Zobrazeno 1 - 10
of 119
pro vyhledávání: '"Chase L, Beisel"'
Autor:
Chunlei Jiao, Natalia L. Peeck, Jiaqi Yu, Mohammad Ghaem Maghami, Sarah Kono, Daphne Collias, Sandra L. Martinez Diaz, Rachael Larose, Chase L. Beisel
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)
Abstract Many CRISPR-Cas immune systems generate guide (g)RNAs using trans-activating CRISPR RNAs (tracrRNAs). Recent work revealed that Cas9 tracrRNAs could be reprogrammed to convert any RNA-of-interest into a gRNA, linking the RNA’s presence to
Externí odkaz:
https://doaj.org/article/fae1f70b488348a591a8db8b7fd3dca3
Autor:
Aranzazu Arias-Rojas, Adini Q Arifah, Georgia Angelidou, Belal Alshaar, Ursula Schombel, Emma Forest, Dagmar Frahm, Volker Brinkmann, Nicole Paczia, Chase L Beisel, Nicolas Gisch, Igor Iatsenko
Publikováno v:
PLoS Pathogens, Vol 20, Iss 8, p e1012462 (2024)
Multiple peptide resistance factor (MprF) confers resistance to cationic antimicrobial peptides (AMPs) in several pathogens, thereby enabling evasion of the host immune response. The role of MprF in commensals remains, however, uncharacterized. To cl
Externí odkaz:
https://doaj.org/article/0c6cb570cd50400c90092644d5058a49
Autor:
Yanying Yu, Sandra Gawlitt, Lisa Barros de Andrade e Sousa, Erinc Merdivan, Marie Piraud, Chase L. Beisel, Lars Barquist
Publikováno v:
Genome Biology, Vol 25, Iss 1, Pp 1-22 (2024)
Abstract CRISPR interference (CRISPRi) is the leading technique to silence gene expression in bacteria; however, design rules remain poorly defined. We develop a best-in-class prediction algorithm for guide silencing efficiency by systematically inve
Externí odkaz:
https://doaj.org/article/b3fe9104d58c45dab45798f7f6947605
Publikováno v:
RNA Biology, Vol 20, Iss 1, Pp 666-680 (2023)
CRISPR technologies comprising a Cas nuclease and a guide RNA (gRNA) can utilize multiple gRNAs to enact multi-site editing or regulation in the same cell. Nature devised a highly compact means of encoding gRNAs in the form of CRISPR arrays composed
Externí odkaz:
https://doaj.org/article/fde7e1846cdc41ca814ee8c762767e35
Autor:
Ronay Cetin, Martin Wegner, Leah Luwisch, Sarada Saud, Tatjana Achmedov, Sebastian Süsser, Antonella Vera-Guapi, Konstantin Müller, Yves Matthess, Eva Quandt, Simone Schaubeck, Chase L. Beisel, Manuel Kaulich
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-16 (2023)
Abstract CRISPR-based gene perturbation enables unbiased investigations of single and combinatorial genotype-to-phenotype associations. In light of efforts to map combinatorial gene dependencies at scale, choosing an efficient and robust CRISPR-assoc
Externí odkaz:
https://doaj.org/article/0cb5e2ca754b4bf893f7228f118c97be
Autor:
Daphne Collias, Elena Vialetto, Jiaqi Yu, Khoa Co, Éva d. H. Almási, Ann-Sophie Rüttiger, Tatjana Achmedov, Till Strowig, Chase L. Beisel
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)
Genome editing in bacteria normally requires efficient recombination and high transformation efficiencies, which often isn’t. Here the authors report that systematically attenuating DNA targeting activity enables RecA-mediated repair in different b
Externí odkaz:
https://doaj.org/article/6847d05963c14cdda861862000397aca
Autor:
Chrysanthi Taxiarchi, Andrea Beaghton, Nayomi Illansinhage Don, Kyros Kyrou, Matthew Gribble, Dammy Shittu, Scott P. Collins, Chase L. Beisel, Roberto Galizi, Andrea Crisanti
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
Technologies that can halt the spread of gene drives would be highly useful in controlling or reverting their effect. Here the authors use the anti-CRISPR protein AcrIIA4 to inactivate drives in A. gambiae.
Externí odkaz:
https://doaj.org/article/d5874a5ae9a2467ba0f3206162213461
Autor:
Marialaura Gallo, Justin M. Vento, Pauline Joncour, Andrea Quagliariello, Elisa Maritan, Nuno F. Silva-Soares, Matteo Battistolli, Chase L. Beisel, Maria Elena Martino
Publikováno v:
iScience, Vol 25, Iss 6, Pp 104357- (2022)
Summary: Commensal bacteria are known to promote host growth. Such effect partly relies on the capacity of microbes to regulate the host’s transcriptional response. However, these evidences mainly come from comparing the transcriptional response ca
Externí odkaz:
https://doaj.org/article/ea2b77137fc04f34ba1b6fcd0055ac1e
Autor:
Anke Sparmann, Chase L Beisel
Publikováno v:
Molecular Systems Biology, Vol 18, Iss 4, Pp n/a-n/a (2022)
CRISPR‐Cas systems allow bacteria to memorize prior infections as a means to combat the same invader if it attempts another attack in the future. While the underlying mechanisms of this bacterial immunity have been intensely studied over the past d
Externí odkaz:
https://doaj.org/article/52dbc52592a3429db48f673ae4f0be69
Autor:
Daphne Collias, Chase L. Beisel
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
One of the key limitations of CRISPR-Cas-based genome editing techniques is the PAM dependency. Here, the authors review ongoing efforts towards realizing PAM-free nucleases, address potential consequences of eliminating PAM recognition, and propose
Externí odkaz:
https://doaj.org/article/3b0005fc19784851be7b5e4a5d35393a