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Machine learning-coupled combinatorial mutagenesis enables resource-efficient engineering of CRISPR-Cas9 genome editor activities.

Autor: Thean DGL; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, SAR, China., Chu HY; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, SAR, China.; Centre for Oncology and Immunology Limited, Hong Kong Science Park, Hong Kong, SAR, China., Fong JHC; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, SAR, China., Chan BKC; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, SAR, China.; Centre for Oncology and Immunology Limited, Hong Kong Science Park, Hong Kong, SAR, China., Zhou P; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, SAR, China.; Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong, SAR, China., Kwok CCS; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, SAR, China., Chan YM; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, SAR, China., Mak SYL; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, SAR, China., Choi GCG; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, SAR, China.; Centre for Oncology and Immunology Limited, Hong Kong Science Park, Hong Kong, SAR, China., Ho JWK; School of Biomedical Sciences, The University of Hong Kong, Hong Kong, SAR, China.; Laboratory of Data Discovery for Health Limited (D24H), Hong Kong Science Park, Hong Kong, SAR, China., Zheng Z; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, SAR, China.; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, SAR, China.; Biotechnology and Health Centre, City University of Hong Kong Shenzhen Research Institute, Shenzhen, China., Wong ASL; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, SAR, China. aslw@hku.hk.; Centre for Oncology and Immunology Limited, Hong Kong Science Park, Hong Kong, SAR, China. aslw@hku.hk.; Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong, SAR, China. aslw@hku.hk.

Publikováno v: Nature communications [Nat Commun] 2022 Apr 25; Vol. 13 (1), pp. 2219. Date of Electronic Publication: 2022 Apr 25.

A Combinatorial CRISPR-Cas9 Screen Identifies Ifenprodil as an Adjunct to Sorafenib for Liver Cancer Treatment.

Autor: Xu F; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Tong M; School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; State Key Laboratory of Liver Research, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Tong CSW; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Chan BKC; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Chu HY; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Wong TL; School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Fong JHC; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Cheung MSH; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Mak KH; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Pardeshi L; Faculty of Health Sciences, University of Macau, Macau SAR, China.; Genomics and Bioinformatics Core, Faculty of Health Sciences, University of Macau, Macau SAR, China., Huang Y; School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; Department of Statistics and Actuarial Science, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Wong KH; Faculty of Health Sciences, University of Macau, Macau SAR, China.; Institute of Translational Medicine, University of Macau, Avenida da Universidade, Taipa, Macau SAR, China.; MoE Frontiers Science Center for Precision Oncology, University of Macau, Macau SAR, China., Choi GCG; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Ma S; School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. aslw@hku.hk stefma@hku.hk.; The University of Hong Kong-Shenzhen Hospital, Shenzhen, China., Wong ASL; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. aslw@hku.hk stefma@hku.hk.; Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.

Publikováno v: Cancer research [Cancer Res] 2021 Dec 15; Vol. 81 (24), pp. 6219-6232. Date of Electronic Publication: 2021 Oct 19.

A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets.

Autor: Zhou P; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Chan BKC; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Wan YK; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Yuen CTL; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Choi GCG; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Li X; School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Tong CSW; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Zhong SSW; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Sun J; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Bao Y; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong SAR, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong SAR, China., Mak SYL; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong SAR, China., Chow MZY; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong SAR, China., Khaw JV; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Leung SY; Department of Pathology, The University of Hong Kong, Pokfulam, Hong Kong SAR, China; Centre for PanorOmic Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China; The Jockey Club Centre for Clinical Innovation and Discovery, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Zheng Z; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong SAR, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong SAR, China; Biotechnology and Health Centre, City University of Hong Kong Shenzhen Research Institute, Shenzhen, China., Cheung LWT; School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Tan K; Faculty of Health Sciences, University of Macau, Macau SAR, China; Genomics, Bioinformatics and Single Cell Analysis Core, Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau SAR, China., Wong KH; Faculty of Health Sciences, University of Macau, Macau SAR, China; Institute of Translational Medicine, University of Macau, Avenida da Universidade, Taipa, Macau SAR, China., Chan HYE; Laboratory of Drosophila Research, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China; Gerald Choa Neuroscience Centre, The Chinese University of Hong Kong, Hong Kong SAR, China., Wong ASL; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China; Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. Electronic address: aslw@hku.hk.

Publikováno v: Cell reports [Cell Rep] 2020 Aug 11; Vol. 32 (6), pp. 108020.

Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9.

Autor: Choi GCG; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China., Zhou P; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China., Yuen CTL; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China., Chan BKC; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China., Xu F; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China., Bao S; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, China., Chu HY; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, China., Thean D; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China., Tan K; Faculty of Health Sciences, University of Macau, Macau, China.; Genomics, Bioinformatics and Single Cell Analysis Core, Faculty of Health Sciences, University of Macau, Macau, China., Wong KH; Faculty of Health Sciences, University of Macau, Macau, China.; Institute of Translational Medicine, University of Macau, Macau, China., Zheng Z; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, China.; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.; Biotechnology and Health Centre, City University of Hong Kong Shenzhen Research Institute, Shenzhen, China., Wong ASL; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China. aslw@hku.hk.; Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong, China. aslw@hku.hk.

Publikováno v: Nature methods [Nat Methods] 2019 Aug; Vol. 16 (8), pp. 722-730. Date of Electronic Publication: 2019 Jul 15.