Expression in Escherichia coli and Purification of Microbial Enzymes for the Production of Enzymatic Analytical Kits to Measure Relevant Metabolites

Autor: José A. M. Prates, André Fernandes, Catarina I. P. D. Guerreiro, Vânia O. Fernandes, Carlos M. G. A. Fontes, Luís M. A. Ferreira, P I P Ponte

Publikováno v: Journal of Applied Life Sciences International. 2:1-8

Aim: The present study describes the procedure for the isolation, cloning, expression and purification of eight different microbial enzymes required for the assembly of a comprehensive range of enzymatic test kits. Methodology: The genes encoding the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::84405ff55ca11beda142990044b4bad5
https://doi.org/10.9734/jalsi/2015/13113

A Novel Platform for High-Throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli

Autor: Renaud Vincentelli, Joana L. A. Brás, Ana Filipa Sequeira, Catarina I. P. D. Guerreiro, Carlos M. G. A. Fontes, Vânia O. Fernandes

Publikováno v: Methods in Molecular Biology ISBN: 9781493970599

Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is quickly replacing the classical cloning and mutagenesis procedures and allows generating nu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d4dbdee2ed167e78ade03ff05a90bbcf
https://doi.org/10.1007/978-1-4939-7060-5_7

T7 Endonuclease I Mediates Error Correction in Artificial Gene Synthesis

Autor: Carlos M. G. A. Fontes, Catarina I. P. D. Guerreiro, Renaud Vincentelli, Ana Filipa Sequeira

Publikováno v: Molecular Biotechnology
Molecular Biotechnology, Humana Press, 2016, 58 (8-9), pp.573-584. ⟨10.1007/s12033-016-9957-7⟩
Molecular Biotechnology, 2016, 58 (8-9), pp.573-584. ⟨10.1007/s12033-016-9957-7⟩

Efficacy of de novo gene synthesis largely depends on the quality of overlapping oligonucleotides used as template for PCR assembly. The error rate associated with current gene synthesis protocols limits the efficient and accurate production of synth

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76c1c7065bc1d4f6af3ca78a8c985a67
https://hal.archives-ouvertes.fr/hal-01439106

Role of a family 11 carbohydrate-binding module in the function of a recombinant cellulase used to supplement a barley-based diet for broiler chickens

Autor: P I P Ponte, Tiago Ribeiro, M.M. Lordelo, L. Falcão, Catarina I. P. D. Guerreiro, Carlos M. G. A. Fontes, José A. M. Prates, L M A Ferreira, Joao P.B. Freire

Publikováno v: British Poultry Science. 49:446-454

1. Cellulases and xylanases display a modular architecture that comprises a catalytic module linked to one or more non-catalytic carbohydrate-binding modules (CBMs). CBMs have been classified into 52 different families, based on primary structure sim

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a96938f0101c66c6428e14342d1eba65
https://doi.org/10.1080/00071660802216676

Crystal Structures of Clostridium thermocellum Xyloglucanase, XGH74A, Reveal the Structural Basis for Xyloglucan Recognition and Degradation

Autor: Harry Brumer, Luís M. A. Ferreira, Martin J. Baumann, Edward J. Taylor, Gideon J. Davies, Carlos Martinez-Fleites, José A. M. Prates, Catarina I. P. D. Guerreiro, Carlos M. G. A. Fontes

Publikováno v: Journal of Biological Chemistry. 281:24922-24933

The enzymatic degradation of the plant cell wall is central both to the natural carbon cycle and, increasingly, to environmentally friendly routes to biomass conversion, including the production of biofuels. The plant cell wall is a complex composite

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04920f47c399b088ac756c2b689aa5d2
https://doi.org/10.1074/jbc.m603583200