Isolation and thermal stabilization of mouse ferroportin

Autor: Bo Qiao, So Iwata, Chandrika N. Deshpande, Mika Jormakka, Norimichi Nomura, Elizabeta Nemeth, Josep Font, Bryan Mackenzie, Corbin R. Azucenas, Tomas Ganz, Vicky Xin

Publikováno v: FEBS Open Bio

Here, Deshpande et al. describe the production and purification of mouse ferroportin (Fpn), the only known mammalian iron efflux protein, and selective loop deletion constructs with enhanced thermal stability. Transport activity and hepcidin responsi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c2120c07ef4b9f757eeed046c54cfe4
https://doi.org/10.1002/2211-5463.13039

Integron gene cassettes: a repository of novel protein folds with distinct interaction sites.

Autor: Visaahini Sureshan, Chandrika N Deshpande, Yan Boucher, Jeremy E Koenig, Midwest Center for Structural Genomics, H W Stokes, Stephen J Harrop, Paul M G Curmi, Bridget C Mabbutt

Publikováno v: PLoS ONE, Vol 8, Iss 1, p e52934 (2013)

Mobile gene cassettes captured within integron arrays encompass a vast and diverse pool of genetic novelty. In most cases, functional annotation of gene cassettes directly recovered by cassette-PCR is obscured by their characteristically high sequenc

Externí odkaz: https://doaj.org/article/6e634f80edff4835b698d3756086353e

Calcium is an essential cofactor for metal efflux by the ferroportin transporter family

Autor: Vicky Xin, T Alex Ruwe, Bryan Mackenzie, Kyle R. Vieth, Bo Qiao, Ali Shawki, Mika Jormakka, Chandrika N. Deshpande, Tomas Ganz, Erika V. Valore, Elizabeta Nemeth

Publikováno v: Nature communications, vol 9, iss 1
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
Deshpande, CN; Ruwe, TA; Shawki, A; Xin, V; Vieth, KR; Valore, EV; et al.(2018). Calcium is an essential cofactor for metal efflux by the ferroportin transporter family. NATURE COMMUNICATIONS, 9. doi: 10.1038/s41467-018-05446-4. UCLA: Retrieved from: http://www.escholarship.org/uc/item/0wp7091s
Nature Communications

Ferroportin (Fpn)—the only known cellular iron exporter—transports dietary and recycled iron into the blood plasma, and transfers iron across the placenta. Despite its central role in iron metabolism, our molecular understanding of Fpn-mediated i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::019374d32f398be01e118b12e65275cb
https://escholarship.org/uc/item/0wp7091s

Structure of an atypical FeoB G-domain reveals a putative domain-swapped dimer

Autor: Megan J. Maher, Amy P. Guilfoyle, Josep Font, Mika Jormakka, Aaron P. McGrath, Chandrika N. Deshpande

Publikováno v: Acta Crystallographica Section F Structural Biology and Crystallization Communications. 69:399-404

FeoB is a transmembrane protein involved in ferrous iron uptake in prokaryotic organisms. FeoB comprises a cytoplasmic soluble domain termed NFeoB and a C-terminal polytopic transmembrane domain. Recent structures of NFeoB have revealed two structura

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c7741b6562590e56562cab0cea6b2f6
https://doi.org/10.1107/s1744309113005939

Exploring the correlation between the sequence composition of the nucleotide binding G5 loop of the FeoB GTPase domain (NFeoB) and intrinsic rate of GDP release

Autor: Megan J. Maher, Amy P. Guilfoyle, Mika Jormakka, Chandrika N. Deshpande, Gerhard Schenk

Publikováno v: Bioscience Reports
Bioscience Reports, Vol 34, Iss 6, p e00158 (2014)

GDP release from GTPases is usually extremely slow and is in general assisted by external factors, such as association with guanine exchange factors or membrane-embedded GPCRs (G protein-coupled receptors), which accelerate the release of GDP by seve

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8a9eca1d71b8c392934f4c691f1df1b
https://pubmed.ncbi.nlm.nih.gov/25374115