Výsledky vyhledávání - "Chamitha J. Weeramange"

The strengths and limitations of using biolayer interferometry to monitor equilibrium titrations of biomolecules

Autor: Dipika Singh, Chamitha J. Weeramange, Liskin Swint-Kruse, Max S. Fairlamb, Aron W. Fenton

Publikováno v: Protein Sci

Every method used to quantify biomolecular interactions has its own strengths and limitations. To quantify protein‐DNA binding affinities, nitrocellulose filter binding assays with (32)P‐labeled DNA quantify K (d) values from 10(−12) to 10(−8

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ceedc2b1738bd0a651e4f64726cbb414
https://doi.org/10.1002/pro.3827

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Homolog comparisons further reconcile in vitro and in vivo correlations of protein activities by revealing over‐looked physiological factors

Autor: Chamitha J. Weeramange, Liskin Swint-Kruse, Kristen M. Schwingen, Joshua J. Riepe, Sudheer Tungtur

Publikováno v: Protein Sci

To bridge biological and biochemical disciplines, the relationship between in vitro protein biochemical function and in vivo activity must be established. Such studies can (a) help determine whether properties measured in simple, dilute solutions ext

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b57f01d835f4eae5ec379fc09e6ccc44
https://doi.org/10.1002/pro.3695

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Reconcilingin vitroandin vivoactivities of engineered, LacI-based repressor proteins: Contributions of DNA looping and operator sequence variation

Autor: Sudheer Tungtur, Chamitha J. Weeramange, Liskin Swint-Kruse, Kristen M. Schwingen, Joshua J. Riepe

One way to create new components for synthetic transcription circuits is to re-purpose naturally occurring transcription factor proteins and their cognate DNA operators. For the proteins, re-engineering can be accomplished via domain recombination (t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38c17ff1776ac93860e21dc2470c2fc7
https://doi.org/10.1101/477893

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Protein-protein interactions with fructose-1-kinase alter function of the central Escherichia coli transcription regulator, Cra

Autor: Chamitha J. Weeramange, Rau Bf, Kelly S. Harrison, Singh D, Max S. Fairlamb, Sudheer Tungtur, Hefty Ps, Liskin Swint-Kruse, Sarah Meinhardt, Aron W. Fenton

SummaryInE. coli, the master transcription regulator Cra regulates >100 genes in central metabolism by binding upstream DNA operator sequences. Genes encoding glycolytic enzymes are repressed, whereas those for gluconeogenesis and the citric acid cyc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1dc20d4725546fd67c3f08c10c141604

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