A low complexity rapid molecular method for detection of Clostridium difficile in stool.

Autor: Cathal J McElgunn, Clint R Pereira, Nicholas J Parham, James E Smythe, Michael J Wigglesworth, Anna Smielewska, Surendra A Parmar, Olga A Gandelman, Nicholas M Brown, Laurence C Tisi, Martin D Curran

Publikováno v: PLoS ONE, Vol 9, Iss 1, p e83808 (2014)

Here we describe a method for the detection of Clostridium difficile from stool using a novel low-complexity and rapid extraction process called Heat Elution (HE). The HE method is two-step and takes just 10 minutes, no specialist instruments are req

Externí odkaz: https://doaj.org/article/f4f54aff9b144abeb7f26b1d0236a393

A comprehensive analysis of loss of heterozygosity caused by hemizygous deletions in renal cell carcinoma using a subtraction library

Autor: Cathal J. McElgunn, Motowo Nakajima, Naoko S. Nishikawa, Shubhashish Sarkar, Kazuo Gohiji, Naoya Hatano, Yasuhiko Shibanaka, Ryoiti Kiyama, Kazuo Ozawa

Publikováno v: Molecular Carcinogenesis. 31:161-170

Several new loci were identified by a comprehensive analysis of loss of heterozygosity (LOH) using a subtraction library between matched normal and renal cell carcinoma (RCC) tissues. A total of 187 clones from the library, with a complexity of 1x10(

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d77e9866ea72122a5fdc1ba2d9ec0f7
https://doi.org/10.1002/mc.1051

Treatment with an anti-CD14 monoclonal antibody delays and inhibits lipopolysaccharide-induced gene expression in humans in vivo

Autor: C Arnold, Spek, Annelies, Verbon, Hella, Aberson, John P, Pribble, Cathal J, McElgunn, Terence, Turner, Tim, Axtelle, Jan, Schouten, Tom, Van Der Poll, Pieter H, Reitsma

Publikováno v: Journal of clinical immunology. 23(2)

CD14 is a receptor important for activation of cells by lipopolysaccharide (LPS). Treatment with the CD14 antibody IC14 was previously found to attenuate the release of proinflammatory cytokines and some chemokines into the circulation of healthy hum

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::0ffda63af65d18beb75f2aebc034c4cc
https://pubmed.ncbi.nlm.nih.gov/12757265

Genomic deletions of MSH2 and MLH1 in colorectal cancer families detected by a novel mutation detection approach

Autor: Johan J.P. Gille, Charlotte J. Dommering, Jan P. Schouten, D. de Jong, Cathal J. McElgunn, J Th Wijnen, R J van Schooten, Gerard Pals, Gerrit A. Meijer, Fred H. Menko, M. E. Craanen, Petra M. Nederlof, Frans B. L. Hogervorst

Publikováno v: Gille, J J P, Hogervorst, F B L, Pals, G, Wijnen, J T, van Schooten, R J, Dommering, C J, Meijer, G A, Craanen, M E, Nederlof, P M, de Jong, D, McElgunn, C J, Schouten, J P & Menko, F H 2002, ' Genomic deletions of MSH2 and MLH1 in colorectal cancer families detected by a novel mutation detection approach ', British Journal of Cancer, vol. 87, no. 8, pp. 892-7 . https://doi.org/10.1038/sj.bjc.6600565
British Journal of Cancer, 87(8), 892-7. Nature Publishing Group
British Journal of Cancer

Hereditary non-polyposis colorectal cancer is an autosomal dominant condition due to germline mutations in DNA-mismatch-repair genes, in particular MLH1, MSH2 and MSH6. Here we describe the application of a novel technique for the detection of genomi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::030f63456ca93d005265c710071d90a9
https://research.vumc.nl/en/publications/ffbcdafb-f407-4cf3-937d-1d8b8a2640c1

Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification

Autor: Danny A. Zwijnenburg, Raymond Waaijer, Filip Diepvens, Gerard Pals, Jan P. Schouten, Cathal J. McElgunn

Publikováno v: Nucleic acids research. 30(12)

We describe a new method for relative quantification of 40 different DNA sequences in an easy to perform reaction requiring only 20 ng of human DNA. Applications shown of this multiplex ligation-dependent probe amplification (MLPA) technique include

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e7f77434027a3b032bcc93065618adb
https://pubmed.ncbi.nlm.nih.gov/12060695