Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Charles Lee Hershberger"'
Autor:
Jean-François Lefèvre, Laurence Baroche, Dominique Desplancq, Jose Michael Hanquier, Charles Lee Hershberger, Yannick Sorlet, Alain A. Vertès, Marc Ebtinger, Franc Pattus
Publikováno v:
Applied and Environmental Microbiology. 69:1108-1113
We produced bovine trypsinogen in the yeast Pichia pastoris . Little or no trypsinogen was detected when the gene with its native leader sequence was expressed under the control of the strong aox1 promoter, suggesting that expression of the wild-type
Autor:
Keith A. Brown, Joseph E. Tanner, Colleen A. Costello, Jeffrey Lynn Larson, Michael Andrew Menke, Robert Allen Payson, Charles Lee Hershberger, Raymond E. Kaiser, Milton J. Zmijewski
Publikováno v:
European Journal of Biochemistry. 267:5493-5501
A novel ketoreductase isolated from Zygosaccharomyces rouxii catalyzes the asymmetric reduction of selected ketone substrates of commercial importance. The 37.8-kDa ketoreductase was purified more than 300-fold to > 95% homogeneity from whole cells w
Autor:
Jane Larowe Sterner, Salvador Ventura, Jeffrey Lynn Larson, Josep Vendrell, Charles Lee Hershberger, Francesc X. Avilés, Virtudes Villegas
Publikováno v:
Journal of Biological Chemistry. 274:19925-19933
The proteolytic processing of pancreatic procarboxypeptidase B to a mature and functional enzyme is much faster than that of procarboxypeptidase A1. This different behavior has been proposed to depend on specific conformational features at the region
Autor:
Stanley Gene Burgett, Brigitte Elisabeth Schoner, R.Naaraja Rao, Martin Geistlich, Karen Cox, Eugene Thomas Seno, Charles Lee Hershberger, Paul Robert Rosteck, Patricia Ann Reynolds
Publikováno v:
Gene. 115:93-96
The three macrolide-resistance-encoding genes, tlrC from Streptomyces fradiae, srmB from Streptomyces ambofaciens, and carA from Streptomyces thermotolerans, encode proteins that possess significant sequence similarity to ATP-dependent transport prot
Autor:
M. W. Riemen, B. Arnold, Jeffrey Lynn Larson, P. Williams, P. A. Tice, P. Dunn, Paul Robert Rosteck, J. Ivancic, Charles Lee Hershberger, R. Crofts, K. L. O'neal, B. DeHOFF
Publikováno v:
Annals of the New York Academy of Sciences. 646:140-154
Autor:
Na N. Yang, Sushant Hardikar, Sheng-Ping Shiau, Charles Lee Hershberger, Andrew L. Glasebrook
Publikováno v:
Gene. 179(2)
The yeast URA3 gene was used as a reporter to investigate the activities of estrogenic and antiestrogenic compounds in yeast Saccharomyces cerevisiae. The control sequences of the wild type (wt) URA3 promoter were replaced with zero, two, or six copi
Autor:
Charles Lee Hershberger
Publikováno v:
Current opinion in biotechnology. 7(5)
Three elements came together during the past year to provide the opportunity to generate new polyketides. The first was the availability of cloned genes for several metabolic pathways; the second was a genetically defined host strain able to support
Publikováno v:
Gene. 102(1)
A tylosin(Ty)-producing strain of Streptomyces fradiae contains at least three genes, tlrA, tlrB, tlrC, specifying resistance to Ty (TyR). The complete nucleotide sequence of the TyR-encoding gene, tlrC, and the transcription start point of the gene
Autor:
Fishman Scott Eric, Eugene Thomas Seno, Jeffrey Lynn Larson, Karen Leigh Cox, Charles Lee Hershberger, Virginia Ann Birmingham
Publikováno v:
Molecular and General Genetics MGG. 204:532-539
A gene conferring high-level resistance to tylosin in Streptomyces lividans and Streptomyces griseofuscus was cloned from a tylosin-producing strain of Streptomyces fradiae. The tylosin-resistance (Tylr) gene (tlrA) was isolated on five overlapping D
Publikováno v:
Nucleic Acids Research. 3:2005-2018
Transfer RNA's were isolated from Euglena gracilis. Chloroplast cistrons for tRNA were quantitated by hybridizing tRNA to ct DNA. Species of tRNA hybridizing to ct DNA were partially purified by hybridization-chromatography. The tRNA's hybridizing to