Zobrazeno 1 - 10
of 2 570
pro vyhledávání: '"Cell based assays"'
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103292- (2024)
Summary: RNA-binding proteins (RBPs) are involved in many biological processes. The direct interaction between protein and RNA can be studied using cross-linking immunoprecipitation (CLIP) techniques in living cells. Here, we present a protocol to ch
Externí odkaz:
https://doaj.org/article/1412586122da49d99bdad530739e8e21
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103284- (2024)
Summary: Quantifying the number of proteins that interact with mRNAs, in particular with poly(A) tails of mRNAs, is crucial for understanding gene regulation. Biochemical assays offer significant advantages for this purpose. Here, we present a protoc
Externí odkaz:
https://doaj.org/article/e38281f97c174f3a8a092cadd9549a2c
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103181- (2024)
Summary: Here, we present a protocol to comprehensively quantify autophagy initiation using the readout of the microtubule associated protein 1 light chain 3 beta (LC3B) Förster’s resonance energy transfer (FRET) biosensor. We describe steps for c
Externí odkaz:
https://doaj.org/article/b906862a8c774995b954e0c3dd7db5f1
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103243- (2024)
Summary: In vivo models of brain pathology are crucial for studying neurological diseases. Here, we present a protocol to induce a pathological condition in a mouse brain area by local injection of neurotoxic stimulus. We describe steps for preparing
Externí odkaz:
https://doaj.org/article/e07310d47f584db0a5190d4c05adc87a
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103208- (2024)
Summary: Protein kinase C-δ (PKC-δ) is a key enzyme controlling growth, differentiation, and apoptosis in various cells, including immune cells. Here, we present a protocol to determine PKC-δ activation in response to increased membrane-bound diac
Externí odkaz:
https://doaj.org/article/b627674bb4454d0da0c2ed8adaab8935
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103215- (2024)
Summary: Efficient macrophage efferocytosis maintains homeostasis and resolves inflammation. Here, we provide a protocol to assess the engulfment and acidification of apoptotic cells (ACs) by macrophages. We describe steps for preparing bone marrow-d
Externí odkaz:
https://doaj.org/article/bf4689e0d14a4b099297a686518b504f
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103228- (2024)
Summary: Bioluminescence resonance energy transfer (BRET) is widely employed for real-time monitoring of G protein-coupled receptor activity, interactions, and trafficking in heterologous cell lines, yet its use in neuronal systems remains limited. H
Externí odkaz:
https://doaj.org/article/77cff974e6fa48a489ef8a8d51dd07b0
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103202- (2024)
Summary: Leukemia niche impacts quiescence; however, culturing patient-derived samples ex vivo is technically challenging. Here, we present a protocol for in vitro co-culture of patient-derived xenograft acute lymphoblastic leukemia (PDX-ALL) cells w
Externí odkaz:
https://doaj.org/article/75fd9bad6bd74920afe3d28544b300b6
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103204- (2024)
Summary: Here, we present a protocol for the quantitative assessment of rat and mouse cardiomyocyte proliferation both in vitro and in vivo. For the in vivo approach, we describe steps for the isolation of neonatal rat cardiomyocytes and the employme
Externí odkaz:
https://doaj.org/article/bcd8e4d40dfb484d99c7f53b4fd20748
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103174- (2024)
Summary: Isolating high-quality different cell types is a powerful approach for understanding cellular compositions and features in the heart, but it is challenging. The available protocols typically focus on isolating one or two cell types. Here, we
Externí odkaz:
https://doaj.org/article/517d45c4278447b3919605137706581e