Wang, Brian P. Kennedy
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 14:1039-1042
The SAR from our peptide libraries was exploited to design a series of potent deoxybenzoin PTP-1B inhibitors. The introduction of an ortho bromo substituent next to the difluoromethylphosphonate warhead gave up to 20-fold increase in potency compared
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1601:19-28
We have characterized some novel caged fluorescein diphosphates as photoactivatable, cell-permeable substrates for protein tyrosine phosphatases and explored their usefulness in identifying inhibitors of tyrosine phosphatases. 1-(2-Nitrophenyl)ethyl
Autor:
Claude Dufresne, Ernest Asante-Appiah, Yves Leblanc, Vira Patel, Richard Friesen, Chidambaram Ramachandran, Brian P. Kennedy, Giovanna Scapin, Joseph W. Becker, Patrick Roy, Qingping Wang, Sangita B. Patel
Publikováno v:
Biochemistry. 41:9043-9051
Inhibitors of PTP-1B could be therapeutically beneficial in the treatment of type 2 diabetes. Owing to the large number of phosphatases in the cell, inhibitors against PTP-1B must not only be potent but selective as well. N-Benzoyl-L-glutamyl-[4-phos
Autor:
Caroline Desponts, Paul Payette, Christopher I. Bayly, Kevin P. Bateman, Ernest Asante-Appiah, Kathryn Skorey, Chidambaram Ramachandran, Robert Zamboni, Giovanna Scapin, Brian P. Kennedy, R. W. Friesen, Kristen Ball
Publikováno v:
Journal of Biological Chemistry. 276:26036-26043
We have studied T-cell protein-tyrosine phosphatase (TCPTP) as a model phosphatase in an attempt to unravel amino acid residues that may influence the design of specific inhibitors. Residues 48--50, termed the YRD motif, a region that is found in pro
Publikováno v:
Analytical Biochemistry. 285:58-63
A simple, high-throughput fluorescent assay was developed to measure the inhibition of membrane-bound CD45 from Jurkat cells. This assay is based on the fact that approximately 64% of PTP activity from Jurkat cell membrane is contributed by CD45. Thi
Autor:
Sylvie Desmarais, Chidambaram Ramachandran, Qingping Wang, Robert Zamboni, John Scheigetz, Zheng Huang, Arvind Gorvindarajan, Hoa D. Ly
Publikováno v:
SLAS Discovery. 4:327-334
A highly sensitive and continuous protein tyrosine phosphatase (PTPase) assay using 3,6-fluorescein diphosphate (FDP) is described. Leukocyte phosphatase CD45 (leukocyte common antigen), protein tyrosine phosphatase-1B, and leukocyte common antigen-r
Autor:
Nancy Detich, Michael J. Gresser, Cary Cuncic, Sylvie Desmarais, Chidambaram Ramachandran, Alan S. Tracey
Publikováno v:
Biochemical Pharmacology. 58:1859-1867
We have shown previously that bis(N,N-dimethylhydroxamido)hydroxooxovanadate (DMHV) is an excellent reversible inhibitor of protein tyrosine phosphatase (PTP) in vitro. DMHV does not carry a charge under physiological pH conditions and is anticipated