Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans.

Autor: Chauve, Laetitia¹ (AUTHOR), Hodge, Francesca¹ (AUTHOR), Murdoch, Sharlene¹ (AUTHOR), Masoudzadeh, Fatemah¹ (AUTHOR), Mann, Harry-Jack² (AUTHOR), Lopez-Clavijo, Andrea² (AUTHOR), Okkenhaug, Hanneke² (AUTHOR), West, Greg² (AUTHOR), Sousa, Bebiana C.² (AUTHOR), Segonds-Pichon, Anne² (AUTHOR), Li, Cheryl¹ (AUTHOR), Wingett, Steven² (AUTHOR), Kienberger, Hermine³ (AUTHOR), Kleigrewe, Karin³ (AUTHOR), De Bono, Mario⁴ (AUTHOR), Wakelam, Michael⁵ (AUTHOR), Casanueva, Olivia¹ (AUTHOR) moc771@gmail.com

Publikováno v: PLoS Biology. 11/1/2021, Vol. 19 Issue 11, p1-33. 33p. 1 Color Photograph, 1 Diagram, 1 Chart, 4 Graphs.

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

Autor: Chauve, Laetitia, Le Pen, Jérémie, Hodge, Francesca, Todtenhaupt, Pia, Biggins, Laura, Miska, Eric A, Andrews, Simon, Casanueva, Olivia

This paper presents a high-throughput reverse transcription quantitative PCR (RT-qPCR) assay for Caenorhabditis elegans that is fast, robust, and highly sensitive. This protocol obtains precise measurements of gene expression from single worms or fro

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E2F coregulates an essential HSF developmental program that is distinct from the heat-shock response.

Autor: Jian Li¹, Chauve, Laetitia¹, Phelps, Grace¹, Brielmann, Renée M.¹, Morimoto, Richard I.¹

Publikováno v: Genes & Development. 9/15/2016, Vol. 30 Issue 18, p2062-2075. 14p.

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology.

Autor: Chauve L; Babraham Institute., Le Pen J; Gurdon Institute, University of Cambridge; Department of Genetics, University of Cambridge; Laboratory of Virology and Infectious Disease, The Rockefeller University., Hodge F; Babraham Institute., Todtenhaupt P; Babraham Institute., Biggins L; Babraham Institute., Miska EA; Gurdon Institute, University of Cambridge; Department of Genetics, University of Cambridge; Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute., Andrews S; Babraham Institute., Casanueva O; Babraham Institute; olivia.casanueva@babraham.ac.uk.

Publikováno v: Journal of visualized experiments : JoVE [J Vis Exp] 2020 May 28 (159). Date of Electronic Publication: 2020 May 28.