O -glycosylation of intrinsically disordered regions regulates homeostasis of membrane proteins in streptococci.

Autor: Rahman MM; Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, Kentucky, USA., Zamakhaeva S; Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, Kentucky, USA., Rush JS; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, USA., Chaton CT; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, USA., Kenner CW; Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, Kentucky, USA., Hla YM; Department of Biology, Indiana University Bloomington, Bloomington, Indiana, USA., Tsui HT; Department of Biology, Indiana University Bloomington, Bloomington, Indiana, USA., Uversky VN; Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, Florida, USA., Winkler ME; Department of Biology, Indiana University Bloomington, Bloomington, Indiana, USA., Korotkov KV; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, USA., Korotkova N; Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, Kentucky, USA.; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 Jul 09. Date of Electronic Publication: 2024 Jul 09.

Modification of cell wall polysaccharide guides cell division in Streptococcus mutans.

Autor: Zamakhaeva S; Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY, USA., Chaton CT; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA., Rush JS; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA., Ajay Castro S; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., Kenner CW; Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY, USA., Yarawsky AE; Divisions of Immunobiology and Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA., Herr AB; Divisions of Immunobiology and Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA., van Sorge NM; Department of Medical Microbiology and Infection Prevention, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The Netherlands.; Netherlands Reference Laboratory for Bacterial Meningitis, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The Netherlands., Dorfmueller HC; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., Frolenkov GI; Department of Physiology, University of Kentucky, Lexington, KY, USA., Korotkov KV; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA., Korotkova N; Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY, USA. nkorotkova@uky.edu.; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA. nkorotkova@uky.edu.

Publikováno v: Nature chemical biology [Nat Chem Biol] 2021 Aug; Vol. 17 (8), pp. 878-887. Date of Electronic Publication: 2021 May 27.

Structural analysis of mycobacterial homoserine transacetylases central to methionine biosynthesis reveals druggable active site.

Autor: Chaton CT; Department of Molecular & Cellular Biochemistry and the Center for Structural Biology, University of Kentucky, Lexington, KY, 40536, USA., Rodriguez ES; Department of Molecular & Cellular Biochemistry and the Center for Structural Biology, University of Kentucky, Lexington, KY, 40536, USA.; Department of Chemistry & Biochemistry, Ohio State University, Columbus, OH, 43210, USA., Reed RW; Department of Molecular & Cellular Biochemistry and the Center for Structural Biology, University of Kentucky, Lexington, KY, 40536, USA.; Division of Regulatory Services, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY, 40546, USA., Li J; Department of Molecular & Cellular Biochemistry and the Center for Structural Biology, University of Kentucky, Lexington, KY, 40536, USA.; Gannan Medical University, Ganzhou, Jiangxi, 341000, China., Kenner CW; Department of Molecular & Cellular Biochemistry and the Center for Structural Biology, University of Kentucky, Lexington, KY, 40536, USA.; Georgetown College, Georgetown, KY, 40324, USA., Korotkov KV; Department of Molecular & Cellular Biochemistry and the Center for Structural Biology, University of Kentucky, Lexington, KY, 40536, USA. kkorotkov@uky.edu.

Publikováno v: Scientific reports [Sci Rep] 2019 Dec 30; Vol. 9 (1), pp. 20267. Date of Electronic Publication: 2019 Dec 30.

Structural Variability of EspG Chaperones from Mycobacterial ESX-1, ESX-3, and ESX-5 Type VII Secretion Systems.

Autor: Tuukkanen AT; European Molecular Biology Laboratory, Hamburg Unit, Hamburg 22607, Germany., Freire D; European Molecular Biology Laboratory, Hamburg Unit, Hamburg 22607, Germany., Chan S; UCLA-DOE Institute, University of California Los Angeles, Los Angeles, CA 90095, USA., Arbing MA; UCLA-DOE Institute, University of California Los Angeles, Los Angeles, CA 90095, USA., Reed RW; Department of Molecular & Cellular Biochemistry, and Center for Structural Biology, University of Kentucky, Lexington, KY 40536, USA., Evans TJ; Department of Molecular & Cellular Biochemistry, and Center for Structural Biology, University of Kentucky, Lexington, KY 40536, USA., Zenkeviciutė G; European Molecular Biology Laboratory, Hamburg Unit, Hamburg 22607, Germany., Kim J; UCLA-DOE Institute, University of California Los Angeles, Los Angeles, CA 90095, USA., Kahng S; UCLA-DOE Institute, University of California Los Angeles, Los Angeles, CA 90095, USA., Sawaya MR; UCLA-DOE Institute, University of California Los Angeles, Los Angeles, CA 90095, USA., Chaton CT; Department of Molecular & Cellular Biochemistry, and Center for Structural Biology, University of Kentucky, Lexington, KY 40536, USA., Wilmanns M; European Molecular Biology Laboratory, Hamburg Unit, Hamburg 22607, Germany., Eisenberg D; UCLA-DOE Institute, University of California Los Angeles, Los Angeles, CA 90095, USA., Parret AHA; European Molecular Biology Laboratory, Hamburg Unit, Hamburg 22607, Germany. Electronic address: parret@embl-hamburg.de., Korotkov KV; Department of Molecular & Cellular Biochemistry, and Center for Structural Biology, University of Kentucky, Lexington, KY 40536, USA. Electronic address: kkorotkov@uky.edu.

Publikováno v: Journal of molecular biology [J Mol Biol] 2019 Jan 18; Vol. 431 (2), pp. 289-307. Date of Electronic Publication: 2018 Nov 10.

A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.

Autor: Zhao H; Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, 20892, United States of America., Ghirlando R; Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, 20892, United States of America., Alfonso C; Analytical Ultracentrifugacion and Light Scattering Facility, Centro de Investigaciones Biológicas, CSIC, Madrid, 28040, Spain., Arisaka F; Life Science Research Center, Nihon University, College of Bioresource Science, Fujisawa, 252-0880, Japan., Attali I; Department of Biochemistry and Molecular Biology, Tel Aviv University, Tel Aviv, 69978, Israel., Bain DL; Department of Pharmaceutical Sciences, University of Colorado Denver Anschutz Medical Campus, Aurora, Colorado, 80045, United States of America., Bakhtina MM; Department of Chemistry and Biochemistry, Center for Retrovirus Research, and Center for RNA Biology, The Ohio State University, Columbus, Ohio, 43210, United States of America., Becker DF; Redox Biology Center, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, United States of America., Bedwell GJ; Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, 35294, United States of America., Bekdemir A; Supramolecular Nanomaterials and Interfaces Laboratory, Institute of Materials, École Polytechnique Fédérale de Lausanne, Lausanne, CH-1015, Switzerland., Besong TM; National Centre for Macromolecular Hydrodynamics, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, United Kingdom., Birck C; Structural Biology Platform IGBMC, Illkirch, 67400, France., Brautigam CA; Department of Biophysics, The University of Texas Southwestern Medical Center, Dallas, Texas, 75390, United States of America., Brennerman W; Beckman Coulter, Inc., Life Science Division, Indianapolis, Indiana, 46268, United States of America., Byron O; School of Life Sciences, University of Glasgow, Glasgow, G37TT, United Kingdom., Bzowska A; Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Warsaw, 02-089, Poland., Chaires JB; JG Brown Cancer Center, University of Louisville, Louisville, Kentucky, 40202, United States of America., Chaton CT; Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio, 45267, United States of America., Cölfen H; Physical Chemistry, University of Konstanz, 78457, Konstanz, Germany., Connaghan KD; Department of Pharmaceutical Sciences, University of Colorado Denver Anschutz Medical Campus, Aurora, Colorado, 80045, United States of America., Crowley KA; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, 01605, United States of America., Curth U; Institute for Biophysical Chemistry, Hannover Medical School, 30625, Hannover, Germany., Daviter T; Institute of Structural and Molecular Biology Biophysics Centre, Birkbeck, University of London and University College London, London, WC1E 7HX, United Kingdom., Dean WL; JG Brown Cancer Center, University of Louisville, Louisville, Kentucky, 40202, United States of America., Díez AI; Department of Physical Chemistry, University of Murcia, Murcia, 30071, Spain., Ebel C; Univ. Grenoble Alpes, IBS, F-38044, Grenoble, France; CNRS, IBS, F-38044, Grenoble, France; CEA, IBS, F-38044, Grenoble, France., Eckert DM; Protein Interactions Core, Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, 84112, United States of America., Eisele LE; Wadsworth Center, New York State Department of Health, Albany, New York, 12208, United States of America., Eisenstein E; Institute for Bioscience and Biotechnology Research, Fischell Department of Bioengineering, University of Maryland, Rockville, Maryland, 20850, United States of America., England P; Institut Pasteur, Centre of Biophysics of Macromolecules and Their Interactions, Paris, 75724, France., Escalante C; Department of Physiology and Biophysics, School of Medicine, Virginia Commonwealth University, Richmond, Virginia, 23220, United States of America., Fagan JA; Materials Science and Engineering Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, 20899, United States of America., Fairman R; Department of Biology, Haverford College, Haverford, Pennsylvania, 19041, United States of America., Finn RM; Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077, Göttingen, Germany., Fischle W; Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077, Göttingen, Germany., de la Torre JG; Department of Physical Chemistry, University of Murcia, Murcia, 30071, Spain., Gor J; Department of Structural and Molecular Biology, Darwin Building, University College London, London, WC1E 6BT, United Kingdom., Gustafsson H; Diabetes Biophysics, Novo Nordisk A/S, Måløv, 2760, Denmark., Hall D; Research School of Chemistry, Section on Biological Chemistry, The Australian National University, Acton, ACT 0200, Australia., Harding SE; National Centre for Macromolecular Hydrodynamics, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, United Kingdom., Cifre JG; Department of Physical Chemistry, University of Murcia, Murcia, 30071, Spain., Herr AB; Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio, 45267, United States of America., Howell EE; Biochemistry, Cell and Molecular Biology Department, University of Tennessee, Knoxville, Tennessee, 37996-0840, United States of America., Isaac RS; Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, 94158, United States of America; Tetrad Graduate Program, University of California San Francisco, San Francisco, California, 94158, United States of America., Jao SC; Institute of Biological Chemistry, Academia Sinica, Taipei, 115, Taiwan; Biophysics Core Facility, Scientific Instrument Center, Academia Sinica, Taipei, 115, Taiwan., Jose D; Institute of Molecular Biology and Department of Chemistry and Biochemistry, University of Oregon, Eugene, Oregon, 97403, United States of America., Kim SJ; Department of Chemistry, Mokpo National University, Muan, 534-729, Korea., Kokona B; Department of Biology, Haverford College, Haverford, Pennsylvania, 19041, United States of America., Kornblatt JA; Enzyme Research Group, Concordia University, Montreal, Quebec, H4B 1R6, Canada., Kosek D; Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, Prague, 12843, Czech Republic., Krayukhina E; Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan., Krzizike D; Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado, 80523, United States of America., Kusznir EA; Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-LaRoche Ltd., Basel, 4070, Switzerland., Kwon H; Analytical Biopharmacy Core, University of Washington, Seattle, Washington, 98195, United States of America., Larson A; Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, 94158, United States of America; Tetrad Graduate Program, University of California San Francisco, San Francisco, California, 94158, United States of America., Laue TM; Department of Biochemistry, University of New Hampshire, Durham, New Hampshire, 03824, United States of America., Le Roy A; Univ. Grenoble Alpes, IBS, F-38044, Grenoble, France; CNRS, IBS, F-38044, Grenoble, France; CEA, IBS, F-38044, Grenoble, France., Leech AP; Technology Facility, Department of Biology, University of York, York, YO10 5DD, United Kingdom., Lilie H; Institute of Biochemistry and Biotechnology, Martin-Luther University Halle-Wittenberg, 06120, Halle, Germany., Luger K; Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado, 80523, United States of America., Luque-Ortega JR; Analytical Ultracentrifugacion and Light Scattering Facility, Centro de Investigaciones Biológicas, CSIC, Madrid, 28040, Spain., Ma J; Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, 20892, United States of America., May CA; Department of Biochemistry, University of New Hampshire, Durham, New Hampshire, 03824, United States of America., Maynard EL; Department of Biochemistry, Uniformed Services University of the Health Sciences, Bethesda, Maryland, 20814, United States of America., Modrak-Wojcik A; Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Warsaw, 02-089, Poland., Mok YF; Department of Biochemistry and Molecular Biology, Bio21 Instute of Molecular Science and Biotechnology, University of Melbourne, Parkville, 3010, Victoria, Australia., Mücke N; Biophysics of Macromolecules, German Cancer Research Center, Heidelberg, 69120, Germany., Nagel-Steger L; ICS-6, Structural Biochemistry, Research Center Juelich, 52428, Juelich, Germany., Narlikar GJ; Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, 94158, United States of America., Noda M; Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan., Nourse A; Molecular Interaction Analysis Shared Resource, St. Jude Children's Research Hospital, Memphis, Tennessee, 38105, United States of America., Obsil T; Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, Prague, 12843, Czech Republic., Park CK; Analytical Biophysics & Materials Characterization, Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona, 85721, United States of America., Park JK; Central Instrument Center, Mokpo National University, Muan, 534-729, Korea., Pawelek PD; Department of Chemistry and Biochemistry, Concordia University, Montreal, Quebec, H4B 1R6, Canada., Perdue EE; Beckman Coulter, Inc., Life Science Division, Indianapolis, Indiana, 46268, United States of America., Perkins SJ; Department of Structural and Molecular Biology, Darwin Building, University College London, London, WC1E 6BT, United Kingdom., Perugini MA; Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, 3086, Australia., Peterson CL; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, 01605, United States of America., Peverelli MG; Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, 3086, Australia., Piszczek G; Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, 20892, United States of America., Prag G; Department of Biochemistry and Molecular Biology, Tel Aviv University, Tel Aviv, 69978, Israel., Prevelige PE; Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, 35294, United States of America., Raynal BD; Institut Pasteur, Centre of Biophysics of Macromolecules and Their Interactions, Paris, 75724, France., Rezabkova L; Laboratory of Biomolecular Research, Department of Biology and Chemistry, Paul Scherrer Institute, 5232, Villigen PSI, Switzerland., Richter K; Department of Chemistry and Center for Integrated Protein Science, Technische Universität München, 85748, Garching, Germany., Ringel AE; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205, United States of America., Rosenberg R; Physical Chemistry, University of Konstanz, 78457, Konstanz, Germany., Rowe AJ; National Centre for Macromolecular Hydrodynamics, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, United Kingdom., Rufer AC; Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-LaRoche Ltd., Basel, 4070, Switzerland., Scott DJ; Research Complex at Harwell, Rutherford Appleton Laboratory, Oxfordshire, OX11 0FA, United Kingdom., Seravalli JG; Redox Biology Center, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, United States of America., Solovyova AS; Proteome and Protein Analysis, University of Newcastle, Newcastle upon Tyne, NE1 7RU, United Kingdom., Song R; Wadsworth Center, New York State Department of Health, Albany, New York, 12208, United States of America., Staunton D; Molecular Biophysics Suite, Department of Biochemistry, Oxford, Oxon, OX1 3QU, United Kingdom., Stoddard C; Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, 94158, United States of America; Tetrad Graduate Program, University of California San Francisco, San Francisco, California, 94158, United States of America., Stott K; Biochemistry Department, University of Cambridge, Cambridge, CB2 1GA, United Kingdom., Strauss HM; Diabetes Biophysics, Novo Nordisk A/S, Måløv, 2760, Denmark., Streicher WW; Protein Function and Interactions, Novo Nordisk Foundation Center for Protein Research, Copenhagen, 2200, Denmark., Sumida JP; Analytical Biopharmacy Core, University of Washington, Seattle, Washington, 98195, United States of America., Swygert SG; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, 01605, United States of America., Szczepanowski RH; Core Facility, International Institute of Molecular and Cell Biology, Warsaw, 02-109, Poland., Tessmer I; Rudolf Virchow Center for Experimental Biomedicine, University of Würzburg, 97080, Würzburg, Germany., Toth RT 4th; Macromolecule and Vaccine Stabilization Center, University of Kansas, Lawrence, Kansas, 66047, United States of America., Tripathy A; Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599, United States of America., Uchiyama S; Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan., Uebel SF; Biochemistry Core Facility, Max Planck Institute of Biochemistry, 82152, Martinsried, Germany., Unzai S; Drug Design Laboratory, Graduate School of Medical Life Science, Yokohama City University, Yokohama, 230-0045, Japan., Gruber AV; Department of Biochemistry and Molecular Biology, Tel Aviv University, Tel Aviv, 69978, Israel., von Hippel PH; Institute of Molecular Biology and Department of Chemistry and Biochemistry, University of Oregon, Eugene, Oregon, 97403, United States of America., Wandrey C; Laboratoire de Médecine Régénérative et de Pharmacobiologie, Ecole Polytechnique Fédérale de Lausanne, Lausanne, CH-1015, Switzerland., Wang SH; Biophysics Core Facility, Scientific Instrument Center, Academia Sinica, Taipei, 115, Taiwan., Weitzel SE; Institute of Molecular Biology and Department of Chemistry and Biochemistry, University of Oregon, Eugene, Oregon, 97403, United States of America., Wielgus-Kutrowska B; Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Warsaw, 02-089, Poland., Wolberger C; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205, United States of America., Wolff M; ICS-6, Structural Biochemistry, Research Center Juelich, 52428, Juelich, Germany., Wright E; Biochemistry, Cell and Molecular Biology Department, University of Tennessee, Knoxville, Tennessee, 37996-0840, United States of America., Wu YS; Department of Chemical & Biomolecular Engineering, University of Delaware, Newark, Delaware, 19716, United States of America., Wubben JM; Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, 3086, Australia., Schuck P; Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, 20892, United States of America.

Publikováno v: PloS one [PLoS One] 2015 May 21; Vol. 10 (5), pp. e0126420. Date of Electronic Publication: 2015 May 21 (Print Publication: 2015).