Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Chen-Tran Hsu"'
Autor:
Jin-Jun Yue, Jin-Ling Yuan, Fu-Hui Wu, Yu-Hsuan Yuan, Qiao-Wei Cheng, Chen-Tran Hsu, Choun-Sea Lin
Publikováno v:
Frontiers in Genome Editing, Vol 3 (2021)
In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system, protoplasts are not only useful for rapidly validating the mutagenesis efficiency of various RNA-guided endonucleases, promoters, sgRNA
Externí odkaz:
https://doaj.org/article/4ea4bed635704f898f5a73bfb60234a6
Publikováno v:
Frontiers in Genome Editing, Vol 2 (2021)
Biotic diseases cause substantial agricultural losses annually, spurring research into plant pathogens and strategies to mitigate them. Nicotiana benthamiana is a commonly used model plant for studying plant–pathogen interactions because it is host
Externí odkaz:
https://doaj.org/article/b5d09718a2164e29884d281645d209a3
Autor:
Choun-Sea Lin, Chen-Tran Hsu, Yu-Hsuan Yuan, Po-Xing Zheng, Fu-Hui Wu, Qiao-Wei Cheng, Yu-Lin Wu, Ting-Li Wu, Steven Lin, Jin-Jun Yue, Ying-Huey Cheng, Shu-I Lin, Ming-Che Shih, Jen Sheen, Yao-Cheng Lin
Publikováno v:
Plant Physiology. 188:1917-1930
Wild tomatoes (Solanum peruvianum) are important genomic resources for tomato research and breeding. Development of a foreign DNA-free clustered regularly interspaced short palindromic repeat (CRISPR)-Cas delivery system has potential to mitigate pub
Publikováno v:
Methods in Molecular Biology ISBN: 9781071631300
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cb3869d16347be6db1c8d67d98f9ab6a
https://doi.org/10.1007/978-1-0716-3131-7_19
https://doi.org/10.1007/978-1-0716-3131-7_19
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2464
Protoplast transfection is widely used in plant research to rapidly evaluate RNA degradation, reporter assay, gene expression, subcellular localization, and protein-protein interactions. In order to successfully use protoplast transfection with the n
Publikováno v:
Methods in Molecular Biology ISBN: 9781071621639
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2a7f518a79b62d688261432cac8eb054
https://doi.org/10.1007/978-1-0716-2164-6_4
https://doi.org/10.1007/978-1-0716-2164-6_4
Autor:
Choun-Sea Lin, Ming-Che Shih, Yao-Cheng Lin, Jen Sheen, Yu-Hsuan Yuan, Qiao-Wei Cheng, Chen-Tran Hsu, Fu-Hui Wu, Steven Lin
Publikováno v:
CRISPR J
Versatile genome editing can be facilitated by the insertion of DNA sequences into specific locations. Current protocols involving clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins rely on low eff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::926867e8145c5c74030c4986ede3eacb
https://europepmc.org/articles/PMC9639242/
https://europepmc.org/articles/PMC9639242/
Autor:
Yu-Jung Cheng, Choun-Sea Lin, Wei-Fon Hung, Qiao-Wei Cheng, Stanton B. Gelvin, Yu-Hsua Yuan, Fu-Hui Wu, Lan-Ying Lee, Chen-Tran Hsu
Publikováno v:
Plant Molecular Biology. 101:355-371
Protoplasts can be used for genome editing using several different CRISPR systems, either separately or simultaneously, and that the resulting mutations can be recovered in regenerated non-chimaeric plants. Protoplast transfection and regeneration sy
Publikováno v:
Frontiers in Genome Editing
Frontiers in Genome Editing, Vol 2 (2021)
Frontiers in Genome Editing, Vol 2 (2021)
Biotic diseases cause substantial agricultural losses annually, spurring research into plant pathogens and strategies to mitigate them. Nicotiana benthamiana is a commonly used model plant for studying plant–pathogen interactions because it is host
Autor:
Qiao-Wei Cheng, Yesheng Zhang, Choun-Sea Lin, Ling-Hung Yang, Chen-Tran Hsu, Han C.W. Hsiao, Li-Jen Liao, Jin-Yuan Fu, Wan-Jung Chang, Ming-Che Shih, Fu-Hui Wu, Stanton B. Gelvin, Lan-Ying Lee, Chen-Ting Yu, Ru Zhang, Wen Wang
Publikováno v:
Plant Biotechnology Journal
Summary Plant protoplasts are useful for assessing the efficiency of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) mutagenesis. We improved the process of protoplast isolation and transfection