Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Carola Engler"'
Publikováno v:
PLoS ONE, Vol 6, Iss 2, p e16765 (2011)
The field of synthetic biology promises to revolutionize biotechnology through the design of organisms with novel phenotypes useful for medicine, agriculture and industry. However, a limiting factor is the ability of current methods to assemble compl
Externí odkaz:
https://doaj.org/article/3f13e5f5be6347998ec5644eed1d05e9
Publikováno v:
PLoS ONE, Vol 6, Iss 5, p e19722 (2011)
Generation of customized DNA binding domains targeting unique sequences in complex genomes is crucial for many biotechnological applications. The recently described DNA binding domain of the transcription activator-like effectors (TALEs) from Xanthom
Externí odkaz:
https://doaj.org/article/c54548b79ce44e05b14f695fea016465
Publikováno v:
PLoS ONE, Vol 6, Iss 6, p e20556 (2011)
We have developed an efficient strategy for cloning of PCR products that contain an unknown region flanked by a known sequence. As with ligation-independent cloning, the strategy is based on homology between sequences present in both the vector and t
Externí odkaz:
https://doaj.org/article/bf0e61fd7f0643f8abcb832c6abf4d9a
Publikováno v:
PLoS ONE, Vol 4, Iss 5, p e5553 (2009)
We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct. This protocol is based on the use of ty
Externí odkaz:
https://doaj.org/article/1ed45db2e77448e0907590df9cc03165
Publikováno v:
PLoS ONE, Vol 3, Iss 11, p e3647 (2008)
Current cloning technologies based on site-specific recombination are efficient, simple to use, and flexible, but have the drawback of leaving recombination site sequences in the final construct, adding an extra 8 to 13 amino acids to the expressed p
Externí odkaz:
https://doaj.org/article/40364dde0124458fa539f7047524a663
Autor:
Sylvestre Marillonnet, Nicola J. Patron, Tim-Martin Ehnert, Jonathan D. G. Jones, Ramona Gruetzner, Stefan Werner, Mark Youles, Carola Engler
Publikováno v:
ACS Synthetic Biology. 3:839-843
Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versa
Publikováno v:
Bioengineered. 3:38-43
Recent progress in the field of synthetic biology has led to the creation of cells containing synthetic genomes. Although these first synthetic organisms contained copies of natural genomes, future work will be directed toward engineering of organism
Autor:
Victor Klimyuk, Gerben van Eldik, Sylvestre Marillonnet, Anatoli Giritch, Yuri Gleba, Carola Engler, Johan Botterman
Publikováno v:
Proceedings of the National Academy of Sciences. 103:14701-14706
Plant viral vectors allow expression of heterologous proteins at high yields, but so far, they have been unable to express heterooligomeric proteins efficiently. We describe here a rapid and indefinitely scalable process for high-level expression of
Autor:
Sylvestre Marillonnet, Carola Engler
Publikováno v:
DNA Cloning and Assembly Methods ISBN: 9781627037631
DNA assembly methods are essential tools for biological research and biotechnology. Therefore various methods have been developed to clone DNA fragments of interest. Conventional methods usually require several cloning steps to generate a construct o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d07ddb8a3ee3564141d5f643af5e151f
https://doi.org/10.1007/978-1-62703-764-8_9
https://doi.org/10.1007/978-1-62703-764-8_9
Autor:
Carola, Engler, Sylvestre, Marillonnet
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1073
A basic requirement for synthetic biology is the availability of efficient DNA assembly methods. We have previously reported the development of Golden Gate cloning, a method that allows parallel assembly of multiple DNA fragments in a one-tube reacti