Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Carola Doce"'
Autor:
Carola Doce, Christian Fufezan, Marcus Bantscheff, Nico Zinn, Christine Boecker, Toby Mathieson, Thilo Werner, Gavain Sweetman
Publikováno v:
Journal of proteome research. 20(3)
Multiplexed quantitative proteomics enabled complex workflows to study the mechanisms by which small molecule drugs interact with the proteome such as thermal proteome profiling (TPP) or multiplexed proteome dynamics profiling (mPDP). TPP measures ch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d356ea7731030a4d4bad0a79b52cf30c
https://pubmed.ncbi.nlm.nih.gov/33621079
https://pubmed.ncbi.nlm.nih.gov/33621079
Autor:
Marcel Paulmann, Gerard Joberty, Aaron T. Cheng, Maria Fälth-Savitski, Paola Grandi, Carola Doce, Markus Bösche, Gerard Drewes
Publikováno v:
The CRISPR Journal
CRISPR/Cas9–based gene knockouts (KOs) enable precise perturbation of target gene function in human cells, which is ideally assessed in an unbiased fashion by molecular omics readouts. Typically, this requires the lengthy process of isolating KO su
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ae66ba927f026ca42c5d4723f0aeba3
https://pubmed.ncbi.nlm.nih.gov/32315231
https://pubmed.ncbi.nlm.nih.gov/32315231
Autor:
Holger Franken, Dirk Eberhard, Marcus Bantscheff, Mikhail M. Savitski, Friedrich B M Reinhard, Wolfgang Huber, Dorothee Childs, Thilo Werner, Maria Fälth Savitski, Carola Doce, Gerard Drewes
Publikováno v:
Nature Methods. 12:1129-1131
We extended thermal proteome profiling to detect transmembrane protein-small molecule interactions in cultured human cells. When we assessed the effects of detergents on ATP-binding profiles, we observed shifts in denaturation temperature for ATP-bin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fcb9a1dc0e49393e87bdfe376c868c9c
https://doi.org/10.1038/nmeth.3652
https://doi.org/10.1038/nmeth.3652
Autor:
Thilo Werner, Isabelle Becher, Mikhail M. Savitski, Marcus Bantscheff, Gavain Sweetman, Carola Doce
Publikováno v:
Analytical Chemistry. 84:7188-7194
Isobaric mass tag-based quantitative proteomics strategies such as iTRAQ and TMT utilize reporter ions in the low-mass range of tandem MS spectra for relative quantification. The number of samples that can be compared in a single experiment (multiple
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a65158823d8d79fc2434c91c66357a2
https://doi.org/10.1021/ac301553x
https://doi.org/10.1021/ac301553x
Autor:
Marcus Bantscheff, Ina Tögel, Holger Franken, Thilo Werner, Wolfgang Huber, Dorothee Childs, Gerard Drewes, Stephan Gade, Toby Mathieson, Carola Doce, Gavain Sweetman, Mikhail M. Savitski, Friedrich B M Reinhard
Publikováno v:
Nature protocols. 10(10)
The direct detection of drug-protein interactions in living cells is a major challenge in drug discovery research. Recently, we introduced an approach termed thermal proteome profiling (TPP), which enables the monitoring of changes in protein thermal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43b66295e225640bdc595031cda30c77
https://pubmed.ncbi.nlm.nih.gov/26379230
https://pubmed.ncbi.nlm.nih.gov/26379230
Autor:
Marcus Bantscheff, Fiona Pachl, Isabelle Becher, Toby Mathieson, Nico Zinn, Bernhard Kuster, Mikhail M. Savitski, Carola Doce, Gavain Sweetman
Publikováno v:
Journal of proteome research. 12(8)
Isobaric mass tagging (e.g., TMT and iTRAQ) is a precise and sensitive multiplexed peptide/protein quantification technique in mass spectrometry. However, accurate quantification of complex proteomic samples is impaired by cofragmentation of peptides
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90e2c32804334bd928daf473b9b4db07
https://pubmed.ncbi.nlm.nih.gov/23768245
https://pubmed.ncbi.nlm.nih.gov/23768245
Autor:
Katie Ellard, Marcus Bantscheff, Satoko Shimamura, Kathryn Bell, Carsten Hopf, Faiza Rharbaoui, Nigel Ramsden, Mikhail M. Savitski, Gerard Drewes, Gitte Neubauer, Raffaella Mangano, Andrew Cansfield, Toby Mathieson, Ivan Plavec, Christina Rau, Friedrich B M Reinhard, Daniel Leggate, Alison O'Mahony, Thilo Werner, Carola Doce, Jessica Perrin, Katrin Müller, Giovanna Bergamini, Oliver Rausch, Emilio Hirsch
Publikováno v:
Nature chemical biology. 8(6)
We devised a high-throughput chemoproteomics method that enabled multiplexed screening of 16,000 compounds against native protein and lipid kinases in cell extracts. Optimization of one chemical series resulted in CZC24832, which is to our knowledge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b747e5342fff30edde4865e7369029c5
https://pubmed.ncbi.nlm.nih.gov/22544264
https://pubmed.ncbi.nlm.nih.gov/22544264
Autor:
Carola Doce, Friedrich B M Reinhard, Thilo Werner, Raffaella Mangano, Satoko Shimamura, Katrin Müller, Nigel Ramsden, Gerard Drewes, Daniel Leggate, Gitte Neubauer, Jessica Perrin, Toby Mathieson, Alison O'Mahony, Marcus Bantscheff, Andrew Cansfield, Faiza Rharbaoui, Kathryn Bell, Carsten Hopf, Oliver Rausch, Mikhail M. Savitski, Ivan Plavec, Christina Rau, Katie Ellard, Giovanna Bergamini, Emilio Hirsch
Publikováno v:
Nature Chemical Biology. 8:737-737
Nat. Chem. Biol. 8, 576–582 (2012); published online 29 April 2012; corrected after print 16 May 2012 In the version of this article initially published, the name M. Sunose was misspelled in the Acknowledgements. The error has been corrected in the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::03c6db21d6695aadcb73d0425a7c3a4c
https://doi.org/10.1038/nchembio0812-737b
https://doi.org/10.1038/nchembio0812-737b