Zobrazeno 1 - 10
of 69
pro vyhledávání: '"Charles L, Rutherford"'
Publikováno v:
genesis. 35:202-213
In Dictyostelium discoideum a phosphatase with a high pH optimum is known to increase in activity during cell differentiation and become localized to a narrow band of cells at the interface of prespore and prestalk cells. However, it was not clear if
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::521ebfae38b5d28d1b3901e4a4dd4ab5
https://doi.org/10.1002/gene.10185
https://doi.org/10.1002/gene.10185
Publikováno v:
Protein Expression and Purification. 27:375-383
A 1583 bp fragment of Dictyostelium alp cDNA (94% of the gene) was cloned in pET32a+. The enzyme was expressed in an inactive form in the inclusion body of the expression host BL21-CodonPlus (DE3)-RIL. The recombinant ALP constituted more than 50% of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d56765d0da7b79d5c788aa32398e4b7d
https://doi.org/10.1016/s1046-5928(02)00613-7
https://doi.org/10.1016/s1046-5928(02)00613-7
Publikováno v:
Molecular Carcinogenesis. 33:88-98
The LNCaP cell line is a versatile and useful model suitable for the study of human prostate cancer in vitro. It has been determined that the elevation of LNCaP intracellular cyclic adenosine monophosphate (cAMP) levels through the addition of membra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e0c3b8a71ecedf2690ac674ba0e1c531
https://doi.org/10.1002/mc.10025
https://doi.org/10.1002/mc.10025
Publikováno v:
Molecular Biology Reports. 29:301-316
Elevation of intracellular cAMP levels in the human prostatic adenocarcinoma cell line LNCaP results in the induction of reversible neuroendocrine differentiation and cell growth arrest. We have used the differential display technique to identify gen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c87f2910c91b2ac7be77cf4969031871
https://doi.org/10.1023/a:1020457400377
https://doi.org/10.1023/a:1020457400377
Autor:
Charles L. Rutherford, Nikita Warner
Publikováno v:
Archives of Biochemistry and Biophysics. 373:462-470
The glycogen phosphorylase-2 gene is developmentally regulated and plays a central role in cellular differentiation in Dictyostelium. There are two isozymes of glycogen phosphorylase, GP1 and GP2. Both forms are developmentally regulated; gp1 is expr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94846514179f3330aeae989dd5741fad
https://doi.org/10.1006/abbi.1999.1575
https://doi.org/10.1006/abbi.1999.1575
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1473:376-390
5'-Nucleotidase (5NU) in Dictyostelium discoideum is an enzyme that shows high substrate specificity to 5'-AMP. The enzyme has received considerable attention in the past because of the critical role played by cyclic AMP in cell differentiation in th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91a44daf981d5c0f6d1c4d4941c6ad07
https://doi.org/10.1016/s0304-4165(99)00206-8
https://doi.org/10.1016/s0304-4165(99)00206-8
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1351:111-125
The product of the glycogen phosphorylase-2 gene in Dictyostelium functions to provide the glucose units that are used to construct the structural components of the terminal stage of development. In this report, we link a 1233 bp upstream gp2 fragmen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d45ccba5e31eda2aa5f2e225e66f178d
https://doi.org/10.1016/s0167-4781(96)00182-0
https://doi.org/10.1016/s0167-4781(96)00182-0
Publikováno v:
Developmental Genetics. 19:350-364
We have partially purified the protein and isolated the glcS gene for glycogen synthase in Dictyostelium. glcS mRNA is present throughout development and is the product of a single gene coding for 775 amino acids, with a predicted molecular mass of 8
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8a2999e5f4e503369ceb4f3039093b80
https://doi.org/10.1002/(sici)1520-6408(1996)19:4<350::aid-dvg8>3.0.co
https://doi.org/10.1002/(sici)1520-6408(1996)19:4<350::aid-dvg8>3.0.co
Publikováno v:
Differentiation. 56:1-12
Glycogen phosphorylase 1 and 2, the isozymes responsible for glycogen degradation, are encoded by separate genes in Dictyostelium. The two gene products display different transcriptional and translational expression and distinct post-translational re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3bf2a9af60378ec685a092300373773
https://doi.org/10.1046/j.1432-0436.1994.56120001.x
https://doi.org/10.1046/j.1432-0436.1994.56120001.x
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1129:262-272
We have cloned cDNA and genomic DNA fragments from Dictyostelium discoiseum that represent the entire coding egion of glycogen phosphorylase 1 (gpl, α- d -glucosyltransferase, EC 2.4.1.1). Nucleic acid sequencing of the gp1 clones revealed a single
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3096bc49514fa488276268249900e7bf
https://doi.org/10.1016/0167-4781(92)90502-q
https://doi.org/10.1016/0167-4781(92)90502-q