Zobrazeno 1 - 10
of 4 557
pro vyhledávání: '"Cell isolation"'
Publikováno v:
Journal of Materials Research and Technology, Vol 33, Iss , Pp 874-883 (2024)
Microfluidic technology utilizing the deterministic lateral displacement (DLD) method holds significant promise for efficiently separating micro-particles and biological cells. Despite the notable high throughput advantages associated with DLD chips,
Externí odkaz:
https://doaj.org/article/a6550e4d7af0419aa3e7d77df35a9bd3
Publikováno v:
Plant Methods, Vol 20, Iss 1, Pp 1-11 (2024)
Abstract Background With the rapid development of single-cell sequencing technology, histological studies are no longer limited to conventional homogenized tissues. Laser microdissection enables the accurate isolation of specific tissues or cells, an
Externí odkaz:
https://doaj.org/article/1d19dcd318a84ae29ef7f1ee055c547b
Publikováno v:
Journal of Biological Engineering, Vol 18, Iss 1, Pp 1-18 (2024)
Abstract Background There is significant interest in isolating cells of the blood-brain barrier (BBB) for use in in vitro screening of therapeutics and analyzing cell specific roles in neurovascular pathology. Primary brain cells play an advantageous
Externí odkaz:
https://doaj.org/article/0ad954a91ecf48e9b4f7e967dd786507
Autor:
Yaling Huang, Yisha Wu, Shikai Han, Qiaoling Wang, Guomingxiu Cong, Zhongzhen Liu, Shuyan Guan, Xiaojuan Huang, Ying Liu, Jianhua Yin, Jinmei Xue, Chuanyu Liu
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103298- (2024)
Summary: Examining nasal mucosa samples is crucial for nasal cavity disease research and diagnosis. Simultaneously obtaining high-quality data for single-cell transcriptomics (single-cell RNA sequencing [scRNA-seq]) and epigenomics (single-cell assay
Externí odkaz:
https://doaj.org/article/f04802d27fb74c6caf81f746d18e65e1
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103288- (2024)
Summary: In human sepsis, myelocytosis and concomitant lymphopenia complicate the study of peripheral blood natural killer (NK) cells. Here, we present a protocol for isolating NK cells from peripheral blood of septic patients using magnetic cell sep
Externí odkaz:
https://doaj.org/article/97ada2afd25b49608b18203dd98e4e94
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103267- (2024)
Summary: Bacillus Calmette-Guérin (BCG), the only licensed tuberculosis vaccine, provides non-specific protection against non-tuberculosis diseases that is mediated by trained immunity, a functional reprogramming mediated by innate immune memory. He
Externí odkaz:
https://doaj.org/article/8700c60ebeb54c249d0da943d433eefe
Autor:
Satoshi Matsui, Joseph R. Shiley, Morgan Buckley, Hee-Woong Lim, Yueh-Chiang Hu, Christopher N. Mayhew, Makiko Iwafuchi
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103233- (2024)
Summary: Transcription factor (TF) gene knockout or knockdown experiments provide comprehensive downstream effects on gene regulation. However, distinguishing primary direct effects from secondary effects remains challenging. To assess the direct eff
Externí odkaz:
https://doaj.org/article/652ed679bdfb414bbbb062e5a1bc2a1d
Autor:
Shefaa AlAsfoor, Cheryl E. Bernard, Erik Jessen, Vernadette A. Simon, Fariborz Rakhshan, Gianluca Cipriani, Linda C. Hsi, Jack A. Patraw, Kari J. Carothers, Julie S. Lau, Samantha J. McDonough, Surendra Dasari, Gianrico Farrugia, Madhusudan Grover
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103258- (2024)
Summary: Understanding the diversity of gastrointestinal (GI) immune cells, especially in the muscularis propria, is crucial for understanding their role in the maintenance of enteric neurons and smooth muscle and their contribution to GI motility. H
Externí odkaz:
https://doaj.org/article/c451e2ad6c914ccba7a3b09314efade5
Autor:
Anna Carey, Christina D. Camell
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103227- (2024)
Summary: Adipose tissue immune cells are heterogeneous and dynamic, alter metabolism, and drive immune responses. Here, we present a protocol for assessment and characterization of murine adipose tissue immune cells using fluorescence-based flow cyto
Externí odkaz:
https://doaj.org/article/3d3709c6b93e4c358b8fd17c5c7ac3f7
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103183- (2024)
Summary: Extracellular vesicles (EVs) are membranous nanoparticles classified based on their size and surface markers, which can be specific to various cell origins. Here, we present a protocol for the isolation of pulmonary-specific EVs in mice. We
Externí odkaz:
https://doaj.org/article/5aaba410c20d4d9f8c42aa87c565ae98