Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Cassandra L Crihfield"'
Publikováno v:
Analytical and Bioanalytical Chemistry. 414:713-720
The topology of DNA is a critical quality attribute for plasmid-based pharmaceuticals, making quantification of trace levels of plasmid topoisomers an important analytical priority. An automated and cost-effective method based on capillary gel electr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f384909fef2a3160f8844a25abc9bd10
https://doi.org/10.1007/s00216-021-03714-9
https://doi.org/10.1007/s00216-021-03714-9
Publikováno v:
Analytical Chemistry. 93:1537-1543
Protein sieving, which is a fundamental tool in the biotechnology field, can be automated using capillary gel electrophoresis. The high-viscosity and biocompatible linear gels required for capillary sieving must be replaced for each run using high pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9176a9b3a998b069e39b10888de357ae
https://doi.org/10.1021/acs.analchem.0c03865
https://doi.org/10.1021/acs.analchem.0c03865
Publikováno v:
Analytical and bioanalytical chemistry. 414(1)
The topology of DNA is a critical quality attribute for plasmid-based pharmaceuticals, making quantification of trace levels of plasmid topoisomers an important analytical priority. An automated and cost-effective method based on capillary gel electr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6e6d46da406c47b08cc197d7107594db
https://pubmed.ncbi.nlm.nih.gov/34693472
https://pubmed.ncbi.nlm.nih.gov/34693472
Autor:
Srikanth Gattu, Lloyd Bwanali, Lindsay M. Veltri, Grace Lu, Cassandra L. Crihfield, Lisa A. Holland
Publikováno v:
Methods. 146:93-106
Capillary electrophoresis provides a rapid, cost-effective platform for enzyme and substrate characterization. The high resolution achievable by capillary electrophoresis enables the analysis of substrates and products that are indistinguishable by s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42540d1dc7537e870dd5c49ede32320c
https://doi.org/10.1016/j.ymeth.2018.02.005
https://doi.org/10.1016/j.ymeth.2018.02.005
Publikováno v:
Chemical Reviews. 118:7867-7885
Capillary electrophoresis has emerged as a powerful approach for carbohydrate analyses since 2014. The method provides high resolution capable of separating carbohydrates by charge-to-size ratio. Principle applications are heavily focused on N-glycan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea95952d16868f943bc89b7db26ac8c7
https://doi.org/10.1021/acs.chemrev.7b00669
https://doi.org/10.1021/acs.chemrev.7b00669
Autor:
Srikanth Gattu, Cassandra L. Crihfield, Victoria R Zeger, Lisa A. Holland, Ebenezer O Newton, Lloyd Bwanali
Publikováno v:
Anal Chem
Sialylation and sialic acid linkage in N-glycans are markers of disease, but are analytically challenging to quantify. A capillary electrophoresis method is reported that integrates a unique combination of enzymes and lectins to modify sialylated N-g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1148140b52d20fd1054664158ce2466b
https://pubmed.ncbi.nlm.nih.gov/31829566
https://pubmed.ncbi.nlm.nih.gov/31829566
Autor:
C.A. Wilson, Lisa A. Holland, W.M. Penny, Cassandra L. Crihfield, Courtney J Kristoff, Lindsay M. Veltri
Publikováno v:
Data in Brief
Data in Brief, Vol 29, Iss, Pp-(2020)
Data in Brief, Vol 29, Iss, Pp-(2020)
Protein separations and analyses are fundamental to fields of study that include biochemistry, biology, physiology, drug discovery, pharmaceuticals, as well as agricultural and food based industries. Here, we provide the data from a novel phospholipi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ae7fca157de9628e1230925a8536ed0
https://pubmed.ncbi.nlm.nih.gov/32368572
https://pubmed.ncbi.nlm.nih.gov/32368572
Publikováno v:
Analytical Chemistry
Phospholipid nanogels enhance the stability and performance of the exoglycosidase enzyme neuraminidase and are used to create a fixed zone of enzyme within a capillary. With nanogels, there is no need to covalently immobilize the enzyme, as it is phy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2794e1b7b028081963acd6c2ee86dc3
http://europepmc.org/articles/PMC5214287
http://europepmc.org/articles/PMC5214287
Publikováno v:
Analytical and Bioanalytical Chemistry
This review of capillary electrophoresis methods for DNA analyses covers critical advances from 2009 to 2014, referencing 184 citations. Separation mechanisms based on free-zone capillary electrophoresis, Ogston sieving, and reptation are described.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc2a31db45aaf22be9feae2f8f84b4ce
http://europepmc.org/articles/PMC4551542
http://europepmc.org/articles/PMC4551542