Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Chad O'Gorman"'
Publikováno v:
Biology Open, Vol 7, Iss 10 (2018)
Ubiquitin A-52 residue ribosomal protein fusion product 1 (Uba52), a ubiquitin-ribosomal fusion gene, is a major source of ubiquitin protein for covalent modification of proteinaceous substrates recycled by ubiquitin-proteasome system (UPS). Its role
Externí odkaz:
https://doaj.org/article/1f3d19c8d3e742aaaa5d1b7fb43fd47b
Autor:
Randall S. Prather, Stephanie L. Murphy, Bethany K. Redel, Chad O'Gorman, Benjamin P. Beaton, Lee D. Spate, Anna Spate, Joshua A. Benne, Kevin D. Wells
Publikováno v:
BioTechniques. 64:118-124
Production of Cas9 mRNA in vitro typically requires the addition of a 5´ cap and 3´ polyadenylation. A plasmid was constructed that harbored the T7 promoter followed by the EMCV IRES and a Cas9 coding region. We hypothesized that the use of the met
Autor:
Chad O'Gorman, Kiho Lee, Ming-Tao Zhao, Kristin M. Whitworth, Randall S. Prather, Kevin D. Wells, Lee D. Spate, Melissa Samuel, Eric M. Walters, Jiude Mao, Clifton N. Murphy, Rocío Melissa Rivera
Publikováno v:
Cellular Reprogramming. 17:28-40
Faulty epigenetic reprogramming of somatic nuclei is thought to be the main reason for low cloning efficiency by somatic cell nuclear transfer (SCNT). Histone deacetylase inhibitors (HDACi), such as Scriptaid, improve developmental competence of SCNT
Autor:
Kevin D. Wells, Anna Spate, A. K. Byrne, Kristin M. Whitworth, Chad O'Gorman, Randall S. Prather
Publikováno v:
Reproduction, Fertility and Development. 29:120
The use of the CRISPR/Cas9 system has become increasingly popular for creating gene edits in both cell and embryo culture. High specificity and efficiency of editing as well as low cost and ease of use has helped to promote its use. We hypothesised t
Autor:
Kristin M. Whitworth, Stephanie L. Murphy, Kiho Lee, Clifton N. Murphy, Kevin D. Wells, Benjamin P. Beaton, Alan J. Mileham, Randall S. Prather, Eric M. Walters, Lee D. Spate, John P. Driver, Melissa Samuel, Joshua A. Benne, Chad O'Gorman, Jiude Mao, David G. McLaren
Publikováno v:
Biology of Reproduction. 91
Targeted modification of the pig genome can be challenging. Recent applications of the CRISPR/Cas9 system hold promise for improving the efficacy of genome editing. When a designed CRISPR/Cas9 system targeting CD163 or CD1D was introduced into somati
Autor:
Jianguo Zhao, Chad O'Gorman, Young-Joo Yi, Kevin D. Wells, Peter Sutovsky, Eric M. Walters, Miriam Sutovsky, Randall S. Prather, Edward L. Miles, Melissa Samuel
Among its many functions, the ubiquitin–proteasome system regulates substrate-specific proteolysis during the cell cycle, apoptosis, and fertilization and in pathologies such as Alzheimer’s disease, cancer, and liver cirrhosis. Proteasomes are pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3fc1833ea46057d89efaaae7b59b1ad9
https://europepmc.org/articles/PMC3631692/
https://europepmc.org/articles/PMC3631692/
Publikováno v:
Reproduction, Fertility and Development. 23:138
Beckwith-Wiedeman Syndrome (BWS) is a loss of imprinting (LOI) condition that is associated with macroglossia, midline abdominal defects, and neonatal gigantism among other symptoms. These symptoms have also been seen in animals produced by SCNT. A c
Autor:
Kevin D. Wells, Melissa Samuel, Jianguo Zhao, Eric M. Walters, Peter Sutovsky, Miriam Sutovsky, Randall S. Prather, Chad O'Gorman
Publikováno v:
Reproduction, Fertility and Development. 23:263
Proteasomes are large protein complexes involved in protein degradation in eukaryotes and undergo dynamic redistribution between cellular compartments. Characterising the cellular localization of proteasomes at various stages of development and in re