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pro vyhledávání: '"Charles E. Helmstetter"'
Autor:
Charles E. Helmstetter
Publikováno v:
Life, Vol 13, Iss 4, p 977 (2023)
The basic properties of the Escherichia coli duplication process can be defined by two time periods: C, the time for a round of chromosome replication, and D, the time between the end of a round of replication and cell division. Given the durations o
Externí odkaz:
https://doaj.org/article/f2a8688105804e5885ba36aabc46e75b
Publikováno v:
BioTechniques, Vol 32, Iss 5, Pp 1098-1105 (2002)
A method is described for producing sizable quantities of synchronously dividing, minimally disturbed mammalian cells. Cultures were grown immobilized on surfaces such that cell division within the population resulted in the continuous release of syn
Externí odkaz:
https://doaj.org/article/bd1c63c8794941fcba360de0617fbe03
Autor:
Arieh Zaritsky, Conrad L. Woldringh, Norbert O. E. Vischer, Ping Wang, Charles E. Helmstetter
Publikováno v:
Journal of Systemics, Cybernetics and Informatics, Vol 10, Iss 1, Pp 18-23 (2012)
The bacterial Cell Cycle is presented using the Simulation program CCSim, which employs four parameters related to time (inter-division τ, replication C, division D) and size (mass at replication initiation Mi), sufficient to describe and compare ba
Autor:
Charles E. Helmstetter, Ping Wang, Norbert O. E. Vischer, Conrad L. Woldringh, Arieh Zaritsky
Publikováno v:
Journal of Systemics, Cybernetics and Informatics, Vol 10, Iss 1, Pp 18-23 (2012)
The bacterial Cell Cycle is presented using the Simulation program CCSim, which employs four parameters related to time (inter-division τ, replication C, division D) and size (mass at replication initiation Mi), sufficient to describe and compare ba
Autor:
Charles E. Helmstetter
Publikováno v:
Frontiers in Microbiology
Frontiers in Microbiology, Vol 6 (2015)
Frontiers in Microbiology, Vol 6 (2015)
My effort to use synchronously dividing cultures to examine the Escherichia coli cell cycle involved a ten-year struggle with failure after failure punctuated by a few gratifying successes, especially at the end. In this essay, I recount my personal
Publikováno v:
Cytotechnology. 51:149-157
A culture system for performing cell cycle analyses on cells in undisturbed steady-state populations was designed and tested. In this system, newborn cells are shed continuously from an immobilized, perfused culture rotating about the horizontal axis
Publikováno v:
Cell Cycle. 3:1055-1059
The cyclins are tightly regulated elements governing eukaryotic cell cycle progression by means of sequential activation-inactivation of cyclin-dependent kinases. In one manifestation of this regulation, the mRNA levels of several cyclin genes oscill
Publikováno v:
Cell Cycle. 2:42-45
Growth characteristics of synchronous human MOLT-4, human U-937 and mouse L1210 cultures produced with a new minimally-disturbing technology were compared to each other and to synchronous Escherichia coli B/r. Based on measurements of cell concentrat
Publikováno v:
Journal of Basic Microbiology. 39:237-242
In Escherichia coli, the genes of the main heat-shock proteins are under the control of the product of gene rpoH, protein sigma 32. The distal promoter P1 of rpoH is located within the terminator of the division operon ftsYEX which could imply some c
Autor:
Charles E. Helmstetter
Publikováno v:
Proceedings of the National Academy of Sciences. 94:10195-10198
A novel culture system for mammalian cells was used to investigate division orientations in populations of Chinese hamster ovary cells and the influence of gravity on the positioning of division axes. The cells were tethered to adhesive sites, smalle