Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Chase P Kelley"'
Autor:
Derek Cerchione, Katherine Loveluck, Eric L Tillotson, Fred Harbinski, Jen DaSilva, Chase P Kelley, Elise Keston-Smith, Cecilia A Fernandez, Vic E Myer, Hariharan Jayaram, Barrett E Steinberg
Publikováno v:
PLoS ONE, Vol 15, Iss 4, p e0231716 (2020)
RNA-guided endonucleases such as Cas9 provide efficient on-target genome editing in cells but may also cleave at off-target loci throughout the genome. Engineered variants of Streptococcus pyogenes Cas9 (SpCas9) have been developed to globally reduce
Externí odkaz:
https://doaj.org/article/2e8e195ece5248d19bf5391aebfa0fa0
Publikováno v:
Cell reports. 40(7)
Cas13 is a unique family of CRISPR endonucleases exhibiting programmable binding and cleavage of RNAs and is a strong candidate for eukaryotic RNA knockdown in the laboratory and the clinic. However, sequence-specific binding of Cas13 to the target R
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86f9559311294121a76f67df764eb505
https://pubmed.ncbi.nlm.nih.gov/35977479
https://pubmed.ncbi.nlm.nih.gov/35977479
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-19 (2021)
Nature Communications
Nature Communications
While the importance of RNA localization in highly differentiated cells is well appreciated, basic principles of RNA localization in skeletal muscle remain poorly characterized. Here, we develop a method to detect and quantify single molecule RNA loc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::396c62dd6971fecebd66e3c9c997d1b6
https://doi.org/10.1016/j.bpj.2021.11.533
https://doi.org/10.1016/j.bpj.2021.11.533
SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity
Autor:
Hariharan Jayaram, Derek Cerchione, Barrett Ethan Steinberg, Jen DaSilva, Elise Keston-Smith, Chase P. Kelley, Eric L. Tillotson, Fred Harbinski, Cecilia Fernández, Katherine Loveluck, Vic E. Myer
Publikováno v:
PLoS ONE
PLoS ONE, Vol 15, Iss 4, p e0231716 (2020)
PLoS ONE, Vol 15, Iss 4, p e0231716 (2020)
RNA-guided endonucleases such as Cas9 provide efficient on-target genome editing in cells but may also cleave at off-target loci throughout the genome. Engineered variants of Streptococcus pyogenes Cas9 (SpCas9) have been developed to globally reduce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c278f7fe6410c314896079cedeadd0e
https://pubmed.ncbi.nlm.nih.gov/32298334
https://pubmed.ncbi.nlm.nih.gov/32298334
Publikováno v:
Nature Reviews Molecular Cell Biology. 22:644-644
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f5c2c18d8c687c2970c393e9b2e0b552
https://doi.org/10.1038/s41580-021-00396-0
https://doi.org/10.1038/s41580-021-00396-0
Autor:
Jacob T. Barlow, Chase P. Kelley, Benjamin M. Geilich, Yunrong Chai, Thomas J. Webster, Srinivas Sridhar, Kevin Gozzi, Anne L. van de Ven
Publikováno v:
Applied Microbiology and Biotechnology. 101:455-464
Encapsulating bacteria within constrained microenvironments can promote the manifestation of specialized behaviors. Using double-emulsion droplet-generating microfluidic synthesis, live Bacillus subtilis bacteria were encapsulated in a semi-permeable
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9dc4f32f1a29660bed705afb20f84a38
https://doi.org/10.1007/s00253-016-7896-7
https://doi.org/10.1007/s00253-016-7896-7
Publikováno v:
Analytical chemistry. 86(6)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95fa344d4cfef2d5346485b5e85bf74e
https://pubmed.ncbi.nlm.nih.gov/24502446
https://pubmed.ncbi.nlm.nih.gov/24502446
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-19 (2021)
It is increasingly recognised that the spatial localisation of RNA is important for proper cellular function. Here, the authors investigate RNA localisation in skeletal muscle and develop methods to show that global active transport of RNA is require
Externí odkaz:
https://doaj.org/article/37f94ae6930f4c988885f815dfd8db0a