Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Charles F. Earhart"'
Autor:
Charles F. Earhart, Feras M. Hantash
Publikováno v:
Journal of Bacteriology. 182:1768-1773
The cytosolic proteins EntE, EntF, and EntB/G, which are Escherichia coli enzymes necessary for the final stage of enterobactin synthesis, are released by osmotic shock. Here, consistent with the idea that cytoplasmic proteins found in shockates have
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75c15710fa179923f61e39e41360f02c
https://doi.org/10.1128/jb.182.6.1768-1773.2000
https://doi.org/10.1128/jb.182.6.1768-1773.2000
Publikováno v:
Applied Microbiology and Biotechnology. 45:112-119
The Lpp'OmpA(46-159) hybrid protein can serve as an efficient targeting vehicle for localizing a variety of procaryotic and eucaryotic soluble proteins onto the E. coli surface, thus providing a system for several possible biotechnology applications.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d483c2148ee082aa46763857dd669d77
https://doi.org/10.1007/s002530050657
https://doi.org/10.1007/s002530050657
Autor:
George Georgiou, Christos Stathopoulos, Daren L. Stephens, Heather L. Poetschke, John Mendenhall, Charles F. Earhart
Publikováno v:
"Protein Engineering, Design and Selection". 9:239-247
Bacterial cell-surface exposure of foreign peptides and soluble proteins has been achieved recently by employing a fusion protein methodology. An Lpp'-OmpA(46-159)-Bla fusion protein has been shown previously to display the normally periplasmic enzym
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83214dde1985fec29e97751f557f7c4f
https://doi.org/10.1093/protein/9.2.239
https://doi.org/10.1093/protein/9.2.239
Autor:
J F Staab, Charles F. Earhart
Publikováno v:
Journal of Bacteriology. 172:6403-6410
The last steps in the biosynthesis of the Escherichia coli siderophore enterobactin (Ent) are carried out by Ent synthetase, a multienzyme complex believed to be composed of the entD, -E, -F, and -G products (EntD to -G). However, sequencing data sho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::287d4e690cb8d59d28331543fade102b
https://doi.org/10.1128/jb.172.11.6403-6410.1990
https://doi.org/10.1128/jb.172.11.6403-6410.1990
Autor:
Charles F. Earhart
Publisher Summary This chapter discusses the application of lipoprotein (Lpp)–OmpA fusion vehicle to study the surface of bacteria. The Lpp–OmpA vector is designed for use in the gram-negative bacterium Escherichia coli ; the rationale for its di
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::97586ddfc4f91b103d2bacee17dc4eb4
https://doi.org/10.1016/s0076-6879(00)26072-2
https://doi.org/10.1016/s0076-6879(00)26072-2
Publikováno v:
Microbiology (Reading, England). 143
The terminal reactions in the synthesis of the siderophore enterobactin (Ent) by Escherichia coli require the EntD, E, F and B/G polypeptides. The idea that these molecules form a complex (Ent synthase) that is membrane-associated was re-evaluated. I
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c41f5ee1e502061954656d98271869e3
https://pubmed.ncbi.nlm.nih.gov/9025288
https://pubmed.ncbi.nlm.nih.gov/9025288
Publikováno v:
Microbiology (Reading, England). 141
Summary: Most high-affinity systems for iron uptake in Gram-negative bacteria are thought to employ periplasmic-binding-protein-dependent transport. In Escherichia coli, FepB is a periplasmic protein required for uptake of iron complexed to its endog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d808f245c600207d032040300ee5b620
https://pubmed.ncbi.nlm.nih.gov/7551033
https://pubmed.ncbi.nlm.nih.gov/7551033
Autor:
Charles F. Earhart, Sara S. Chenault
Publikováno v:
Journal of general microbiology. 138(10)
In Escherichia coli, iron assimilation by means of the siderophore enterobactin requires two hydrophobic cytoplasmic membrane proteins, FepD and FepG, which are essential components of a binding-protein-dependent transport system. Such components are
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3268ab6b04a6ec62ebc1364ce3f00ce0
https://pubmed.ncbi.nlm.nih.gov/1479347
https://pubmed.ncbi.nlm.nih.gov/1479347
Autor:
Charles F. Earhart
Publikováno v:
Biology of Metals. 4:70-71
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::791a76db44ac280edbaa08b6d58132ae
https://doi.org/10.1007/bf01135560
https://doi.org/10.1007/bf01135560
Autor:
M.D. Lundrigan, Charles F. Earhart
Publikováno v:
FEMS Microbiology Letters. 24:341-344
The outer membrane protein BtuB of Escherichia coli K-12 is the receptor for vitamin B12; it is normally present in approx. 200 copies per cell. We describe here the conditions by which BtuB was readily observed in electropherograms of outer membrane
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::71cf3864c5967e2adfae14bdfede1dff
https://doi.org/10.1111/j.1574-6968.1984.tb01331.x
https://doi.org/10.1111/j.1574-6968.1984.tb01331.x