Substrate binding in the processive cellulase Cel7A: Transition state of complexation and roles of conserved tryptophan residues

Autor: Røjel, Nanna, Kari, Jeppe, Sørensen, Trine Holst, Badino, Silke F., Morth, J. Preben, Schaller, Kay, Cavaleiro, Ana Mafalda, Borch, Kim, Westh, Peter

Publikováno v: Røjel, N, Kari, J, Sørensen, T H, Badino, S F, Morth, J P, Schaller, K, Cavaleiro, A M, Borch, K & Westh, P 2020, ' Substrate binding in the processive cellulase Cel7A: Transition state of complexation and roles of conserved tryptophan residues ', Journal of Biological Chemistry, vol. 295, no. 6, pp. 1454-1463 . https://doi.org/10.1074/jbc.ra119.011420

Cellobiohydrolases effectively degrade cellulose and are of biotechnological interest because they can convert lignocellulosic biomass to fermentable sugars. Here, we implemented a fluorescence-based method for real-time measurements of complexation

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https://orbit.dtu.dk/en/publications/ae3f42d8-1fde-40b2-ae95-53499ef22985

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An expression tag toolbox for microbial production of membrane bound plant cytochromes P450

Autor: Vazquez-Albacete, Dario, Cavaleiro, Ana Mafalda, Christensen, Ulla, Seppälä, Susanna, Møller, Birger Lindberg, Nørholm, Morten H.H.

Publikováno v: Vazquez Albacete, D, Cavaleiro, M, Christensen, U, Seppala, S, Møller, B L & Nørholm, M 2017, ' An expression tag toolbox for microbial production of membrane bound plant cytochromes P450 ', Biotechnology and Bioengineering, vol. 114, no. 4, pp. 751-760 . https://doi.org/10.1002/bit.26203
Vazquez-Albacete, D, Cavaleiro, A M, Christensen, U, Seppälä, S, Møller, B L & Nørholm, M H H 2017, ' An expression tag toolbox for microbial production of membrane bound plant cytochromes P450 ', Biotechnology and Bioengineering, vol. 114, no. 4, pp. 751-760 . https://doi.org/10.1002/bit.26203

Membrane-associated Cytochromes P450 (P450s) are one of the most important enzyme families for biosynthesis of plant-derived medicinal compounds. However, the hydrophobic nature of P450s makes their use in robust cell factories a challenge. Here we e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::1ab13dea475d31e1c21a321d41d5405a
https://orbit.dtu.dk/en/publications/acb6c990-8e00-440d-830d-fa3008334388

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Uracil Excision for Assembly of Complex Pathways.

Autor: Cavaleiro, Ana Mafalda, Nielsen, Morten T., Kim, Se Hyeuk, Seppälä, Susanna, Nørholm, Morten H. H.

Publikováno v: Hydrocarbon & Lipid Microbiology Protocols; 2016, p27-37, 11p