Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Charles M. Romfo"'
Autor:
Jiyuan Ma, Thomas W. Davis, Young-Choon Moon, Hongyan Qi, Stuart W. Peltz, Janet Petruska, Neil Gregory Almstead, Nicole Risher, Art Branstrom, Jean Hedrick, Seongwoo Hwang, Katherine Cintron, William Joseph Lennox, Charles M. Romfo, Tamil Arasu, Liangxian Cao, Josephine Sheedy, John Babiak, Min Jung Kim, Ronald Kong, Wencheng Li, Jason D. Graci, Allan Jacobson, Shirley Yeh, Christopher R. Trotta, Joshua Du, Joseph M. Colacino, Bansri Furia, Marla Weetall
Publikováno v:
Molecular Cancer Therapeutics. 18:3-16
PTC299 was identified as an inhibitor of VEGFA mRNA translation in a phenotypic screen and evaluated in the clinic for treatment of solid tumors. To guide precision cancer treatment, we performed extensive biological characterization of the activity
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc21306fbe053e0ead2cdd503bd008ff
https://doi.org/10.1158/1535-7163.mct-18-0863
https://doi.org/10.1158/1535-7163.mct-18-0863
Publikováno v:
Genes & Development. 19:242-254
Pre-messenger RNA (pre-mRNA) splicing in all eukaryotes requires conserved intronic sequence elements located at the 5′- and 3′-splice sites and branchpoint for both accurate recognition of exon/intron boundaries and catalysis of the two transest
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bcd3edce7acb30729f236081acab655
https://doi.org/10.1101/gad.1265905
https://doi.org/10.1101/gad.1265905
Publikováno v:
RNA. 7:785-792
Trans-splicing requires that 5' and 3' splice sites be independently recognized. Here, we have used mutational analyses and a sensitive nuclease protection assay to determine the mechanism of trans-3' splice site recognition in vitro. Efficient recog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9864f4b6db1ece638bd2c063ba506be2
https://doi.org/10.1017/s1355838201010263
https://doi.org/10.1017/s1355838201010263
Publikováno v:
RNA. 6:1905-1909
This report describes a method that combines nuclease protection and site-specific labeling to determine sites and extents of RNA-protein interactions. The utility of the method is demonstrated by the analysis of the binding of factors to the 3' spli
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1ed02fc6ce94596533110b56ba010f9
https://doi.org/10.1017/s1355838200001308
https://doi.org/10.1017/s1355838200001308
Autor:
Willem J. van Heeckeren, Jo Ann Wise, Consuelo J. Alvarez, Charles M. Romfo, Christopher J. Webb
Publikováno v:
Molecular and Cellular Biology. 20:7955-7970
Schizosaccharomyces pombe pre-mRNAs are generally multi-intronic and share certain features with pre-mRNAs from Drosophila melanogaster, in which initial splice site pairing can occur via either exon or intron definition. Here, we present three lines
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba239e4921aa859aaaa875d899e3388d
https://doi.org/10.1128/mcb.20.21.7955-7970.2000
https://doi.org/10.1128/mcb.20.21.7955-7970.2000
Publikováno v:
Nature. 402:832-835
In metazoans, spliceosome assembly is initiated through recognition of the 5' splice site by U1 snRNP and the polypyrimidine tract by the U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor, U2AF. U2AF is a heterodimer comprising a l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e34c578a3796f0210a0e2e667fa39da7
https://doi.org/10.1038/45590
https://doi.org/10.1038/45590
Publikováno v:
Molecular biology of the cell. 16(2)
The heterodimeric splicing factor U2AF plays an important role in 3′ splice site selection, but the division of labor between the two subunits in vivo remains unclear. In vitro assays led to the proposal that the human large subunit recognizes 3′
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6445b75f2c7bc7b381ebc9abf1aafb9
https://pubmed.ncbi.nlm.nih.gov/15548596
https://pubmed.ncbi.nlm.nih.gov/15548596
Publikováno v:
Molecular cell. 6(2)
A sensitive assay based on competition between cis-and trans-splicing suggested that factors in addition to U1 snRNP were important for early 5' splice site recognition. Cross-linking and physical protection experiments revealed a functionally import
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4f053b4fd4fa51104f6812823534ba3
https://pubmed.ncbi.nlm.nih.gov/10983979
https://pubmed.ncbi.nlm.nih.gov/10983979
The large subunit of the mammalian U2AF heterodimer (U2AF 65 ) is essential for splicing in vitro. To expand our understanding of how this protein functions in vivo, we have created a null allele of the gene encoding the Schizosaccharomyces pombe ort
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29156b65f3c5ef37d47e46f2669211b8
https://europepmc.org/articles/PMC1369739/
https://europepmc.org/articles/PMC1369739/
Autor:
Jo Ann Wise, Charles M. Romfo
Publikováno v:
Nucleic acids research. 25(22)
While it is known that several trans -acting splicing factors are highly conserved between Schizosaccharomyces pombe and mammals, the roles of cis -acting signals have received comparatively little attention. In Saccharomyces cerevisiae, sequences do
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32c42b0b185981392c30a0979fd37e0f
https://pubmed.ncbi.nlm.nih.gov/9358179
https://pubmed.ncbi.nlm.nih.gov/9358179