Patterns of Protein Synthesis and Tolerance of Anoxia in Root Tips of Maize Seedlings Acclimated to a Low-Oxygen Environment, and Identification of Proteins by Mass Spectrometry

Autor: Lan Huang, Min Shen, Alma L. Burlingame, William W.P. Chang, Justin K. M. Roberts, Cecelia Webster

Publikováno v: Plant Physiology. 122:295-318

Tolerance of anoxia in maize root tips is greatly improved when seedlings are pretreated with 2 to 4 h of hypoxia. We describe the patterns of protein synthesis during hypoxic acclimation and anoxia. We quantified the incorporation of [35S]methionine

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a741afd8badd9ff6ced2cb1dd79ec3b
https://doi.org/10.1104/pp.122.2.295

Analysis of nucleoside-binding proteins by ligand-specific elution from dye resin: application to Mycobacterium tuberculosis aldehyde dehydrogenases

Autor: Jin Ho Moon, Cecelia Webster, Emily Alipio Lyon, Li-Wei Hung, Justin K. M. Roberts, Chang-Yub Kim, Heungbok Kim, Thomas C. Terwilliger, Minmin Yu

Publikováno v: Kim, Chang-Yub; Webster, Cecelia; Roberts, Justin K.; Moon, Jin Ho; Alipio Lyon, Emily Z.; Kim, Heungbok; et al.(2009). Analysis of nucleoside-binding proteins by ligand-specific elution from dye resin: application to Mycobacterium tuberculosis aldehyde dehydrogenases. Journal of Structural and Functional Genomics, 10(4), pp 291-301. doi: 10.1007/s10969-009-9073-z. Retrieved from: http://www.escholarship.org/uc/item/9w05r8qr
Journal of Structural and Functional Genomics

We show that Cibacron Blue F3GA dye resin chromatography can be used to identify ligands that specifically interact with proteins from Mycobacterium tuberculosis, and that the identification of these ligands can facilitate structure determination by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a65a1f45c67a4d209df74af69e5968f6
http://www.escholarship.org/uc/item/9w05r8qr

Elongation and Termination Reactions of Protein Synthesis on Maize Root Tip Polyribosomes Studied in a Homologous Cell-Free System

Autor: Chang-Yub Kim, Justin K. M. Roberts, Cecelia Webster

Publikováno v: Plant Physiology. 96:418-425

We show that the control of gene expression at the level of elongation and termination of protein synthesis can be observed in vitro. Free cytoplasmic polyribosomes were isolated from maize (Zea mays) root tips, and translated in root tip extracts th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6aa1ed8d1437cc6a17094d146e669a5
https://doi.org/10.1104/pp.96.2.418

Contribution of Malate and Amino Acid Metabolism to Cytoplasmic pH Regulation in Hypoxic Maize Root Tips Studied Using Nuclear Magnetic Resonance Spectroscopy

Autor: Mark A. Hooks, Shaune Edwards, Justin K. M. Roberts, Cecelia Webster, Aaron P. Miaullis

Publikováno v: Plant physiology. 98(2)

(31)P-, (13)C-, and (15)N-nuclear magnetic resonance spectroscopy were used to determine the roles of malate, succinate, Ala, Asp, Glu, Gln, and gamma-aminobutyrate (GABA) in the energy metabolism and regulation of cytoplasmic pH in hypoxic maize (Ze

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cceb8a40cdc91be54f942c0d00b83bc5
https://pubmed.ncbi.nlm.nih.gov/16668665

Accelerated age-related decline in replicative life-span of Duchenne muscular dystrophy myoblasts: implications for cell and gene therapy

Autor: Helen M. Blau, Cecelia Webster

Publikováno v: Somatic cell and molecular genetics. 16(6)

An assessment of the replicative life-span of myoblasts is of fundamental importance in designing treatment strategies for Duchenne muscular dystrophy (DMD) based on cell or gene therapy. To ascertain myoblast life-span, or the total number of cell d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8b6aa261e79e22b246280426d78d97d
https://pubmed.ncbi.nlm.nih.gov/2267630