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pro vyhledávání: '"Chad J. Borchert"'
Autor:
Calvin F. Roff, David R. Cahill, Keith G. Godfrey, Mark J. Schendel, Amy M. Walz, Paul A. Saunders, Igor J. Woyno, Chad J. Borchert, Ryan T. Loegering, Frank Mortari, Alexander E. Kaluyzhny, Jeffrey A. Cooper, Michelle M. Roodell, Harvey Gaylord, Dorothy A. Schroeder, Richard A. Krzyzek, Monica Tsang, Amy L. Isaacson
Publikováno v:
Analytical Biochemistry. 284:114-124
We describe an enzyme-linked immunosorbent assay (ELISA) for quantifying relative amounts of active caspase 3 in apoptotic cells. Covalent modification of caspase 3 active sites with a biotinylated inhibitor differentiates active from latent caspases