Zobrazeno 1 - 10
of 2 543
pro vyhledávání: '"Cell lysis"'
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024)
Abstract Metagenomic analysis has enabled insights into soil community structure and dynamics. Long-read sequencing for metagenomics can enhance microbial ecology by improving taxonomic classification, genome assembly, and functional annotation. Howe
Externí odkaz:
https://doaj.org/article/72249bb7a3be42a0aa215f9444af52bc
Publikováno v:
Biotechnology for Biofuels and Bioproducts, Vol 17, Iss 1, Pp 1-12 (2024)
Abstract Background Lipids produced using oleaginous yeast cells are an emerging feedstock to manufacture commercially valuable oleochemicals ranging from pharmaceuticals to lipid-derived biofuels. Production of biofuels using oleaginous yeast is a m
Externí odkaz:
https://doaj.org/article/908f40693ac3477c8268526f638786aa
Autor:
Jason D. Limberis, John Z. Metcalfe
Publikováno v:
HardwareX, Vol 19, Iss , Pp e00576- (2024)
turboLysis is a novel mechanical cell lysis device that utilizes small beads to efficiently lyse tough cells like Mycobacterium, Saccharomyces, and Arabidopsis. We compared turboLysis to bead beating using the BeadBug 6 for several concentrations of
Externí odkaz:
https://doaj.org/article/8eb386147ac746f78073ea43b29db2e0
Autor:
Supathep Tansirichaiya, Kittikun Songsomboon, Nichamon Chaianant, Wasawat Lertsivawinyu, Mohammed Al‐Haroni
Publikováno v:
Clinical and Experimental Dental Research, Vol 10, Iss 4, Pp n/a-n/a (2024)
Abstract Objectives The human oral microbiome, a complex ecosystem linked to oral and systemic health, harbors a diverse array of microbial populations, including antimicrobial resistance genes (ARGs). As a critical component of the One Health approa
Externí odkaz:
https://doaj.org/article/fc082f8b20f943fe965736afb68e0f4d
Publikováno v:
Current Issues in Molecular Biology, Vol 46, Iss 1, Pp 621-633 (2024)
In this study, we review the properties of three anionic detergents, sodium dodecyl sulfate (SDS), Sarkosyl, and sodium lauroylglutamate (SLG), as they play a critical role in molecular biology research. SDS is widely used in electrophoresis and cell
Externí odkaz:
https://doaj.org/article/9af6536fee8e40068c6a646e14128476
Autor:
Chia-Wei Liu, Hideaki Tsutsui
Publikováno v:
SLAS Technology, Vol 28, Iss 5, Pp 302-323 (2023)
Efficient sample preparation and accurate disease diagnosis under field conditions are of great importance for the early intervention of diseases in humans, animals, and plants. However, in-field preparation of high-quality nucleic acids from various
Externí odkaz:
https://doaj.org/article/2d3924c1ccc8438b8859ce79c5ddf125
Autor:
Karolien Buyl, Makram Merimi, Robim M. Rodrigues, Saida Rahmani, Mohammad Fayyad-Kazan, Fatima Bouhtit, Noureddine Boukhatem, Tamara Vanhaecke, Hassan Fahmi, Joery De Kock, Mehdi Najar
Publikováno v:
Biomolecules, Vol 14, Iss 7, p 852 (2024)
Background: AT-MSCs display great immunoregulatory features, making them potential candidates for cell-based therapy. This study aimed to evaluate the “RBC lysis buffer” isolation protocol and immunological profiling of the so-obtained AT-MSCs. M
Externí odkaz:
https://doaj.org/article/ef1210ad818c4be8bbd3a1ff36e37c92
Publikováno v:
Engineering in Life Sciences, Vol 24, Iss 1, Pp n/a-n/a (2024)
Abstract We developed a microchip device using surface acoustic waves (SAW) and sharp‐edge glass microparticles to rapidly lyse low‐level cell samples. This microchip features a 13‐finger pair interdigital transducer (IDT) with a 30‐degree fo
Externí odkaz:
https://doaj.org/article/efbbbbadbc16434bbc0ad902c43d7e6e
Autor:
Elham R. S. SOLIMAN
Publikováno v:
Acta Agriculturae Slovenica, Vol 119, Iss 4 (2023)
Ricinus communis seeds harbor high oil and polyphenolics contents that hinder DNA extraction. Here, a rapid and efficient protocol for isolating total DNA from R. communis seeds was developed. The current method implies the use of repeated cycles of
Externí odkaz:
https://doaj.org/article/1b60ec5d32ec4b55a734e5db80bef7ac
Publikováno v:
Microbial Cell Factories, Vol 22, Iss 1, Pp 1-16 (2023)
Abstract Background Intracellular biomacromolecules, such as industrial enzymes and biopolymers, represent an important class of bio-derived products obtained from bacterial hosts. A common key step in the downstream separation of these biomolecules
Externí odkaz:
https://doaj.org/article/c1d9511efd0249d19ec663819ffbd71a