Výsledky vyhledávání - "Carole A. Foy"

Akademický článek

Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR

Autor: Eloise Busby, Alexandra S. Whale, R. Bridget Ferns, Paul R. Grant, Gary Morley, Jonathan Campbell, Carole A. Foy, Eleni Nastouli, Jim F. Huggett, Jeremy A. Garson

Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)

Abtract Establishing a cure for HIV is hindered by the persistence of latently infected cells which constitute the viral reservoir. Real-time qPCR, used for quantification of this reservoir by measuring HIV DNA, requires external calibration; a commo

Externí odkaz: https://doaj.org/article/2b436614675045baa96789477b129f7b

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Akademický článek

Assessing the Accuracy of Quantitative Molecular Microbial Profiling

Autor: Denise M. O'Sullivan, Thomas Laver, Sasithon Temisak, Nicholas Redshaw, Kathryn A. Harris, Carole A. Foy, David J. Studholme, Jim F. Huggett

Publikováno v: International Journal of Molecular Sciences, Vol 15, Iss 11, Pp 21476-21491 (2014)

The application of high-throughput sequencing in profiling microbial communities is providing an unprecedented ability to investigate microbiomes. Such studies typically apply one of two methods: amplicon sequencing using PCR to target a conserved or

Externí odkaz: https://doaj.org/article/2500e7503ca74c8a9b989d4f405c41cd

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Akademický článek

A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability

Autor: Nicholas Redshaw, Timothy Wilkes, Alexandra Whale, Simon Cowen, Jim Huggett, Carole A. Foy

Publikováno v: BioTechniques, Vol 54, Iss 3, Pp 155-164 (2013)

MicroRNAs (miRNAs) are short (∼22 nucleotides), non-coding RNA molecules that post-transcriptionally regulate gene expression. As the miRNA field is still in its relative infancy, there is currently a lack of consensus regarding optimal methodologi

Externí odkaz: https://doaj.org/article/bc8a9dc8c3df40bab75e8f6ddc6374cb

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The Dangers of Using Cq to Quantify Nucleic Acid in Biological Samples: A Lesson From COVID-19

Publikováno v: CLINICAL CHEMISTRY

Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA quantities, measured by reverse transcription quantitative PCR (RT-qPCR), have been proposed to stratify clinical risk or determine analytical performance targets. We investi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c646f7cac7074510bd30c4ded8f875de
https://doi.org/10.1093/clinchem/hvab219

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Applicability of Control Materials To Support Gene Promoter Characterization and Expression in Engineered Cells Using Digital PCR

Autor: Ana Fernandez-Gonzalez, Simon Cowen, Juhyun Kim, Carole A. Foy, Jose Jimenez, Jim F. Huggett

The use of standardized components and processes in engineering underpins the design-build-test model, and the engineering of biological systems is no different. Substantial efforts to standardize both the components and the methods to validate the e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______2659::3a2b96fa4fd8a244bdb890f2a87c5cb9
https://zenodo.org/record/6652237

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A standardised methodology for the extraction and quantification of cell-free DNA in cerebrospinal fluid and application to evaluation of Alzheimer's disease and brain cancers

Autor: Petros Takousis, Alison S. Devonshire, Nicholas Redshaw, Louisa von Baumgarten, Alexandra S. Whale, Gerwyn M. Jones, Ana Fernandez-Gonzalez, Jan Martin, Carole A. Foy, Panagiotis Alexopoulos, Jim F. Huggett, Robert Perneczky

Publikováno v: New biotechnology 72, 97-106 (2022). doi:10.1016/j.nbt.2022.10.001

Cerebrospinal fluid (CSF) is a source of diagnostic biomarkers for a range of neurological conditions. Cell-free DNA (cfDNA) is detected in CSF and differences in the concentration of cell-free mitochondrial DNA have been reported in studies of neuro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca0fe30f754cc91b60066b47376c0ff7
https://pubmed.ncbi.nlm.nih.gov/36202346

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The performance of human cytomegalovirus digital PCR reference measurement procedure in seven external quality assessment schemes over four years

Autor: Denise M. O'Sullivan, Alexandra Bogožalec Košir, Alison S. Devonshire, Maria Karczmarczyk, Fran Van Heuverswyn, Jim F. Huggett, Annabell Plauth, Gerwyn M. Jones, Heinz Zeichhardt, Jörg Neukammer, Hans-Peter Grunert, Martin Kammel, Jannika Neeb, Carole A. Foy, Andreas Kummrow, Philippe Corbisier, Jernej Pavšič, Heinz Schimmel, Mojca Milavec

A candidate digital PCR (dPCR)-based reference measurement procedure for quantification of human cytomegalovirus (hCMV) was evaluated in 10 viral load comparison schemes (seven external quality assessment (EQA) and three additional training schemes)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2eb0a92008a28eb8a23aa5dce060e0dc
http://hdl.handle.net/1942/37662

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Pushing the Envelope with Clinical Use of Digital PCR

Autor: Jim F. Huggett, Simon Cowen, Alexandra S. Whale, Alison S. Devonshire, Carole A. Foy

Publikováno v: Clinical chemistry. 67(7)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e1ba6fe9c4ae916fbe5b22acff0f7cb
https://pubmed.ncbi.nlm.nih.gov/33842952

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Akademický článek

Methods for applying accurate digital PCR analysis on low copy DNA samples.

Autor: Alexandra S Whale, Simon Cowen, Carole A Foy, Jim F Huggett

Publikováno v: PLoS ONE, Vol 8, Iss 3, p e58177 (2013)

Digital PCR (dPCR) is a highly accurate molecular approach, capable of precise measurements, offering a number of unique opportunities. However, in its current format dPCR can be limited by the amount of sample that can be analysed and consequently a

Externí odkaz: https://doaj.org/article/ad51dffb2d314c27bd0dba1c220ec3ae

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International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant

Publikováno v: Whale, A S, Devonshire, A S, Karlin-Neumann, G, Regan, J, Javier, L, Cowen, S, Fernandez-Gonzalez, A, Jones, G M, Redshaw, N, Beck, J, Berger, A W, Combaret, V, Kjersgaard, N D, Davis, L, Fina, F, Forshew, T, Andersen, R F, Galbiati, S, Hernandez, A G, Haynes, C A, Janku, F, Lacave, R, Lee, J, Mistry, V, Pender, A, Pradines, A, Proudhon, C, Saal, L H, Stieglitz, E, Ulrich, B, Foy, C A, Parkes, H, Tzonev, S & Huggett, J F 2017, ' International interlaboratory digital PCR study demonstrating high reproducibility for the measurement of a rare sequence variant ', Analytical Chemistry, vol. 89, no. 3, pp. 1724-1733 . https://doi.org/10.1021/acs.analchem.6b03980

This study tested the claim that digital PCR (dPCR) can offer highly reproducible quantitative measurements in disparate laboratories. Twenty-one laboratories measured four blinded samples containing different quantities of a KRAS fragment encoding G

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebc01d24aa5634e6fdf1096bd5afb8ec
https://doi.org/10.1021/acs.analchem.6b03980

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