Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Changzeng Zhao"'
Publikováno v:
Plant Direct, Vol 4, Iss 3, Pp n/a-n/a (2020)
Abstract Site‐specific recombinase enzymes function in heterologous cellular environments to initiate strand‐switching reactions between unique DNA sequences termed recombinase binding sites. Depending on binding site position and orientation, re
Externí odkaz:
https://doaj.org/article/98250771f54b48d79e75bc3faadca90f
Autor:
Jon P. Cody, Nathan C. Swyers, Morgan E. McCaw, Nathaniel D. Graham, Changzeng Zhao, James A. Birchler
Publikováno v:
Agronomy, Vol 5, Iss 3, Pp 309-321 (2015)
Minichromosome technology has the potential to offer a number of possibilities for expanding current biofortification strategies. While conventional genome manipulations rely on random integration of one or a few genes, engineered minichromosomes wou
Externí odkaz:
https://doaj.org/article/bf928fdc086a4d8eb2685a0e73e6b8bd
Publikováno v:
Bio-Protocol, Vol 5, Iss 18 (2015)
Minichromosomes are small, autonomously functioning chromosomes that exist separately from the normal chromosomal set. Creation of minichromosomes in plants relies on telomere truncation to remove the chromosome arms and the native telomere sequence
Externí odkaz:
https://doaj.org/article/0286970f4f81407584c9f8be393a36ae
Autor:
Jan Vrána, Gil Ben-Zvi, Pavel Solanský, Jonathan C. Lamb, Yalin Liu, Lucie Šimková, Jie Hou, Fangpu Han, Zhi Gao, Ryan N. Douglas, Jianlin Cheng, Jinghua Shi, Xiaowen Shi, R. Kelly Dawe, Mohamed El-Walid, Kobi Baruch, Lior Glick, Handong Su, James A. Birchler, Jaroslav Doležel, Hua Yang, Chen Chen, Miroslava Karafiátová, Jing Zhang, Patrice S. Albert, Changzeng Zhao, Nicolas Blavet, Kathleen J. Newton, Tieming Ji, Jan Bartoš, Guy Kol, Hana Šimková, Morgan E. McCaw
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America
Significance B chromosomes are nonvital chromosomes found in thousands of plants and animals that persist through various drive mechanisms. The drive mechanism of the maize B chromosome consists of mitotic nondisjunction at the second pollen division
Autor:
Nathan C. Swyers, Morgan E. McCaw, James A. Birchler, Jon P. Cody, Changzeng Zhao, Nathaniel D. Graham
Publikováno v:
Current Protocols in Plant Biology
Fluorescence In Situ Hybridization (FISH) is the annealing of fluorescent DNA probes to their complementary sequences on prepared chromosomes and subsequent visualization with a fluorescent microscope. In maize, FISH is useful for distinguishing each
Autor:
Nathan C. Swyers, Jon P. Cody, James A. Birchler, Morgan E. McCaw, Nathaniel D. Graham, Changzeng Zhao
Publikováno v:
Current Protocols in Plant Biology
High-quality preparations of chromosomes are useful for many purposes. To prepare metaphase chromosome spreads in maize, root tips are harvested and treated with nitrous oxide to stop cell division at metaphase before being fixed in acetic acid. This
Autor:
Jon P. Cody, Robert T. Gaeta, Morgan E. McCaw, Nathan C. Swyers, Changzeng Zhao, James A. Birchler, Nathaniel D. Graham
Publikováno v:
Current Protocols in Plant Biology
Minichromosomes have been generated in maize using telomere-mediated truncation. Telomere DNA, because of its repetitive nature, can be difficult to manipulate. The protocols in this unit describe two methods for generating the telomere DNA required
Autor:
Blavet, Nicolas, Hua Yang, Handong Su, Solanský, Pavel, Douglas, Ryan N., Karafiátová, Miroslava, Šimková, Lucie, Jing Zhang, Yalin Liu, Jie Hou, Xiaowen Shi, Chen Chen, El-Walid, Mohamed, McCaw, Morgan E., Albert, Patrice S., Zhi Gao, Changzeng Zhao, Gil Ben-Zvi, Lior Glick, Guy Kol
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America; 6/8/2021, Vol. 118 Issue 23, p1-11, 11p
Autor:
Changzeng Zhao, Rick E. Masonbrink, Robert T. Gaeta, James A. Birchler, Abhijit Sanyal, Lakshminarasimhan Krishnaswamy
Publikováno v:
Chromosoma. 122:221-232
Engineered minichromosomes provide efficient platforms for stacking transgenes in crop plants. Methods for modifying these chromosomes in vivo are essential for the development of customizable systems for the removal of selection genes or other seque
Autor:
Nathaniel, Graham, Nathan, Swyers, Jon, Cody, Morgan, McCaw, Changzeng, Zhao, James A, Birchler
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1469
Artificial minichromosomes are non-integrating vectors capable of stably maintaining transgenes outside of the main chromosome set. The production of minichromosomes relies on telomere-mediated chromosomal truncation, which involves introducing trans