Single-cell immunoblotting resolves estrogen receptor-α isoforms in breast cancer.

Autor: John J Kim, Wenchuan Liang, Chi-Chih Kang, Mark D Pegram, Amy E Herr

Publikováno v: PLoS ONE, Vol 16, Iss 7, p e0254783 (2021)

An array of isoforms of the nuclear estrogen receptor alpha (ER-α) protein contribute to heterogeneous response in breast cancer (BCa); yet, a single-cell analysis tool that distinguishes the full-length ER-α66 protein from the activation function-

Externí odkaz: https://doaj.org/article/9775623135fe4148b38d349814c57b13

Single cell–resolution western blotting

Autor: Kevin A. Yamauchi, Todd A. Duncombe, Julea Vlassakis, Chi-Chih Kang, Elly Sinkala, Amy E. Herr

Publikováno v: Nature Protocols. 11:1508-1530

This protocol describes how to perform western blotting on individual cells to measure cell-to-cell variation in protein expression levels and protein state. like conventional western blotting, single-cell western blotting (scWB) is particularly usef

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e682980e8357504727f40d505710580
https://doi.org/10.1038/nprot.2016.089

Linking Invasive Motility to Protein Expression in Single Tumor Cells

Autor: Jung-Ming G. Lin, Yun Zhou, Haiyan Huang, Sanjay Kumar, Amy E. Herr, Chi-Chih Kang

Publikováno v: Lab on a chip, vol 18, iss 2

The invasion of malignant cells into tissue is a critical step in the progression of cancer. While it is increasingly appreciated that cells within a tumor differ in their invasive potential, it remains nearly unknown how these differences relate to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c149c266c9cf3fb5ad8fb95fc6444ef
https://europepmc.org/articles/PMC5771853/

Single-Cell Western Blotting after Whole-Cell Imaging to Assess Cancer Chemotherapeutic Response

Autor: Chi-Chih Kang, Jung-Ming G. Lin, Zhuchen Xu, Amy E. Herr, Sanjay Kumar

Publikováno v: Analytical Chemistry
Kang, CC; Lin, JMG; Xu, Z; Kumar, S; & Herr, AE. (2014). Single-cell western blotting after whole-cell imaging to assess cancer chemotherapeutic response. Analytical Chemistry, 86(20), 10429-10436. doi: 10.1021/ac502932t. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/3xd4q33q
Analytical chemistry, vol 86, iss 20

© 2014 American Chemical Society. Intratumor heterogeneity remains a major obstacle to effective cancer therapy and personalized medicine. Current understanding points to differential therapeutic response among subpopulations of tumor cells as a key

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::295ed6a1a5704bfeae9f45d81a4baa06
https://doi.org/10.1021/ac502932t

BMVC test, an improved fluorescence assay for detection of malignant pleural effusions

Autor: Hao-Chien Wang, Huey-Dong Wu, Jin-Yuan Shih, I-Shiow Jan, Yu-Lin Tsai, Ta-Chau Chang, Pei-Jen Lou, Mei-Ying Lin, Chi-Chih Kang, Chiung-Lin Wang, I-Ting Lin, Tzu-Hsiu Tsai, Wei-Chun Huang

Publikováno v: Cancer Medicine

The diagnosis of malignant pleural effusions is an important issue in the management of malignancy patients. Generally, cytologic examination is a routine diagnostic technique. However, morphological interpretation of cytology is sometimes inconclusi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6e396bcef5d62b1f4a405eb9776a5bb
http://europepmc.org/articles/PMC3930401