Zobrazeno 1 - 10
of 87
pro vyhledávání: '"Charles A. Brearley"'
Autor:
Joseph J. Carroll, Colleen Sprigg, Graham Chilvers, Ignacio Delso, Megan Barker, Filipa Cox, David Johnson, Charles A. Brearley
Publikováno v:
Methods in Ecology and Evolution, Vol 15, Iss 3, Pp 530-543 (2024)
Abstract Organic forms of phosphorus (P) prevail in soils and their quantification is vital to better understand global biogeochemical cycles. P speciation in soil is commonly assessed by 31P NMR spectroscopy of sodium hydroxide‐EDTA (NaOH‐EDTA)
Externí odkaz:
https://doaj.org/article/4a0d7bbde42e492c9df289fd2ca9c52d
Autor:
María Ángeles Márquez-Moñino, Raquel Ortega-García, Hayley Whitfield, Andrew M. Riley, Lourdes Infantes, Shane W. Garrett, Megan L. Shipton, Charles A. Brearley, Barry V. L. Potter, Beatriz González
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-16 (2024)
Abstract d-myo-inositol 1,4,5-trisphosphate (InsP3) is a fundamental second messenger in cellular Ca2+ mobilization. InsP3 3-kinase, a highly specific enzyme binding InsP3 in just one mode, phosphorylates InsP3 specifically at its secondary 3-hydroxy
Externí odkaz:
https://doaj.org/article/2e9f8695a0ad4929bc5af1359e3b8bdc
Autor:
Colleen Sprigg, Philip T. Leftwich, Emily Burton, Dawn Scholey, Michael R. Bedford, Charles A. Brearley
Publikováno v:
PLoS ONE, Vol 18, Iss 6 (2023)
Inert digestibility index markers such as titanium dioxide are universally accepted to provide simple measurement of digestive tract retention and relative digestibility in poultry feeding trials. Their use underpins industry practice: specifically d
Externí odkaz:
https://doaj.org/article/3042babdd3a64c64bda49c5f2d2d5dad
Publikováno v:
Microbial Biotechnology, Vol 14, Iss 4, Pp 1409-1421 (2021)
Summary HPLC methods are shown to be of predictive value for classification of phytase activity of aggregate microbial communities and pure cultures. Applied in initial screens, they obviate the problems of ‘false‐positive’ detection arising fr
Externí odkaz:
https://doaj.org/article/a9f1f640c12640309d47f76e4e972cc1
Autor:
Colleen Sprigg, Hayley Whitfield, Emily Burton, Dawn Scholey, Michael R. Bedford, Charles A. Brearley
Publikováno v:
PLoS ONE, Vol 17, Iss 10 (2022)
Phytases, enzymes that degrade phytate present in feedstuffs, are widely added to the diets of monogastric animals. Many studies have correlated phytase addition with improved animal productivity and a subset of these have sought to correlate animal
Externí odkaz:
https://doaj.org/article/0867b1f8f90f4378a4b02140ba5f8e24
Autor:
Isabella M. Acquistapace, Emma J. Thompson, Imke Kühn, Mike R. Bedford, Charles A. Brearley, Andrew M. Hemmings
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 11, p 6346 (2022)
AppA, the Escherichia coli periplasmic phytase of clade 2 of the histidine phosphatase (HP2) family, has been well-characterized and successfully engineered for use as an animal feed supplement. AppA is a 1D-6-phytase and highly stereospecific but tr
Externí odkaz:
https://doaj.org/article/47519e02733b4063b6abc2fdfe939c03
Autor:
Régis Stentz, Samantha Osborne, Nikki Horn, Arthur W.H. Li, Isabelle Hautefort, Roy Bongaerts, Marine Rouyer, Paul Bailey, Stephen B. Shears, Andrew M. Hemmings, Charles A. Brearley, Simon R. Carding
Publikováno v:
Cell Reports, Vol 6, Iss 4, Pp 646-656 (2014)
Dietary InsP6 can modulate eukaryotic cell proliferation and has complex nutritive consequences, but its metabolism in the mammalian gastrointestinal tract is poorly understood. Therefore, we performed phylogenetic analyses of the gastrointestinal mi
Externí odkaz:
https://doaj.org/article/6f07e18ff31c45b3ac924520daba24da
Autor:
Gregory D Rix, Colleen Sprigg, Hayley Whitfield, Andrew M Hemmings, Jonathan D Todd, Charles A Brearley
Publikováno v:
PLoS ONE, Vol 17, Iss 8, p e0272015 (2022)
Phylogenetic analysis, homology modelling and biochemical methods have been employed to characterize a phytase from a Gram-negative soil bacterium. Acinetobacter sp. AC1-2 phytase belongs to clade 2 of the histidine (acid) phytases, to the Multiple I
Externí odkaz:
https://doaj.org/article/075e0e1afcee4c929575be7980802230
Autor:
Hayley L. Whitfield, Sining He, Yinghong Gu, Colleen Sprigg, Hui-Fen Kuo, Tzyy-Jen Chiou, Andrew M. Riley, Barry V.L. Potter, Andrew M. Hemmings, Charles A. Brearley
Myo-inositol tris/tetrakisphosphate kinases (ITPKs) catalyze diverse phosphotransfer reactions with myo-inositol phosphate and myo-inositol pyrophosphate substrates. However, the lack of structures of nucleotide-coordinated plant ITPKs thwarts a rati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11707867046f2bd631713c3e5e76a49b
https://ueaeprints.uea.ac.uk/id/eprint/91695/
https://ueaeprints.uea.ac.uk/id/eprint/91695/
Autor:
Isabella M. Acquistapace, Arthur W.H. Li, Melissa Salmon, Michael R. Bedford, Imke Kühn, Charles A. Brearley, Andrew M. Hemmings, Monika A. Zi¸etek
Publikováno v:
The Journal of Biological Chemistry
Highly engineered phytases, which sequentially hydrolyze the hexakisphosphate ester of inositol known as phytic acid, are routinely added to the feeds of monogastric animals to improve phosphate bioavailability. New phytases are sought as starting po