Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Chee Wee Lee"'
Autor:
Lay-Beng Goh, Chee-Wee Lee
Publikováno v:
Biochemical Journal. 327:31-36
MCF-7 cells display both nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative, but not the Na+-dependent, nucleoside transport. Transport of uridine by es is more sensitive to inhibition by purine nucleosides, wherea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c1935e80479374d46a817cf2db67e46
https://doi.org/10.1042/bj3270031
https://doi.org/10.1042/bj3270031
Autor:
Jun Cai, Chee-Wee Lee
Publikováno v:
Biochemical Journal. 320:991-995
Tamoxifen inhibits the binding of [3H]nitrobenzylthioinosine ([3H]NBMPR) to human MCF-7 breast cancer cells with an IC50 of 8 microM. Tamoxifen at 30 microM changed the apparent Kd for [3H]NBMPR binding from 0.63 +/- 0.12 to 4.75 +/- 0.58 nM, with li
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44b347bba7de0deb61135b3f34fcd4a3
https://doi.org/10.1042/bj3200991
https://doi.org/10.1042/bj3200991
Publikováno v:
Journal of Chromatography B: Biomedical Sciences and Applications. 677:152-155
The conventional method for measuring urinary leukotriene E 4 (LTE4) is by reversed-phase high-performance liquid chromatography (RP-HPLC), followed by radioimmunoassay (RIA) or enzyme immunoassay (EIA). We measured urinary LTE4 levels by two methods
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b6866451dfcefa766a37ad0dbeb8b43
https://doi.org/10.1016/0378-4347(95)00468-8
https://doi.org/10.1016/0378-4347(95)00468-8
Publikováno v:
Biochemical Journal. 294:693-697
N-Formyl-Met-Leu-Phe (FMLP), at concentrations as low as 5 nM, caused an increase in intracellular uridine pools in dimethyl sulphoxide (Me2SO)-differentiated HL-60 cells. Intracellular uridine pools were elevated rapidly and reached a maximum within
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f605b077e4a773d32b9842bf236d8225
https://doi.org/10.1042/bj2940693
https://doi.org/10.1042/bj2940693
Autor:
Chee-Wee Lee
The dose-response curves for the inhibition of equilibrative uridine transport by dilazep, dipyridamole and nitrobenzylthioinosine (NBMPR) in undifferentiated HL-60 cells were biphasic. Some 70% of the transport activity was inhibited with IC50 value
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8d6808c3af201fbccca4668dfe4378e
https://europepmc.org/articles/PMC1138177/
https://europepmc.org/articles/PMC1138177/
The effects of pertussis toxin on the Na(+)-dependent transport of uridine were studied in HL-60 leukaemia cells induced to differentiate along the granulocytic or monocytic pathways by dimethyl sulphoxide (DMSO) or phorbol 12-myristate 13-acetate (P
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ec5480f761e64d6a4a51add4dbdde51
https://europepmc.org/articles/PMC1130578/
https://europepmc.org/articles/PMC1130578/
The Na(+)-dependent transport and facilitated diffusion of uridine were measured after differentiation of HL-60 leukaemia cells along the monocytic pathway by phorbol 12-myristate 13-acetate (PMA). PMA (200 ng/ml) caused a marked increase in Na(+)-de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fd5c6f8e79fb653d7fa341acffe8ff9
https://europepmc.org/articles/PMC1149923/
https://europepmc.org/articles/PMC1149923/
Publikováno v:
Leukemia research. 15(11)
HL-60 leukemia cells, induced to differentiate, activate a Na+-dependent nucleoside transport system, concomitant with a reduction in the nitrobenzylthioinosine (NBMPR)-sensitive facilitated transport of nucleosides. The consequence of these changes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27d12fdbd9e5b9f91fe65dce68688685
https://pubmed.ncbi.nlm.nih.gov/1961008
https://pubmed.ncbi.nlm.nih.gov/1961008
Publikováno v:
The American journal of physiology. 258(5 Pt 2)
The uptake of uridine into rat renal brush-border membrane vesicles is mediated by Na(+)- and K(+)-dependent concentrative transport processes. At a 100 mM extravesicular cation concentration the apparent Km values were 9.7 +/- 4.2 and 28 +/- 5 micro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c992fbac1f7f1ec4f97d327229cdace0
https://pubmed.ncbi.nlm.nih.gov/2337150
https://pubmed.ncbi.nlm.nih.gov/2337150
Autor:
Simon M. Jarvis, Chee Wee Lee
Publikováno v:
Biochemical Journal. 249:557-564
The transport of [U-14C]uridine was investigated in rat cerebral-cortical synaptosomes using an inhibitor-stop filtration method. Under these conditions the rapid efflux of uridine from the synaptosomes is prevented and uridine is not significantly m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e5cc850b4a5bf0268366c6b2734f6cf
https://doi.org/10.1042/bj2490557
https://doi.org/10.1042/bj2490557