Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Che Lin Kim"'
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
Abstract Cell surface heparan sulfate proteoglycan (HSPG)-mediated endocytosis results in poor yields of recombinant human bone morphogenetic proteins (rhBMPs) from CHO cell cultures. Upon incubation of rhBMP-2 and rhBMP-7 with CHO cells at 37 °C, b
Externí odkaz:
https://doaj.org/article/a7f876b8bcde4ab1807b9a3c638cb120
Autor:
Tae Kwang Ha, Andreu Òdena, Karen Julie la Cour Karottki, Che Lin Kim, Hooman Hefzi, Gyun Min Lee, Helene Faustrup Kildegaard, Lars K. Nielsen, Lise Marie Grav, Nathan E. Lewis
The dominant method for generating Chinese hamster ovary (CHO) cell lines that produce high titers of biotherapeutic proteins utilizes selectable markers such as dihydrofolate reductase (Dhfr) or glutamine synthetase (Gs), alongside inhibitory compou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0f1bc0d7cf24d24464749471b5974c5
https://doi.org/10.22541/au.165606842.29880741/v1
https://doi.org/10.22541/au.165606842.29880741/v1
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
Kim, M G, Kim, C L, Kim, Y S, Jang, J W & Lee, G M 2021, ' Selective endocytosis of recombinant human BMPs through cell surface heparan sulfate proteoglycans in CHO cells : BMP-2 and BMP-7 ', Scientific Reports, vol. 11, no. 1, 3378 . https://doi.org/10.1038/s41598-021-82955-1
Scientific Reports
Kim, M G, Kim, C L, Kim, Y S, Jang, J W & Lee, G M 2021, ' Selective endocytosis of recombinant human BMPs through cell surface heparan sulfate proteoglycans in CHO cells : BMP-2 and BMP-7 ', Scientific Reports, vol. 11, no. 1, 3378 . https://doi.org/10.1038/s41598-021-82955-1
Scientific Reports
Cell surface heparan sulfate proteoglycan (HSPG)-mediated endocytosis results in poor yields of recombinant human bone morphogenetic proteins (rhBMPs) from CHO cell cultures. Upon incubation of rhBMP-2 and rhBMP-7 with CHO cells at 37 °C, both rhBMP
Autor:
Che Lin Kim, Gyun Min Lee
Publikováno v:
Metabolic Engineering. 52:57-67
A Chinese hamster ovary (CHO) cell line producing recombinant human bone morphogenetic protein-4 (rhBMP-4) (CHO-BMP-4), which expresses essential components of BMP signal transduction, underwent autocrine BMP-4 signaling. RNA seq analysis on CHO host
Publikováno v:
Biotechnology advances. 54
Chinese hamster ovary (CHO) cells are the most widely used mammalian host cells for the commercial production of therapeutic proteins. Fed-batch culture is widely used to produce therapeutic proteins, including monoclonal antibodies, because of its o
Publikováno v:
Biotechnology and Bioengineering. 115:2565-2575
Endocytic regulation serves a critical role in modulating the extracellular level of signaling molecules, such as bone morphogenetic proteins (BMPs). Unfortunately, endocytosis may result in poor yields of recombinant human BMP-4 (rhBMP-4) from Chine
Publikováno v:
Biotechnology and Bioprocess Engineering. 21:667-675
Lithium chloride (LiCl), which induces cell cycle arrest at G2/M phase, is known as a specific production rate (qp)-enhancing additive in recombinant Chinese hamster ovary (CHO) cell culture. To determine the potential of LiCl as a chemical additive
Publikováno v:
Process Biochemistry. 51:1078-1084
The effects of culture temperature and pH on cell growth and recombinant human bone morphogenetic protein-4 (rhBMP-4) production were investigated to maximize rhBMP-4 production from Chinese hamster ovary cells. Cells were cultivated in a bioreactor
Publikováno v:
Biotechnology and Bioengineering. 113:1953-1961
Chinese hamster ovary (CHO) cells activate and undergo apoptosis and autophagy for various environmental stresses. Unlike apoptosis, studies on increasing the production of therapeutic proteins in CHO cells by targeting the autophagy pathway are limi
Publikováno v:
Biotechnology and Bioengineering. 112:560-568
Bone morphogenetic protein-7 (BMP-7) is synthesized as a precursor that requires proteolytic cleavage of the propeptide by proprotein convertases (PCs) for its functional activity. A high-level expression of BMP-7 in CHO cells (CHO-BMP-7) resulted in