Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Chandrasekhar, Gurramkonda"'
Autor:
Lynn Wong, Sevda Deldari, Douglas D. Frey, Benjamin Punshon-Smith, Abhay Andar, Antonio Moreira, Mathangi Gopalakrishnan, Joseph Piegols, Shayan Borhani, Xudong Ge, Isabel L. Jackson, Govind Rao, Leah Tolosa, Erick Gutierrez, Rajani Adiga, Chandrasekhar Gurramkonda, Chelsea Wagner, Mike Tolosa, Diana Newman, Andrew Zodda, Yordan Kostov, Yang Liu, David Burgenson, Zeljko Vujaskovic
Publikováno v:
Biotechnology Progress. 36
Protein therapeutics, also known as biologics, are currently manufactured at centralized facilities according to rigorous protocols. The manufacturing process takes months and the delivery of the biological products needs a cold chain. This makes it
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a9acd3dbaa40232e491246fb02e7d0d
https://doi.org/10.1002/btpr.2970
https://doi.org/10.1002/btpr.2970
Autor:
Yordan Kostov, Michael Tolosa, Chandrasekhar Gurramkonda, Tzu-Chiang Han, Joseph E. Taris, David W. Wood, Merideth A. Cooper, Douglas D. Frey, Christina Dinkins, Niloufar Pezeshk, Leah Tolosa, Adil Zuber, Chariz Peñalber-Johnstone, Manohar Pilli, Nicholas Selock, Govind Rao, Kevin Tran
Publikováno v:
Biotechnology and Bioengineering. 115:92-102
The use of cell-free systems to produce recombinant proteins has grown rapidly over the past decade. In particular, cell-free protein synthesis (CFPS) systems based on mammalian cells provide alternative methods for the production of many proteins, i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1077b5b446f6cacebbb0d63098b5bacd
https://doi.org/10.1002/bit.26439
https://doi.org/10.1002/bit.26439
Autor:
Manohar Pilli, Yordan Kostov, Leah Tolosa, Govind Rao, Xudong Ge, Abhay Andar, Chandrasekhar Gurramkonda, David Burgenson
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
Scientific Reports
Scientific Reports
Several groups have recently reported on the utility of cell-free expression systems to make therapeutic proteins, most of them employing CHO or E. coli cell-free extracts. Here, we propose an alternative that uses human blood derived leukocyte cell
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50771a82610820a207ac3170e24b6fe1
Autor:
Erick Gutierrez, David Burgenson, Sevda Deldari, Govind Rao, Chandrasekhar Gurramkonda, Abhay Andar, Douglas D. Frey, Mustafa Al-Adhami, Leah Tolosa, Yordan Kostov
Publikováno v:
Biotechnology and bioengineering. 116(4)
Biopharmaceutical separations require tremendous amounts of optimization to achieve acceptable product purity. Typically, large volumes of reagents and biological materials are needed for testing different parameters, thus adding to the expense of bi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::749716d1738f654a070834b2a9abc4ad
https://pubmed.ncbi.nlm.nih.gov/30450616
https://pubmed.ncbi.nlm.nih.gov/30450616
Autor:
Joshua A. Wilhide, Douglas D. Frey, Adil Zuber, Yordan Kostov, Merideth A. Cooper, Mustafa Al-Adhami, Manohar Pilli, David W. Wood, Antonio Moreira, Chandrasekhar Gurramkonda, Sergei I. Snovida, Sheniqua Brown, Xudong Ge, Yang Liu, David Burgenson, KarunaSri Mupparapu, Abhay Andar, Michael Tolosa, Penny Jensen, Brandon Wagner, Chariz Peñalber-Johnstone, Sudha Veeraraghavan, Krishna Vattem, Benjamin Punshon-Smith, Kanika Taurani, Hui Guo, Sevda Deldari, Aniruddha Rao, Rajani Adiga, Kevin Tran, Leah Tolosa, William R. LaCourse, Dagmawi Tilahun, Priyanka Rauniyar, Govind Rao, Shayan Borhani
Publikováno v:
Nature biomedical engineering. 2(9)
Manufacturing technologies for biologics rely on large, centralized, good-manufacturing-practice (GMP) production facilities and on a cumbersome product-distribution network. Here, we report the development of an automated and portable medicines-on-d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a95fbf6f9c979339db02390d8737bd4
https://pubmed.ncbi.nlm.nih.gov/31015674
https://pubmed.ncbi.nlm.nih.gov/31015674
Publikováno v:
Biotechnology and Applied Biochemistry. 63:57-66
The uses of thermostable starch hydrolytic biocatalysts are steadily increasing for the industrial application because of their obvious need for biocatalytic performance at elevated temperatures. The starch liquefaction and saccharification can be ca
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9d33bb2d24235ecf660e4d50f5064614
https://doi.org/10.1002/bab.1350
https://doi.org/10.1002/bab.1350
Autor:
Yordan Kostov, Shayan Borhani, Sevda Deldari, Niloufar Pezeshk, Aniruddha Rao, David W. Wood, Douglas D. Frey, Krishna Vattem, Leah Tolosa, Manohar Pilli, Xudong Ge, Govind Rao, Michael Tolosa, Tzu-Chiang Han, Chandrasekhar Gurramkonda
Publikováno v:
Biotechnology and bioengineering. 115(5)
Cell-Free Protein Synthesis (CFPS) offers many advantages for the production of recombinant therapeutic proteins using the CHO cell-free system. However, many complex proteins are still difficult to express using this method. To investigate the curre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c982756661d800ab971fda966faec9f5
https://pubmed.ncbi.nlm.nih.gov/29384203
https://pubmed.ncbi.nlm.nih.gov/29384203
Autor:
Mustafa, Al-Adhami, Dagmawi, Tilahun, Govind, Rao, Chandrasekhar, Gurramkonda, Yordan, Kostov
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1571
A portable kinetics fluorometer is developed to detect viable cells which may be contaminating various samples. The portable device acts as a single-excitation, single-emission photometer that continuously measures fluorescence intensity of an indica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::baf956f58432374bb0db8fbf44ce13e8
https://pubmed.ncbi.nlm.nih.gov/28281263
https://pubmed.ncbi.nlm.nih.gov/28281263
Publikováno v:
Biosensors and Biodetection ISBN: 9781493968466
A portable kinetics fluorometer is developed to detect viable cells which may be contaminating various samples. The portable device acts as a single-excitation, single-emission photometer that continuously measures fluorescence intensity of an indica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b84b2215d19f64509b478d54f4ed9fcc
https://doi.org/10.1007/978-1-4939-6848-0_18
https://doi.org/10.1007/978-1-4939-6848-0_18
Publikováno v:
PDA Journal of Pharmaceutical Science and Technology. 68:164-171
A method for rapid detection of microbial detection is presented. It uses the reduction of resazurin to resorufin as an indication of the presence of viable cells. The method is highly sensitive (limit of detection 1 CFU/mL) and rapid (detection time
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::edbf3e8cceafa54e815d3b920cff4fc7
https://doi.org/10.5731/pdajpst.2014.00951
https://doi.org/10.5731/pdajpst.2014.00951