Zobrazeno 1 - 10
of 46
pro vyhledávání: '"Chaoyou Xue"'
Publikováno v:
Cell Reports, Vol 43, Iss 5, Pp 114225- (2024)
Summary: Protospacer-adjacent motif (PAM) recognition licenses Cas nucleases for genome engineering applications, thereby restricting gene targeting to PAM-containing regions. Protein engineering has led to PAM-relaxed SpCas9 variants like SpG and Sp
Externí odkaz:
https://doaj.org/article/e99fc66d99734c8491b6715ec5329ba6
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 35, Iss 1, Pp 102138- (2024)
Programmable genome insertion (or knock-in) is vital for both fundamental and translational research. The continuously expanding number of CRISPR-based genome insertion strategies demonstrates the ongoing development in this field. Common methods for
Externí odkaz:
https://doaj.org/article/828f5721e0e64867a6a78a6056125f68
Autor:
Chaoyou Xue, Sameer J. Salunkhe, Nozomi Tomimatsu, Ajinkya S. Kawale, Youngho Kwon, Sandeep Burma, Patrick Sung, Eric C. Greene
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022)
Bloom syndrome is a genetic disorder associated with increased cancer risk and is caused by mutations in Bloom helicase. This study investigates the mechanisms used by BLM helicase as initiates the repair of broken chromosomes.
Externí odkaz:
https://doaj.org/article/249006cafba94dcf911c7aad8b796856
Publikováno v:
Cell Reports, Vol 21, Iss 13, Pp 3717-3727 (2017)
CRISPR-Cas systems defend bacteria and archaea against infection by bacteriophage and other threats. The central component of these systems are surveillance complexes that use guide RNAs to bind specific regions of foreign nucleic acids, marking them
Externí odkaz:
https://doaj.org/article/4e7608405aa1403f89895335b391f4b2
Publikováno v:
Metabolic Engineering. 67:133-144
Stoichiometric genome-scale metabolic network models (GEMs) have been widely used to predict metabolic phenotypes. In addition to stoichiometric ratios, other constraints such as enzyme availability and thermodynamic feasibility can also limit the ph
Autor:
Weixing Zhao, Lucia Molnarova, Chaoyou Xue, Mário Špírek, Ondrej Beláň, Kyle Kaniecki, Katerina Krejci, Lumir Krejci, Patrick Sung, Eric C. Greene, Simon J. Boulton, Justin B. Steinfeld, Chujian Ma, Youngho Kwon
Publikováno v:
Nucleic Acids Research
RECQ5 is one of five RecQ helicases found in humans and is thought to participate in homologous DNA recombination by acting as a negative regulator of the recombinase protein RAD51. Here, we use kinetic and single molecule imaging methods to monitor
Autor:
Ning Jia, Mihaela C. Unciuleac, Chaoyou Xue, Eric C. Greene, Dinshaw J. Patel, Stewart Shuman
Publikováno v:
Proc Natl Acad Sci U S A
Mycobacterial AdnAB is a heterodimeric helicase–nuclease that initiates homologous recombination by resecting DNA double-strand breaks (DSBs). The AdnA and AdnB subunits are each composed of an N-terminal motor domain and a C-terminal nuclease doma
Autor:
Eric C. Greene, James M. Daley, Xiaoyu Xue, Youngho Kwon, Chaoyou Xue, Patrick Sung, Justin B. Steinfeld
Publikováno v:
Nucleic Acids Research
Bloom helicase (BLM) and its orthologs are essential for the maintenance of genome integrity. BLM defects represent the underlying cause of Bloom Syndrome, a rare genetic disorder that is marked by strong cancer predisposition. BLM deficient cells ac
Publikováno v:
Nucleic Acids Research
DNA helicases of the RecQ family are conserved among the three domains of life and play essential roles in genome maintenance. Mutations in several human RecQ helicases lead to diseases that are marked by cancer predisposition. The Saccharomyces cere
Autor:
Chaoyou Xue, Eric C. Greene, Mihaela-Carmen Unciuleac, Aviv Meir, Stewart Shuman, Garrett M. Warren
Publikováno v:
Proc Natl Acad Sci U S A
Mycobacterial AdnAB is a heterodimeric helicase-nuclease that initiates homologous recombination by resecting DNA double-strand breaks (DSBs). The N-terminal motor domain of the AdnB subunit hydrolyzes ATP to drive rapid and processive 3' to 5' trans
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::868f151c54ca333a291190be5e31836d
https://europepmc.org/articles/PMC7980473/
https://europepmc.org/articles/PMC7980473/