Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Cheuk W Kan"'
Hypoxia due to cardiac arrest induces a time-dependent increase in serum amyloid β levels in humans.
Autor:
Henrik Zetterberg, Erik Mörtberg, Linan Song, Lei Chang, Gail K Provuncher, Purvish P Patel, Evan Ferrell, David R Fournier, Cheuk W Kan, Todd G Campbell, Ray Meyer, Andrew J Rivnak, Brian A Pink, Kaitlin A Minnehan, Tomasz Piech, David M Rissin, David C Duffy, Sten Rubertsson, David H Wilson, Kaj Blennow
Publikováno v:
PLoS ONE, Vol 6, Iss 12, p e28263 (2011)
Amyloid β (Aβ) peptides are proteolytic products from amyloid precursor protein (APP) and are thought to play a role in Alzheimer disease (AD) pathogenesis. While much is known about molecular mechanisms underlying cerebral Aβ accumulation in fami
Externí odkaz:
https://doaj.org/article/bd279e68e5df447f8caafa35e86ed2e2
Autor:
Jianli Zhang, Alexander D. Wiener, Raymond E. Meyer, Cheuk W. Kan, David M. Rissin, Bharathi Kolluru, Christopher George, Carmen I. Tobos, Dandan Shan, David C. Duffy
Publikováno v:
Analytical Chemistry.
Autor:
Tianhong Chen, Adiba Ubaidu, Scott Douglas, Samantha Carranza, Alexis Wong, Cheuk W. Kan, David C. Duffy
Publikováno v:
Journal of immunological methods. 508
We have developed an ultrasensitive multiplexed immunoassay using 384-well microtiter plates capable of detecting proteins at subfemtomolar concentrations that requires as little as 2.5 μL of sample. Arrays of up to 4 capture antibodies were pattern
Autor:
Danielle M. Svancara, Carmen I. Tobos, Roger Millington, Ray E. Meyer, Nicholas Collier, Alexander D. Wiener, David M. Rissin, Anna Comperchio, Christopher Warwick, Cheuk W. Kan, David C. Duffy
Publikováno v:
Lab on a Chip. 20:2122-2135
We report the development of digital enzyme-linked immunosorbent assays (ELISAs) based on single molecule arrays (Simoa) with improved sensitivities over conventional digital ELISA, enabling detection of proteins at sub-attomolar concentrations. The
Autor:
Cheuk W. Kan, David C. Duffy, David M. Rissin, Todd G. Campbell, Derek R. DuPont, Andrew J. Rivnak, Carlos Cabrera, David Fournier, Sean Sullivan, Melissa Gardel, Brian A. Pink, Matthew W. Fishburn, Tomasz Piech, Linan Song
Publikováno v:
Journal of Immunological Methods. 424:20-27
We report a system and assay for performing fully-automated measurement of 6 proteins simultaneously with single molecule sensitivity. The system combines handling of samples, reagents, and consumables, with a module for imaging single molecule array
Autor:
David H. Wilson, Purvish P. Patel, Linan Song, Andrew J. Rivnak, Brian A. Pink, Mary A. Till, Lei Chang, Cheuk W. Kan, David C. Duffy, Gail K. Provuncher, David Hanlon, Kaitlin A. Minnehan, David Fournier, William A. Faubion, Evan P. Ferrell, Todd G. Campbell
Publikováno v:
Journal of Immunological Methods. 372:177-186
The quantitative measurement of inflammatory cytokines in blood has been limited by insufficient sensitivity of conventional immunoassays. This limitation has prevented the widespread clinical monitoring of cytokine concentrations in chronic inflamma
Autor:
Cheuk W. Kan, Brian A. Pink, David H. Wilson, Purvish P. Patel, David C. Duffy, David M. Rissin, Linan Song, David Fournier, Evan P. Ferrell, Andrew J. Rivnak, Tomasz Piech, Gail K. Provuncher, Stuart C. Howes, Lei Chang, Kaitlin A. Minnehan, Todd G. Campbell
Publikováno v:
Analytical Chemistry. 83:2279-2285
We report a method for combining the detection of single molecules (digital) and an ensemble of molecules (analog) that is capable of detecting enzyme label from 10(-19) M to 10(-13) M, for use in high sensitivity enzyme-linked immunosorbent assays (
Autor:
Jeffrey Randall, Cheuk W. Kan, Andrew J. Rivnak, Tomasz Piech, Purvish P. Patel, David C. Duffy, David R. Walt, David Fournier, Gail K. Provuncher, Stuart C. Howes, Lei Chang, David M. Rissin, Linan Song, Todd G. Campbell, Evan P. Ferrell
Publikováno v:
Nature biotechnology
The ability to detect single protein molecules in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodie
Autor:
Erica Frew, Evan P. Ferrell, Tomasz Piech, Marcus Reinhardt, David M. Rissin, Volker von Einem, Heiko Sayer, Matthew W. Fishburn, Todd G. Campbell, Yao Chen, Cheuk W. Kan, David H. Wilson, David C. Duffy, Purvish P. Patel, Carlos Cabrera, Raymond E. Meyer, David Fournier, Claus Vielsack, Lei Chang, William McGuigan
Publikováno v:
Journal of laboratory automation. 21(4)
Disease detection at the molecular level is driving the emerging revolution of early diagnosis and treatment. A challenge facing the field is that protein biomarkers for early diagnosis can be present in very low abundance. The lower limit of detecti
Autor:
Matthew W. Fishburn, Les Walling, Tomasz Piech, Cheuk W. Kan, David C. Duffy, David Fournier, William McGuigan, Bill Gigante, David M. Rissin, Gary W. Watson, Raymond E. Meyer
Publikováno v:
SPIE Proceedings.
Quanterix and Stratec Biomedical have developed an instrument that enables the automated measurement of multiple proteins at concentration ~1000 times lower than existing immunoassays. The instrument is based on Quanterix’s proprietary Single Molec