Sequencing of human genomes extracted from single cancer cells isolated in a valveless microfluidic device

Autor: Loïc Baerlocher, Kalim U. Mir, Kamila Koprowska, Neil Ashley, Julien Schira, Niels Agersnap, Rodolphe Marie, Roland C. M. Vulders, Brian Bilenberg, Marie Pødenphant, F van Hemert, Celine Sabatel, Dianne Arnoldina Margaretha Wilhelmina Van Strijp, P. J. van der Zaag, Walter F. Bodmer, Jennifer L. Wilding, Tom Olesen, Simon J. McGowan, Anders Kristensen

Publikováno v: Marie, R, Pødenphant, M, Koprowska, K, Baerlocher, L, Vulders, R C M, Wilding, J, Ashley, N, McGowan, S J, van Strijp, D, van Hemert, F, Olesen, T, Agersnap, N, Bilenberg, B, Sabatel, C, Schira, J, Kristensen, A, Bodmer, W, van der Zaag, P J & Mir, K U 2018, ' Sequencing of human genomes extracted from single cancer cells isolated in a valveless microfluidic device ', Lab on a Chip, vol. 18, no. 13, pp. 1891-1902 . https://doi.org/10.1039/c8lc00169c

Sequencing the genomes of individual cells enables the direct determination of genetic heterogeneity amongst cells within a population. We have developed an injection-moulded valveless microfluidic device in which single cells from colorectal cancer

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f7e6691a030b9d8c90184143e704672
https://ora.ox.ac.uk/objects/uuid:f2d51e3c-1414-41b8-8d0f-f0639fb81122

Sequencing Metrics of Human Genomes Extracted from Single Cancer Cells Individually Isolated in a Valveless Microfluidic Device

Autor: Anders Kristensen, Vulders Rcm., Jennifer L. Wilding, Walter F. Bodmer, Marie Pødenphant, Niels Agersnap, Loïc Baerlocher, Rodolphe Marie, Brian Bilenberg, Celine Sabatel, Dianne Arnoldina Margaretha Wilhelmina Van Strijp, F van Hemert, Julien Schira, P. J. van der Zaag, Tom Olesen, Kalim U. Mir, Simon J. McGowan, Kamila Koprowska, Neil Ashley

Sequencing the genomes of individual cells enables the direct determination of genetic heterogeneity amongst cells within a population. We have developed an injection-moulded valveless microfluidic device in which single cells from colorectal cell (L

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d27617dc0ae74bfdb48d5ebeb86250b5
https://doi.org/10.1101/258780

Single-molecule DNA-mapping and whole-genome sequencing of individual cells

Autor: Henrik Flyvbjerg, Maksim Zalkovskij, Anders Kristensen, Andrej Mironov, Marie Pødenphant, Kalim U. Mir, Kamila Koprowska, Jonas Nyvold Pedersen, Neil Ashley, Rodolphe Marie, Brian Bilenberg, Loïc Baerlocher, Celine Sabatel, Walter F. Bodmer

Publikováno v: Marie, R, Pedersen, J N, Bærlocher, L, Koprowska, K, Pødenphant, M, Sabatel, C, Zalkovskij, M, Mironov, A, Bilenberg, B, Ashley, N, Flyvbjerg, H, Bodmer, W F, Kristensen, A & Mir, K U 2018, ' Single-molecule DNA-mapping and whole-genome sequencing of individual cells ', Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 44, pp. 11192-11197 . https://doi.org/10.1073/pnas.1804194115

To elucidate cellular diversity and clonal evolution in tissues and tumors, one must resolve genomic heterogeneity in single cells. To this end, we have developed low-cost, mass-producible micro-/nanofluidic chips for DNA extraction from individual c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4bc4340ac3b54827845c1359afef95b
https://orbit.dtu.dk/en/publications/9ae9c25d-b5e1-4368-a4b7-39062cb7fb5c

Successful implementation of ChIP-seq antibody quality control at Diagenode using automated ChIP protocol on the SX-8G IP-Star® Compact

Autor: Dominique Poncelet, Jan Hendrickx, Miklos Laczik, Catherine D’andrea, Géraldine Goens, Geoffrey Berguet, Ignacio Mazon, Celine Sabatel, Sharon Squazzo

Publikováno v: Epigenetics & Chromatin

Chromatin immunoprecipitation (ChIP) is the most widely used method to study protein-DNA interactions. A successful ChIP, however, is largely depending on the use of well characterized, highly specific ChIP-grade antibodies. ChIP-seq has become the g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53c22c185c34ba3935d50578017228bc
http://europepmc.org/articles/PMC3620703

MicroRNA-21 exhibits antiangiogenic function by targeting RhoB expression in endothelial cells.

Autor: Céline Sabatel, Ludovic Malvaux, Nicolas Bovy, Christophe Deroanne, Vincent Lambert, Maria-Luz Alvarez Gonzalez, Alain Colige, Jean-Marie Rakic, Agnès Noël, Joseph A Martial, Ingrid Struman

Publikováno v: PLoS ONE, Vol 6, Iss 2, p e16979 (2011)

BackgroundMicroRNAs (miRNAs) are endogenously expressed small non-coding RNAs that regulate gene expression at post-transcriptional level. The recent discovery of the involvement of these RNAs in the control of angiogenesis renders them very attracti

Externí odkaz: https://doaj.org/article/5f170ec679c140159250db094cff66eb