Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Charelle Boot"'
Autor:
Adriana Martinez-Silgado, Fjodor A. Yousef Yengej, Jens Puschhof, Veerle Geurts, Charelle Boot, Maarten H. Geurts, Maarten B. Rookmaaker, Marianne C. Verhaar, Joep Beumer, Hans Clevers
Publikováno v:
STAR Protocols, Vol 3, Iss 3, Pp 101639- (2022)
Summary: Intestinal organoids are three-dimensional cultures that resemble key aspects of the epithelium of origin. Here, we describe how to differentiate human small intestinal organoids by combining growth media variations and genetic engineering.
Externí odkaz:
https://doaj.org/article/dcf1297473074b90b8864176f730bcd6
Autor:
Jawara Allen, Axel Rosendahl Huber, Cayetano Pleguezuelos-Manzano, Jens Puschhof, Shaoguang Wu, Xinqun Wu, Charelle Boot, Aurelia Saftien, Heather M. O’Hagan, Hao Wang, Ruben van Boxtel, Hans Clevers, Cynthia L. Sears
Publikováno v:
Microbiology Spectrum, Vol 10, Iss 3 (2022)
ABSTRACT Enterotoxigenic Bacteroides fragilis (ETBF) is consistently found at higher frequency in individuals with sporadic and hereditary colorectal cancer (CRC) and induces tumorigenesis in several mouse models of CRC. However, whether specific mut
Externí odkaz:
https://doaj.org/article/c8b925b4810c4dcabf9cb25d68e6536b
Autor:
Rachel Tanner, Andrew D. White, Charelle Boot, Claudia C. Sombroek, Matthew K. O’Shea, Daniel Wright, Emily Hoogkamer, Julia Bitencourt, Stephanie A. Harris, Charlotte Sarfas, Rachel Wittenberg, Iman Satti, Helen A. Fletcher, Frank A. W. Verreck, Sally A. Sharpe, Helen McShane
Publikováno v:
npj Vaccines, Vol 6, Iss 1, Pp 1-11 (2021)
Abstract We present a non-human primate mycobacterial growth inhibition assay (MGIA) using in vitro blood or cell co-culture with the aim of refining and expediting early tuberculosis vaccine testing. We have taken steps to optimise the assay using c
Externí odkaz:
https://doaj.org/article/68af0e0e83b24984a10595f180e34dba
Autor:
Rachel Tanner, Emily Hoogkamer, Julia Bitencourt, Andrew White, Charelle Boot, Claudia C. Sombroek, Stephanie A. Harris, Matthew K. O'Shea, Daniel Wright, Rachel Wittenberg, Charlotte Sarfas, Iman Satti, Frank A.W. Verreck, Sally A. Sharpe, Helen A. Fletcher, Helen McShane
Publikováno v:
F1000Research, Vol 10 (2021)
The only currently available approach to early efficacy testing of tuberculosis (TB) vaccine candidates is in vivo preclinical challenge models. These typically include mice, guinea pigs and non-human primates (NHPs), which must be exposed to virulen
Externí odkaz:
https://doaj.org/article/f4d160f312314877839761de896f12a2
Autor:
Rachel Tanner, Emily Hoogkamer, Julia Bitencourt, Andrew White, Charelle Boot, Claudia C. Sombroek, Stephanie A. Harris, Matthew K. O'Shea, Daniel Wright, Rachel Wittenberg, Charlotte Sarfas, Iman Satti, Frank A.W. Verreck, Sally A. Sharpe, Helen A. Fletcher, Helen McShane
Publikováno v:
F1000Research, Vol 10 (2021)
The only currently available approach to early efficacy testing of tuberculosis (TB) vaccine candidates is in vivo preclinical challenge models. These typically include mice, guinea pigs and non-human primates (NHPs), which must be exposed to virulen
Externí odkaz:
https://doaj.org/article/8d457b8383a642419000e0f51934d0e4
Autor:
Karin Dijkman, Richard A. W. Vervenne, Claudia C. Sombroek, Charelle Boot, Sam O. Hofman, Krista E. van Meijgaarden, Tom H. M. Ottenhoff, Clemens H. M. Kocken, Krista G. Haanstra, Michel P. M. Vierboom, Frank A. W. Verreck
Publikováno v:
Frontiers in Immunology, Vol 10 (2019)
While tuberculosis continues to afflict mankind, the immunological mechanisms underlying TB disease development are still incompletely understood. Advanced preclinical models for TB research include both rhesus and cynomolgus macaques (Macaca mulatta
Externí odkaz:
https://doaj.org/article/60dd21741d194b77915749c68bee85a6
Autor:
Inha Heo, Hans Clevers, Ninouk Akkerman, Adriana Martinez-Silgado, Aurelia Saftien, Cayetano Pleguezuelos-Manzano, Charelle Boot, Joep Beumer, Amy de Waal, Devanjali Dutta, Jens Puschhof
Publikováno v:
Nature Protocols. 16(10):4633-4649
Adult-stem-cell-derived organoids model human epithelial tissues ex vivo, which enables the study of host–microbe interactions with great experimental control. This protocol comprises methods to coculture organoids with microbes, particularly focus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::418e4d13fe69c8463d29d7970bf1dda6
https://doi.org/10.1038/s41596-021-00589-z
https://doi.org/10.1038/s41596-021-00589-z
Autor:
Joep Beumer, Julia Bauzá-Martinez, Tim S. Veth, Veerle Geurts, Charelle Boot, Hannah Gilliam-Vigh, Steen S. Poulsen, Filip K. Knop, Wei Wu, Hans Clevers
Publikováno v:
Beumer, J, Bauzá-Martinez, J, Veth, T S, Geurts, V, Boot, C, Gilliam-Vigh, H, Poulsen, S S, Knop, F K, Wu, W & Clevers, H 2022, ' Mapping prohormone processing by proteases in human enteroendocrine cells using genetically engineered organoid models ', Proceedings of the National Academy of Sciences of the United States of America, vol. 119, no. 46, e2212057119 . https://doi.org/10.1073/pnas.2212057119
Proceedings of the National Academy of Sciences of the United States of America, 119(46). National Academy of Sciences
Proceedings of the National Academy of Sciences of the United States of America, 119(46). National Academy of Sciences
Enteroendocrine cells (EECs) secrete hormones in response to ingested nutrients to control physiological processes such as appetite and insulin release. EEC hormones are synthesized as large proproteins that undergo proteolytic processing to generate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::266b5fb4e15516660a7b245ba371ce25
https://pubmed.ncbi.nlm.nih.gov/36343264
https://pubmed.ncbi.nlm.nih.gov/36343264
Autor:
Rachel, Tanner, Emily, Hoogkamer, Julia, Bitencourt, Andrew, White, Charelle, Boot, Claudia C, Sombroek, Stephanie A, Harris, Matthew K, O'Shea, Daniel, Wright, Rachel, Wittenberg, Charlotte, Sarfas, Iman, Satti, Frank A W, Verreck, Sally A, Sharpe, Helen A, Fletcher, Helen, McShane
Publikováno v:
F1000Research
The only currently available approach to early efficacy testing of tuberculosis (TB) vaccine candidates is in vivo preclinical challenge models. These typically include mice, guinea pigs and non-human primates (NHPs), which must be exposed to virulen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2d5d6e84b1ac06b4dd26fe60af550d8b
https://pubmed.ncbi.nlm.nih.gov/33976866
https://pubmed.ncbi.nlm.nih.gov/33976866
Autor:
E Hoogkamer, Andrew White, Helen A. Fletcher, Daniel B. Wright, Verreck Faw., Stephanie A. Harris, Charlotte Sarfas, Claudia C. Sombroek, J Bitencourt, Charelle Boot, Rachel Tanner, Sally Sharpe, Helen McShane, Matthew K. O'Shea, Iman Satti, Rachel Wittenberg
Publikováno v:
npj Vaccines, Vol 6, Iss 1, Pp 1-11 (2021)
NPJ Vaccines
NPJ Vaccines
We present a non-human primate mycobacterial growth inhibition assay (MGIA) using in vitro blood or cell co-culture with the aim of refining and expediting early tuberculosis vaccine testing. We have taken steps to optimise the assay using cryopreser
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::782fb2ef066acb216357698e58030129
https://doi.org/10.1038/s41541-020-00263-7
https://doi.org/10.1038/s41541-020-00263-7