Zobrazeno 1 - 10
of 84
pro vyhledávání: '"Cell Culture Techniques/methods"'
Autor:
Nicholas A. Kurniawan, Carlijn V. C. Bouten, Thomas E. Woud, Maaike Bril, Mirko D’Urso, Cas van der Putten
Publikováno v:
Journal of Visualized Experiments (JoVE), 184:e63988. MYJoVE Corporation
Journal of Visualized Experiments
Journal of Visualized Experiments
The extracellular matrix is an important regulator of cell function. Environmental cues existing in the cellular microenvironment, such as ligand distribution and tissue geometry, have been increasingly shown to play critical roles in governing cell
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2d8722480bd948d4194481974e11abc
https://doi.org/10.3791/63988
https://doi.org/10.3791/63988
Autor:
Nicholas R. Casewell, Mátyás A. Bittenbinder, Hans Clevers, Harald M. I. Kerkkamp, Jens Puschhof, Yorick Post, Freek J. Vonk, Joep Beumer, Michael K. Richardson
Publikováno v:
Nature Protocols, 16(3), 1494-1510. Nature Publishing Group
Puschhof, J, Post, Y, Beumer, J, Kerkkamp, H M, Bittenbinder, M, Vonk, F J, Casewell, N R, Richardson, M K & Clevers, H 2021, ' Derivation of snake venom gland organoids for in vitro venom production ', Nature Protocols, vol. 16, no. 3, pp. 1494-1510 . https://doi.org/10.1038/s41596-020-00463-4
Puschhof, J, Post, Y, Beumer, J, Kerkkamp, H M, Bittenbinder, M, Vonk, F J, Casewell, N R, Richardson, M K & Clevers, H 2021, ' Derivation of snake venom gland organoids for in vitro venom production ', Nature Protocols, vol. 16, no. 3, pp. 1494-1510 . https://doi.org/10.1038/s41596-020-00463-4
More than 400,000 people each year suffer adverse effects following bites from venomous snakes. However, snake venom is also a rich source of bioactive molecules with known or potential therapeutic applications. Manually ‘milking’ snakes is the m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abd30f8baead05384455572a94b2aa20
http://www.scopus.com/inward/record.url?scp=85099969454&partnerID=8YFLogxK
http://www.scopus.com/inward/record.url?scp=85099969454&partnerID=8YFLogxK
Publikováno v:
Nature Protocols, 15(10), 3380-3409. Nature Publishing Group
Adult stem cell-based organoid technology is a versatile tool for the generation and long-term maintenance of near-native 3D epithelial tissues in vitro. The generation of cancer organoids from primary patient material enables a range of therapeutic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4da1ff3d68d0b2dc260656e8f476421f
https://doi.org/10.1038/s41596-020-0379-4
https://doi.org/10.1038/s41596-020-0379-4
Autor:
Suvi T. Häkkinen, Raija-Liisa Heiniö, Heli Nygren, Natalia Maiorova, Anneli Ritala, Riitta Puupponen-Pimiä, Liisa Nohynek, Heiko Rischer
Publikováno v:
Plant Cell Reports
Häkkinen, S T, Nygren, H, Nohynek, L, Puupponen-Pimiä, R, Heiniö, R L, Maiorova, N, Rischer, H & Ritala, A 2020, ' Plant cell cultures as food—aspects of sustainability and safety ', Plant Cell Reports, vol. 39, no. 12, pp. 1655–1668 . https://doi.org/10.1007/s00299-020-02592-2
Häkkinen, S T, Nygren, H, Nohynek, L, Puupponen-Pimiä, R, Heiniö, R L, Maiorova, N, Rischer, H & Ritala, A 2020, ' Plant cell cultures as food—aspects of sustainability and safety ', Plant Cell Reports, vol. 39, no. 12, pp. 1655–1668 . https://doi.org/10.1007/s00299-020-02592-2
Key message Sustainability and safety aspects of plant cell cultures as food are presented. Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown. Abstract Biotechnologically produced cellul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aacdc1ce6e06d48896113cc61641cff1
http://europepmc.org/articles/PMC7644541
http://europepmc.org/articles/PMC7644541
Autor:
Takehiko Wada, Tetsuhiro Tanaka, Masaomi Nangaku, Moin A. Saleem, Hiroshi Kimura, Teruo Fujii, Reiko Inagi, Keiju Hiromura, Doi Kotaro
Publikováno v:
Doi, K, Kimura, H, Wada, T, Tanaka, T, Hiromura, K, Saleem, M A, Inagi, R, Nangaku, M & Fujii, T 2021, ' A novel method for successful induction of interdigitating process formation in conditionally immortalized podocytes from mice, rats, and humans ', Biochemical and Biophysical Research Communications, vol. 570, pp. 47-52 . https://doi.org/10.1016/j.bbrc.2021.07.029
Formation of processes in podocytes is regarded as the hallmark of maturity and normal physical condition for the cell. There are many accumulated findings about molecular mechanisms that cause retraction of podocyte processes; however, there is litt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f5f070ce1996d18f4e4157b94108118
https://pubmed.ncbi.nlm.nih.gov/34271436
https://pubmed.ncbi.nlm.nih.gov/34271436
Autor:
Hans Clevers, Esmée J. van Vliet, Anne C. Rios, Jane E. Visvader, Sylvia F. Boj, Carol Piani, Heggert G Rebel, Oded Kopper, Johanna F. Dekkers, Carla S Verissimo, Jennifer M. Rosenbluth, Norman Sachs, Joan S. Brugge, Ellen J. Wehrens
Publikováno v:
Nat Protoc
Nature Protocols, 16(4), 1936-1965. Nature Publishing Group
Nature Protocols, 16(4), 1936-1965. Nature Publishing Group
Organoid technology has revolutionized the study of human organ development, disease and therapy response tailored to the individual. Although detailed protocols are available for the generation and long-term propagation of human organoids from vario
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40bc869d4deafc27f9ee422c5e8cc7bf
https://europepmc.org/articles/PMC8221035/
https://europepmc.org/articles/PMC8221035/
Autor:
Barbara Korzeniowska, Krzysztof Wrzesinski, Chrisna Gouws, Stephen J. Fey, Carlemi Calitz, Helle Sedighi Frandsen
Publikováno v:
Methods in Molecular Biology ISBN: 9781071612453
Wrzesinski, K, Frandsen, H S, Calitz, C, Gouws, C, Korzeniowska, B & Fey, S J 2021, Clinostat 3D Cell Culture : Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids . in T A L Brevini, A Fazeli & K Turksen (eds), Next Generation Culture Platforms for Reliable In Vitro Models : Methods and Protocols . Humana Press, Methods in Molecular Biology, vol. 2273, pp. 17-62 . https://doi.org/10.1007/978-1-0716-1246-0_2
Wrzesinski, K, Frandsen, H S, Calitz, C, Gouws, C, Korzeniowska, B & Fey, S J 2021, Clinostat 3D Cell Culture : Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids . in T A L Brevini, A Fazeli & K Turksen (eds), Next Generation Culture Platforms for Reliable In Vitro Models : Methods and Protocols . Humana Press, Methods in Molecular Biology, vol. 2273, pp. 17-62 . https://doi.org/10.1007/978-1-0716-1246-0_2
Growing cells as 3D structures need not be difficult. Often, it is not necessary to change cell type, additives or growth media used. All that needs to be changed is the geometry: cells (whether primary, induced pluripotent, transformed or immortal)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c0ab0bdffa4401be20952ab1b3b16ef
https://doi.org/10.1007/978-1-0716-1246-0_2
https://doi.org/10.1007/978-1-0716-1246-0_2
Autor:
Calandrini, Camilla, Schutgens, Frans, Oka, Rurika, Margaritis, Thanasis, Candelli, Tito, Mathijsen, Luka, Ammerlaan, Carola, van Ineveld, Ravian L, Derakhshan, Sepide, de Haan, Sanne, Dolman, Emmy, Lijnzaad, Philip, Custers, Lars, Begthel, Harry, Kerstens, Hindrik H D, Visser, Lindy L, Rookmaaker, Maarten, Verhaar, Marianne, Tytgat, Godelieve A M, Kemmeren, Patrick, de Krijger, Ronald R, Al-Saadi, Reem, Pritchard-Jones, Kathy, Kool, Marcel, Rios, Anne C, van den Heuvel-Eibrink, Marry M, Molenaar, Jan J, van Boxtel, Ruben, Holstege, Frank C P, Clevers, Hans, Drost, Jarno, UU BETA RESEARCH, Faculteit Diergeneeskunde
Publikováno v:
Nature Communications, 11(1). Nature Publishing Group
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
Nature communications, 11(1):1310. Nature Publishing Group
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
Nature communications, 11(1):1310. Nature Publishing Group
Nature Communications
Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::908c9651a65106083bd59aef2388f9dc
https://doi.org/10.1038/s41467-020-15155-6
https://doi.org/10.1038/s41467-020-15155-6
Autor:
Gerrit R. J. Melles, Alina Miron, Silke Oellerich, Sorcha Ní Dhubhghaill, Daniele Spinozzi, Jessica T. Lie
Publikováno v:
Current eye research
Current Eye Research. TAYLOR & FRANCIS INC
Current Eye Research
Current Eye Research. TAYLOR & FRANCIS INC
Current Eye Research
Aim Studying cell migration of corneal endothelial cellsin vitrois challenging because the capacity for cell migration needs to be maintained while at the same time the tissue must remain fixed on a rigid substrate. In this study, we report a thermor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c6ec8ac0562691a65aca93d97fa3fbe
https://hdl.handle.net/10067/1712040151162165141
https://hdl.handle.net/10067/1712040151162165141
Autor:
Yehuda G. Assaraf, Luca Andrei, Mónica Suárez Korsnes, Radka Vaclavikova, Ignacio Ochoa Garrido, Tijana Stanković, Sandor Kasas, Milica Pešić
Publikováno v:
Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname
Drug Resistance Updates
Drug resistance updates, vol. 48, pp. 100658
Zaguán: Repositorio Digital de la Universidad de Zaragoza
Universidad de Zaragoza
instname
Drug Resistance Updates
Drug resistance updates, vol. 48, pp. 100658
Zaguán: Repositorio Digital de la Universidad de Zaragoza
Universidad de Zaragoza
The complexity of cancer biology and its clinical manifestation are driven by genetic, epigenetic, transcriptomic, proteomic and metabolomic alterations, supported by genomic instability as well as by environmental conditions and lifestyle factors. A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f964cac0c4545e6fbfbd29e9725476cc
http://zaguan.unizar.es/record/95733
http://zaguan.unizar.es/record/95733