Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Changchang Xin"'
Autor:
Jianhang Yin, Kailun Fang, Yanxia Gao, Liqiong Ou, Shaopeng Yuan, Changchang Xin, Weiwei Wu, Wei-wei Wu, Jiaxu Hong, Hui Yang, Jiazhi Hu
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-8 (2022)
Undesired chromosomal translocations, vector integrations, and large deletions remain a problem for therapeutic gene editing in vivo. Here, the authors compare the CRISPR-Cas9TX variant with CRISPR-Cas9 and show elimination of chromosomal translocati
Externí odkaz:
https://doaj.org/article/ead978410b604a268f507033d862566f
Autor:
Changchang Xin, Jianhang Yin, Shaopeng Yuan, Liqiong Ou, Mengzhu Liu, Weiwei Zhang, Jiazhi Hu
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
CRISPR-Cas12f nucleases can be effectively packaged into AAVs for gene therapy, but a systematic evaluation of editing outcomes is lacking. Here the authors perform a comprehensive assessment of 4 Cas12f proteins and compare to Cas9 and two Cas12a pr
Externí odkaz:
https://doaj.org/article/332dbab4ca354529bd6a1326a36a0303
Autor:
Jianhang Yin, Rusen Lu, Changchang Xin, Yuhong Wang, Xinyu Ling, Dong Li, Weiwei Zhang, Mengzhu Liu, Wutao Xie, Lingyun Kong, Wen Si, Ping Wei, Bingbing Xiao, Hsiang-Ying Lee, Tao Liu, Jiazhi Hu
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Chromosomal structural variations induced by CRISPR/Cas hinder its application in clinics. Here, the authors fuse Cas9 with optimized TREX2 to generate Cas9TX, which can prevent perfect repair and inhibit repeated cleavage.
Externí odkaz:
https://doaj.org/article/25af4229b27742b892b3c9f09814aa2b
Publikováno v:
STAR Protocols, Vol 3, Iss 1, Pp 101088- (2022)
Summary: The repair products of double-stranded DNA breaks (DSBs) are crucial for investigating the mechanism underlying DNA damage repair as well as evaluating the safety and efficiency of gene-editing; however, a comprehensively quantitative assay
Externí odkaz:
https://doaj.org/article/c57c8b5a1ab944baacb0de27e8451b74
Autor:
Zhenhua Luo, Weixu Wang, Feng Li, Zhou Songyang, Xuyang Feng, Changchang Xin, Zhiming Dai, Yuanyan Xiong
Publikováno v:
Molecular Cancer, Vol 18, Iss 1, Pp 1-15 (2019)
Abstract Background Cancer cells become immortalized through telomere maintenance mechanisms, such as telomerase reverse transcriptase (TERT) activation. In addition to maintaining telomere length, TERT activates manifold cell survival signaling path
Externí odkaz:
https://doaj.org/article/cfa42424678e47eaab510cbbc3e92acc
Autor:
Jiazhi Hu, Mengzhu Liu, Chen Ai, Changchang Xin, Jiaxin Li, Weiwei Zhang, Fei-Long Meng, Yafang Shang, Jianhang Yin
Publikováno v:
Nucleic Acids Research
CRISPR–Cas9 generates double-stranded DNA breaks (DSBs) to activate cellular DNA repair pathways for genome editing. The repair of DSBs leads to small insertions or deletions (indels) and other complex byproducts, including large deletions and chro
Autor:
Weiwei Zhang, Jianhang Yin, Jiazhi Hu, Changchang Xin, Zhengrong Zhang-Ding, Chen Ai, Yuhong Wang, Mengzhu Liu
Publikováno v:
Nucleic Acids Research
A series of Cas9 variants have been developed to improve the editing fidelity or targeting range of CRISPR–Cas9. Here, we employ a high-throughput sequencing approach primer-extension-mediated sequencing to analyze the editing efficiency, specifici
Autor:
Jianhang Yin, Rusen Lu, Changchang Xin, Yuhong Wang, Xinyu Ling, Dong Li, Weiwei Zhang, Mengzhu Liu, Wutao Xie, Lingyun Kong, Wen Si, Ping Wei, Bingbing Xiao, Hsiang-Ying Lee, Tao Liu, Jiazhi Hu
Publikováno v:
Nature communications. 13(1)
The mechanism underlying unwanted structural variations induced by CRISPR-Cas9 remains poorly understood, and no effective strategy is available to inhibit the generation of these byproducts. Here we find that the generation of a high level of transl
Autor:
Xuyang Feng, Weixu Wang, Zhiming Dai, Zhenhua Luo, Zhou Songyang, Feng Li, Yuanyan Xiong, Changchang Xin
Publikováno v:
Molecular Cancer
Molecular Cancer, Vol 18, Iss 1, Pp 1-15 (2019)
Molecular Cancer, Vol 18, Iss 1, Pp 1-15 (2019)
Background Cancer cells become immortalized through telomere maintenance mechanisms, such as telomerase reverse transcriptase (TERT) activation. In addition to maintaining telomere length, TERT activates manifold cell survival signaling pathways. How
Autor:
Zhenhua Luo, Weixu Wang, Li, Feng, Songyang, Zhou, Xuyang Feng, Changchang Xin, Zhiming Dai, Yuanyan Xiong
Table S4. Summary of cox regression results. (DOCX 14 kb)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4361d732d0d6f6df2428abdc68771c1c