Thermal Stabilisation of Lysozyme through Ensilication

Autor: Reveng A. Abdulkareem, Aswin Doekhie, Nikoletta Fotaki, Francoise Koumanov, Charlotte A. Dodson, Asel Sartbaeva

Publikováno v: Molecules, Vol 29, Iss 17, p 4207 (2024)

Protein therapeutics, vaccines, and other commercial products are often sensitive to environmental factors, such as temperature and long-term storage. In many cases, long-term protein stability is achieved by refrigeration or freezing. One alternativ

Externí odkaz: https://doaj.org/article/31720c39087649fa8c2630d59f027df1

A Chiral Lanthanide Tag for Stable and Rigid Attachment to Single Cysteine Residues in Proteins for NMR, EPR and Time‐Resolved Luminescence Studies

Autor: Gottfried Otting, Thomas R. Berki, Iresha D. Herath, Sarah H. Hewitt, Charlotte A. Dodson, Colum Breen, Nicholas Cox, Shereen Jabar, Stephen J. Butler, Martyna Judd, Ahmad F. Kassir

Publikováno v: Herath, I D, Breen, C, Hewitt, S H, Berki, T R, Kassir, A F, Dodson, C, Judd, M, Jabar, S, Cox, N, Otting, G & Butler, S J 2021, ' A chiral lanthanide tag for stable and rigid attachment to single cysteine residues in proteins for NMR, EPR and time-resolved luminescence studies ', Chemistry-A European Journal . https://doi.org/10.1002/chem.202101143
Chemistry (Weinheim an Der Bergstrasse, Germany)

A lanthanide‐binding tag site‐specifically attached to a protein presents a tool to probe the protein by multiple spectroscopic techniques, including nuclear magnetic resonance, electron paramagnetic resonance and time‐resolved luminescence spe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eca7463272775609da11a7debd7a4cd7
https://doi.org/10.1002/chem.202101143

A simple, robust, universal assay for real-time enzyme monitoring by signalling changes in nucleoside phosphate anion concentration using a europium(iii)-based anion receptor

Autor: Sarah H. Hewitt, Stephen J. Butler, Romain Mailhot, Rozee Ali, Chloe R Antonen, Charlotte A. Dodson

Publikováno v: Chemical Science

A simple, sensitive microplate assay for real-time enzyme monitoring, using a lanthanide-based anion receptor, could increase productivity in the drug discovery pipeline.
Enzymes that consume and produce nucleoside polyphosphate (NPP) anions rep

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31c7e0646f485be45422ca1620406fe7
https://pubmed.ncbi.nlm.nih.gov/31191895

Production of Protein Kinases in E. coli

Autor: Charlotte A. Dodson

Publikováno v: Methods in Molecular Biology ISBN: 9781493968855

Recombinant protein expression is widely used to generate milligram quantities of protein kinases for crystallographic, enzymatic, or other biophysical assays in vitro. Expression in E. coli is fast, cheap, and reliable. Here I present a detailed pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7e78ab468eba98ca3120b2be75d5c66e
https://doi.org/10.1007/978-1-4939-6887-9_16

Fluorescence-detected magnetic field effects on radical pair reactions from femtolitre volumes

Autor: Kiminori Maeda, Masaaki Murakami, Mark I. Wallace, P. J. Hore, Christopher J. Wedge, Charlotte A. Dodson

We show that the effects of applied magnetic fields on radical pair reactions can be sensitively measured from sample volumes as low as ∼100 femtolitres using total internal reflection fluorescence microscopy. Development of a fluorescence-based mi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f61a290a45ff76f34a4d86a05b7c490
https://doi.org/10.1039/c5cc01099c