Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system

Autor: Remi L. Gratacap, Tim Regan, Carola E. Dehler, Samuel A. M. Martin, Pierre Boudinot, Bertrand Collet, Ross D. Houston

Publikováno v: BMC Biotechnology, Vol 20, Iss 1, Pp 1-9 (2020)

Abstract Background Genome editing is transforming bioscience research, but its application to non-model organisms, such as farmed animal species, requires optimisation. Salmonids are the most important aquaculture species by value, and improving gen

Externí odkaz: https://doaj.org/article/549342a1b6314e63a680d23a0b2c6d79

Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system

Autor: Bertrand Collet, Pierre Boudinot, Carola E. Dehler, Ross D. Houston, Remi L. Gratacap, Samuel A.M. Martin, Tim Regan

Publikováno v: BMC Biotechnology
BMC Biotechnology, BioMed Central, 2020, 20 (1), ⟨10.1186/s12896-020-00626-x⟩
Gratacap, R, Regan, T, Dehler, C E, Martin, S A M, Boudinot, P, Collet, B & Houston, R 2020, ' Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system ', BMC Biotechnology . https://doi.org/10.1186/s12896-020-00626-x
BMC Biotechnology, Vol 20, Iss 1, Pp 1-9 (2020)

Background Genome editing is transforming bioscience research, but its application to non-model organisms, such as farmed animal species, requires optimisation. Salmonids are the most important aquaculture species by value, and improving genetic resi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b459478d2f6322699f2b86b14cbb8d51
https://hal.inrae.fr/hal-03129758/file/2020_Gratacap_bmcbiotechnol.pdf

Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr (Salmo salar L.)

Autor: Samuel A.M. Martin, Carola E. Dehler, Christopher J. Secombes

Publikováno v: Aquaculture. 467:149-157

Gut microbes are key players in host immune system priming, protection and development, as well as providing nutrients to the host that would be otherwise unavailable. Due to this importance, studies investigating the link between host and microbe ar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e9ef8bef9cda4e04ae83ef1a3a13b21
https://doi.org/10.1016/j.aquaculture.2016.07.017

Viral resistance and IFN signaling in STAT2 knockout fish cells

Autor: Jun Zou, Armel Houel, Giulia Della Pelle, Tatiana Dovgan, Radek Machat, Katherine Lester, Catherine Collins, Bertrand Collet, Carola E. Dehler, Luc Jouneau, Pierre Boudinot, Samuel A.M. Martin

Publikováno v: Journal of Immunology
Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2019, 203 (2), pp.465-475. ⟨10.4049/jimmunol.1801376⟩
The Journal of Immunology Author Choice

Key Points A stat2−/− Oncorhynchus tshawytscha cell line was isolated. STAT2 is required for type I but not type II signaling in salmonid cells.
IFN belong to a group of cytokines specialized in the immunity to viruses. Upon viral infection,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0905dc183c3a8cf8ca368cb34b95ea53
https://hal.inrae.fr/hal-02622473

Phylogeny and expression of the tetraspanin CD9 in salmonid cell lines in response to interferon stimulation

Autor: B. Collet, Samuel A.M. Martin, Pierre Boudinot, Carola E. Dehler

Publikováno v: Fish & Shellfish Immunology
Fish & Shellfish Immunology, 2019, 91, pp.433. ⟨10.1016/j.fsi.2019.04.186⟩

International audience

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::484bb6603aa9f53b6f909be56a39cf8a
https://doi.org/10.1016/j.fsi.2019.04.186