Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Cell-Free System/*metabolism"'
Autor:
Jorge E. Azevedo, Ana F. Dias, Tânia Francisco, Cláudia P. Grou, Tony A. Rodrigues, Ana G. Pedrosa
Publikováno v:
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Here we describe a protocol to dissect the peroxisomal matrix protein import pathway using a cell-free in vitro system. The system relies on a postnuclear supernatant (PNS), which is prepared from rat/mouse liver, to act as a source of peroxisomes an
Autor:
Nasioulas G, Papadopoulou E, Koliopanos A, Dardoufas K, Davilas E, Sotiriou, Aggelakis F, Agnanti Nj, Karydas I
Publikováno v:
Oncology research. 14(9)
Extracellular nucleic acids could serve as molecular markers in the early detection of cancer and in the prediction of disease outcome. In this study we examined six molecular markers, such as: variations in the quantity of DNA in plasma, glutathione
Autor:
Laure Moutouh-de Parseval, Jacques Corbeil, Pascal Schneider, Damien Arnoult, Jean-Claude Ameisen, Jean-Daniel Lelièvre, Allan J. Hance, Jérôme Estaquier, Frédéric Petit
Publikováno v:
Journal of Biological Chemistry, vol. 277, no. 2, pp. 1477-1487
We have explored in vitro the mechanism by which human immunodeficiency virus, type 1 (HIV-1) induces cell death of primary CD4+ T cells in conditions of productive infection. Although HIV-1 infection primed phytohemagglutinin-activated CD4+ T cells
Autor:
V. Nathan Subramaniam, Line Roy, Ali Fazel, D. James Morré, Wanjin Hong, Christine Lavoie, Rob Hendriks, John J.M. Bergeron, Amélie Pelletier, Jennifer N. Gushue, Jacques Paiement
Publikováno v:
Molecular Biology of the Cell
Transitional endoplasmic reticulum (tER) consists of confluent rough and smooth endoplasmic reticulum (ER) domains. In a cell-free incubation system, low-density microsomes (1.17 g cc− 1) isolated from rat liver homogenates reconstitute tER by Mg2+
Autor:
Geneviève Porcheron, Anne Gilbert, Jean-Pierre Paccaud, Jacqueline Balz, Michelangelo Foti, Jean-Louis Carpentier
Publikováno v:
Scopus-Elsevier
Journal of Cell Science, Vol. 112 ( Pt 7) (1999) pp. 1101-1110
Journal of Cell Science, Vol. 112 ( Pt 7) (1999) pp. 1101-1110
The endocytic function of caveolae was challenged by taking advantage of a cell-free assay directly measuring the detachment of receptor-containing vesicles from isolated plasma membranes. Plasma membranes from cultured cells surface-labeled with 125