Zobrazeno 1 - 10
of 73
pro vyhledávání: '"Cellvibrio gilvus"'
Publikováno v:
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology
Abstract β-Glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of β-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of β-glucans as major bioactive compounds.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::789ab9d07197c9e6ff4778d183e4e24f
https://doi.org/10.1007/s00253-021-11320-z
https://doi.org/10.1007/s00253-021-11320-z
Autor:
Truong Thi Hoa, Ajay Singh, Kenji Iwakiri, Chika Aoyagi, Ken Tokuyasu, Kunio Ohmiya, Kiyoshi Hayashi
Publikováno v:
Brazilian Archives of Biology and Technology, Vol 42, Iss 3, Pp 303-306 (1999)
Based on homology search in the amino acid sequences, a chimeric ß-glucosidase with enzyme activity has been prepared by replacing C-terminal region of Thr509-Arg646 from Cellvibrio gilvus with N-terminal region of Arg160-Gly283 from Ruminococcus al
Externí odkaz:
https://doaj.org/article/c3c723825c2b44d590d54476b9c4dd1c
Autor:
Tom Desmet, Giang Hai Tran, M.R.M. De Groeve, Wim Soetaert, Jan Stout, Savvas N. Savvides, A. Van Hoorebeke
Publikováno v:
ANALYTICAL BIOCHEMISTRY
Glycoside phosphorylases (GPs) are interesting enzymes for the glycosylation of chemical molecules. They require only a glycosyl phosphate as sugar donor and an acceptor molecule with a free hydroxyl group. Their narrow substrate specificity, however
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b307bcbd5941add8b7bc5bf399c7259
https://doi.org/10.1016/j.ab.2010.02.028
https://doi.org/10.1016/j.ab.2010.02.028
Autor:
Martin J. Baumann, Jens Ø. Duus, Yvonne Westphal, Bent O. Petersen, Hiroyuki Nakai, Maher Abou Hachem, Karin Mannerstedt, Adiphol Dilokpimol, Henk A. Schols, Birte Svensson
Publikováno v:
Nakai, H, Hachem, M A, Petersen, B O, Westphal, Y, Mannerstedt, K, Baumann, M J, Dilokpimol, A, Schols, H A, Duus, J Ø & Svensson, B 2010, ' Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from Clostridium thermocellum ', Biochimie, vol. 92, no. 12, pp. 1818-26 . https://doi.org/10.1016/j.biochi.2010.07.013
Biochimie 92 (2010) 12
Biochimie, 92(12), 1818-1826
Biochimie 92 (2010) 12
Biochimie, 92(12), 1818-1826
Inverting cellobiose phosphorylase (CtCBP) and cellodextrin phosphorylase (CtCDP) from Clostridium thermocellum ATCC27405 of glycoside hydrolase family 94 catalysed reverse phosphorolysis to produce cellobiose and cellodextrins in 57% and 48% yield f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::217238c37d3dc9786f51b6ca6d1ceed3
https://doi.org/10.1016/j.biochi.2010.07.013
https://doi.org/10.1016/j.biochi.2010.07.013
Publikováno v:
Biotechnology and Bioprocess Engineering. 14:266-273
In order to create novel β-glucosidase constructs, 8 kinds of chimeric β-glucosidases were constructed using overlapping polymerase chain reaction (PCR) based on Cellvibrio gilvus (CG) and Thermotoga maritima (TM) genes. Two kinds of novel chimeric
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::334b57975d9557702e67bd082c7af82c
https://doi.org/10.1007/s12257-008-0255-5
https://doi.org/10.1007/s12257-008-0255-5
Publikováno v:
Carbohydrate Research. 305:543-548
β- d - Glc p-(1 → 4)- d - Ara p, β- d - Glc p-(1 → 4)- l - Fuc p , and β- d - Glc p-(1 → 4)- d - Alt p were synthesised using a partially purified cellobiose phophorylase from Cellvibrio gilvus . The disaccharides were purified, and analysed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a173009f42ce78e9ce19d815faf7ec61
https://doi.org/10.1016/s0008-6215(97)00247-4
https://doi.org/10.1016/s0008-6215(97)00247-4
Publikováno v:
Carbohydrate Research. 275:67-72
The disaccharides 4-O-β- d -glucopyranosyl- d -glucosamine (Glc-GlcN) and 4-O-β- d -glucopyranosyl-2-deoxy- d -glucose (Glc-2-deoxy-Glc) were synthesized from equimolar amounts of d -glucosamine and α- d -glucose-1-phosphate (G-1-P), and 2-deoxy-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d1b04c06c11c7c247531e37ef20a6863
https://doi.org/10.1016/0008-6215(95)00143-h
https://doi.org/10.1016/0008-6215(95)00143-h
Publikováno v:
Biochemical Journal. 305:715-719
The amino acid sequences of beta-glucosidases from Cellvibrio gilvus and Agrobacterium tumefaciens show significant similarity in most of the parts. However, the pH/temperature optima and stabilities of the two enzymes are quite different. C. gilvus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64ed7993ec39c8011d40c0ae38fc1033
https://doi.org/10.1042/bj3050715
https://doi.org/10.1042/bj3050715
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 56:652-655
Cellobiose phosphorylase was purified from Cellvibrio gilvus cells by the method reported previously with some modifications, and its kinetic properties were studied in detail. The initial velocity of the synthetic reaction was 1.4 times as fast as t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b6c97e9ce78a69da83282119e11c916
https://doi.org/10.1271/bbb.56.652
https://doi.org/10.1271/bbb.56.652
Publikováno v:
Agricultural and Biological Chemistry. 55:1959-1967
An enzyme component that hydrolyzes pNP-G2 but not CMC has been isolated from a culture broth of Cellvibrio gilvus by a multi-step procedure involving Butyl-Toyopearl, DEAE-Toyopearl, and CM-Toyopearl chromatographies. The purified enzyme gave a sing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6801c0915e293e2701ccc680b6acb6f
https://doi.org/10.1271/bbb1961.55.1959
https://doi.org/10.1271/bbb1961.55.1959