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Autor:
Erin E. Berlew, Keisuke Yamada, Ivan A. Kuznetsov, Eleanor A. Rand, Chandler C. Ochs, Zaynab Jaber, Kevin H. Gardner, Brian Y. Chow
Publikováno v:
ACS Synth Biol
We describe the efficient creation of single-component optogenetic tools for membrane recruitment-based signaling perturbation using BcLOV4 technology. The workflow requires two plasmids to create six different domain arrangements of the dynamic memb