Nbs1 ChIP-Seq Identifies Off-Target DNA Double-Strand Breaks Induced by AID in Activated Splenic B Cells.

Autor: Lyne Khair, Richard E Baker, Erin K Linehan, Carol E Schrader, Janet Stavnezer

Publikováno v: PLoS Genetics, Vol 11, Iss 8, p e1005438 (2015)

Activation-induced cytidine deaminase (AID) is required for initiation of Ig class switch recombination (CSR) and somatic hypermutation (SHM) of antibody genes during immune responses. AID has also been shown to induce chromosomal translocations, mut

Externí odkaz: https://doaj.org/article/1ba46d83f23443f490a3690fd7e007c6

The DNA glycosylases Ogg1 and Nth1 do not contribute to Ig class switching in activated mouse splenic B cells.

Autor: Anna J Ucher, Erin K Linehan, George W Teebor, Carol E Schrader, Janet Stavnezer

Publikováno v: PLoS ONE, Vol 7, Iss 4, p e36061 (2012)

During activation of B cells to undergo class switching, B cell metabolism is increased, and levels of reactive oxygen species (ROS) are increased. ROS can oxidize DNA bases resulting in substrates for the DNA glycosylases Ogg1 and Nth1. Ogg1 and Nth

Externí odkaz: https://doaj.org/article/33c12d8efe1b4d5ebcaa67e2fcb34fc9

The DNA Damage Response Regulates RAG1/2 Expression in Pre–B Cells through ATM-FOXO1 Signaling

Autor: Jeroen E. J. Guikema, Timon A. Bloedjes, Carel J. M. van Noesel, Carol E. Schrader, Katarina Ochodnicka-Mackovicova, Richard J. Bende, Amélie van der Veen, Alexander M. de Bruin, Rudi W. Hendriks, Mahnoush Bahjat, Harmen van Andel, Chiel Maas, Els Verhoeyen

Publikováno v: Journal of Immunology (Baltimore, Md.: 1950)
Journal of Immunology (Baltimore, Md.: 1950), 2016, 197 (7), pp.2918-2929. ⟨10.4049/jimmunol.1501989⟩
Journal of Immunology, 197(7), 2918-2929. American Association of Immunologists
Journal of immunology (Baltimore, Md., 197(7), 2918-2929. American Association of Immunologists

The recombination activating gene (RAG) 1 and RAG2 protein complex introduces DNA breaks at Tcr and Ig gene segments that are required for V(D)J recombination in developing lymphocytes. Proper regulation of RAG1/2 expression safeguards the ordered as

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c8a0ba48b384592759e30a151850d88
https://doi.org/10.4049/jimmunol.1501989

ATM Increases Activation-Induced Cytidine Deaminase Activity at Downstream S Regions during Class-Switch Recombination

Autor: Janet Stavnezer, Erin K. Linehan, Jeroen E. J. Guikema, Zhenkun Lou, Lyne Khair, Colin Ogilvie, Carol E. Schrader, Niek G. J. Leus, Anna J. Ucher

Publikováno v: The Journal of Immunology, 192(10), 4887-4896. AMER ASSOC IMMUNOLOGISTS
Journal of immunology (Baltimore, Md., 192(10), 4887-4896. American Association of Immunologists

Activation-induced cytidine deaminase (AID) initiates Ab class-switch recombination (CSR) in activated B cells resulting in exchanging the IgH C region and improved Ab effector function. During CSR, AID instigates DNA double-strand break (DSB) format

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5777d810a5f02c81a2aea074d8fc47a
https://doi.org/10.4049/jimmunol.1303481

DNA polymerases β and λ do not directly affect Ig variable region somatic hypermutation although their absence reduces the frequency of mutations

Autor: Anna J. Ucher, Janet Stavnezer, Erin K. Linehan, Carol E. Schrader, Barbara Bertocci

Publikováno v: DNA Repair. 12:1087-1093

During somatic hypermutation (SHM) of antibody variable (V) region genes, activation-induced cytidine deaminase (AID) converts dC to dU, and dUs can either be excised by uracil DNA glycosylase (UNG), by mismatch repair, or replicated over. If UNG exc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0452f265e7cc41eb2d81e065ad1e6886
https://doi.org/10.1016/j.dnarep.2013.09.002