Cryopreservation of human pluripotent stem cell-derived cardiomyocytes is not detrimental to their molecular and functional properties

Autor: Lettine van den Brink, Karina O. Brandão, Loukia Yiangou, Mervyn P.H. Mol, Catarina Grandela, Christine L. Mummery, Arie O. Verkerk, Richard P. Davis

Publikováno v: Stem Cell Research, Vol 43, Iss , Pp - (2020)

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have emerged as a powerful platform for in vitro modelling of cardiac diseases, safety pharmacology and drug screening. All these applications require large quantities of well-cha

Externí odkaz: https://doaj.org/article/6ecd8b74d88648a086ec4807df04f6b8

STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells

Autor: Albert Blanch-Asensio, Catarina Grandela, Karina O. Brandão, Tessa de Korte, Hailiang Mei, Yavuz Ariyurek, Loukia Yiangou, Mervyn P.H. Mol, Berend J. van Meer, Susan L. Kloet, Christine L. Mummery, Richard P. Davis

Publikováno v: None
Cell Reports Methods, 2(10):100300. Cell Press
Cell Reports Methods

Inserting large DNA payloads (>10 kb) into specific genomic sites of mammalian cells remains challenging. Applications ranging from synthetic biology to evaluating the pathogenicity of disease-associated variants for precision medicine initiatives wo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44b042f10617490839f02a85476b0bc7
http://hdl.handle.net/1887/3564512

CRISPR/Cas9-Mediated Introduction of Specific Heterozygous Mutations in Human Induced Pluripotent Stem Cells

Autor: Catarina Grandela, Loukia Yiangou, Richard P. Davis, Karina de Oliveira Brandão, Christine L. Mummery

Publikováno v: Methods in Molecular Biology
Methods Mol Biol
Methods in Molecular Biology ISBN: 9781071621189

Advances in genome editing and our ability to derive and differentiate human induced pluripotent stem cells (hiPSCs) into a wide variety of cell types present in the body is revolutionizing how we model human diseases in vitro. Central to this has be

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09e7f36135fbd92345b512b8b27af95a

Cryopreservation of human pluripotent stem cell-derived cardiomyocytes is not detrimental to their molecular and functional properties

Autor: Richard P. Davis, Loukia Yiangou, Arie O. Verkerk, Catarina Grandela, Christine L. Mummery, Mervyn P.H. Mol, Karina de Oliveira Brandão, Lettine van den Brink

Publikováno v: Stem Cell Research
Stem cell research
Stem Cell Research, Vol 43, Iss, Pp-(2020)
Stem Cell Research, 43. ELSEVIER
Stem cell research, 43:101698. Elsevier

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have emerged as a powerful platform for in vitro modelling of cardiac diseases, safety pharmacology and drug screening. All these applications require large quantities of well-cha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1d51b48ab2ccc086ba57ebb3e2d9b02

Isogenic sets of hiPSC-CMs harboring KCNH2 mutations capture location-related phenotypic differences

Autor: Christine L. Mummery, Leon G.J. Tertoolen, Richard P. Davis, Arie O. Verkerk, Luca Sala, Catarina Grandela, Duncan C. Miller, Karina de Oliveira Brandão, Berend J. van Meer, Mervyn P.H. Mol, Lettine van den Brink

Publikováno v: BioRxiv
bioRxiv

AimsLong QT syndrome type 2 (LQT2) is caused by mutations in the geneKCNH2, encoding the hERG ion channel. Clinically, mild and severe phenotypes are associated with this cardiac channelopathy, complicating efforts to predict patient risk. The locati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bc411b6c5954fe2c60ee75cc1c37580