Zobrazeno 1 - 10
of 7 091
pro vyhledávání: '"Chemically-defined"'
Autor:
Mohammadjafar Hashemi, Ferdous B. Finklea, Hanna Hammons, Yuan Tian, Nathan Young, Emma Kim, Caroline Halloin, Wiebke Triebert, Robert Zweigerdt, Amit Kumar Mitra, Elizabeth A. Lipke
Publikováno v:
Bioactive Materials, Vol 43, Iss , Pp 423-440 (2025)
A reliable suspension-based platform for scaling engineered cardiac tissue (ECT) production from human induced pluripotent stem cells (hiPSCs) is crucial for regenerative therapies. Here, we compared the production and functionality of ECTs formed us
Externí odkaz:
https://doaj.org/article/52fce42f65614dd89c7a6701b6a9ef7b
Autor:
Kazuto Kimura, Masaya Tsukamoto, Hiroko Sugisaki, Miyuu Tanaka, Mitsuru Kuwamura, Yuki Matsumoto, Genki Ishihara, Kei Watanabe, Mika Okada, Mahito Nakanishi, Kikuya Sugiura, Shingo Hatoya
Publikováno v:
Regenerative Therapy, Vol 26, Iss , Pp 708-716 (2024)
Companion animals, such as felines and canines, could provide an excellent platform for translational research from veterinary to human medicine. However, the use of feline induced pluripotent stems (fiPSCs) of quality in basic or clinical research h
Externí odkaz:
https://doaj.org/article/1d90ec70e8ce495bb603d67d954053e1
Autor:
Giulia Maria Cattaneo, Ilaria Varotto-Boccazzi, Riccardo Molteni, Federico Ronchetti, Paolo Gabrieli, Jairo Alfonso Mendoza-Roldan, Domenico Otranto, Emanuele Montomoli, Claudio Bandi, Sara Epis
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024)
Abstract The development of media for cell culture is a major issue in the biopharmaceutical industry, for the production of therapeutics, immune-modulating molecules and protein antigens. Chemically defined media offer several advantages, as they ar
Externí odkaz:
https://doaj.org/article/c2f19a23ce9d43bf976f7b5c253e91e4
Autor:
Pablo G. Cataldo, María Paulina Urquiza Martínez, Julio Villena, Haruki Kitazawa, Lucila Saavedra, Elvira M. Hebert
Publikováno v:
Frontiers in Microbiology, Vol 15 (2024)
IntroductionLevilactobacillus brevis CRL 2013, a plant-derived lactic acid bacterium (LAB) with immunomodulatory properties, has emerged as an efficient producer of γ-aminobutyric acid (GABA). Notably, not all LAB possess the ability to produce GABA
Externí odkaz:
https://doaj.org/article/73862bc6bd28494eb4d7fb5214a90969
Autor:
Hongsheng Men
Publikováno v:
Biology, Vol 13, Iss 10, p 789 (2024)
Assisted reproductive technology has revolutionized our ability to genetically manipulate, maintain and rederive laboratory animals of biomedical importance; manipulate animal reproduction or genetics to boost production of farm animals; and improve
Externí odkaz:
https://doaj.org/article/1978d7e96d174113b9cbdc812432432f
Publikováno v:
Engineering in Life Sciences, Vol 24, Iss 4, Pp n/a-n/a (2024)
Abstract Innovations in viral vaccine manufacturing are crucial for pandemic preparedness and to meet ever‐rising global demands. For influenza, however, production still mainly relies on technologies established decades ago. Although modern produc
Externí odkaz:
https://doaj.org/article/3b6443a7217a4d3ab3fc613a0d6b24db
Autor:
Jay S. Sangha, Paul Barrett, Thomas P. Curtis, Aline Métris, Nicholas S. Jakubovics, Irina D. Ofiteru
Publikováno v:
Microbiology Spectrum, Vol 12, Iss 4 (2024)
ABSTRACTThe oral microbiome plays an important role in protecting oral health. Here, we established a controlled mixed-species in vitro biofilm model and used it to assess the impact of glucose and lactate on the ability of Streptococcus mutans, an a
Externí odkaz:
https://doaj.org/article/32f4c796451f480f87e68d176100cd23
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 30, Iss , Pp 221-234 (2023)
Lentiviral vectors (LVVs) play a critical role in gene delivery for ex vivo gene-modified cell therapies. However, the lack of scalable LVV production methods and the high cost associated with them may limit their use. In this work, we demonstrate th
Externí odkaz:
https://doaj.org/article/48ae0660cdeb4000b997e4d9f1973ccf
Autor:
Jennifer Goldmanns, Georg Andreas Röhling, Marie Kristine Lipa, Theresa Scholand, Alexander Deitert, Tobias May, Evangeline Priya Haas, Matthias Boy, Andrea Herold, Jochen Büchs
Publikováno v:
BMC Biotechnology, Vol 23, Iss 1, Pp 1-23 (2023)
Abstract Background One critical parameter in microbial cultivations is the composition of the cultivation medium. Nowadays, the application of chemically defined media increases, due to a more defined and reproducible fermentation performance than i
Externí odkaz:
https://doaj.org/article/3b613fbdea584f7e826514e691495b96
Autor:
Chalothon Amporn, Sarawut Duongmawong, Somchit Guntaprom, Wilasinee Srisanyong, Phalita Koonnadilokpot, Sirikanda Thanasuwan, Dechawut Bunyaluk, Juggrid Jugsumrit, Jakrit Yaeram
Publikováno v:
Science Technology and Engineering Journal (STEJ), Vol 8, Iss 2, Pp 67-75 (2022)
This study examined the effect of a chemically defined media system and co-culture system on the in vitro production of porcine embryos. Cumulus-oocyte complexes (COCs) from porcine ovaries were cultured for 22 h in TCM-199 with Earle’s salts, L-gl
Externí odkaz:
https://doaj.org/article/b010402f8c3842b0b73ff755760941d6